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Capillary electrophoresis hyphenated to inductively coupled plasma-sector field-mass spectrometry for the detection of chromium species after incubation of chromium in simulated sweat

Veerle Van Lierde, Cyrille C Chéry, Luc Moens UGent and Frank Vanhaecke UGent (2005) ELECTROPHORESIS. 26(9). p.1703-1711
abstract
The presence of chromium in chromium-tanned leather represents a considerable health problem since it can lead to chronic allergic contact dermatitis. Apart from trivalent chromium (Cr(III)), which is used for tanning, leather often contains hexavalent chromium (Cr(VI)), resulting from the oxidation of Cr(III) during the tanning process. This study deals With the chromium compounds in simulated sweat when brought into contact with Cr(III) or Cr(VI) and with chromium-tanned leathers. A capillary electrophoresis (CE) method was developed, with inductively coupled plasma-sector field-mass spectrometry (ICP-SF-MS) for element-specific detection. Two different electrophoretic runs, applying once the positive and once the negative polarity mode, were necessary for the detection of positively and negatively charged chromium species. Although sometimes described in the literature, a pre-run derivatization of the chromium-species was not performed here to prevent species transformation. 50 mmol(.)L(-1) sodium phosphate at a pH of 2.5 was used as CE separation buffer and as make-up liquid for the CE-ICP-SF-MS interface. When applied to simulated sweat samples incubated with Cr(VI), this method showed that methionine is responsible for the reduction of Cr(VI) into Cr(III), which, at its turn, forms a complex with lactic acid. In the case of sweat plus Cr(III), the latter step was also seen. Applied to simulated sweat in contact with leather samples, the method developed showed the presence of the former species among a much more complex pattern.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
chromium speciation, capillary electrophoresis, inductively coupled plasma-mass spectrometry, leather, simulated sweat, CONTACT-DERMATITIS, TANNED LEATHER, SPECIATION, ALLERGY, COMPLEXATION, OXIDATION, CHEMISTRY, EXPOSURE, CHROMATE, CR(VI)
journal title
ELECTROPHORESIS
Electrophoresis
volume
26
issue
9
pages
1703 - 1711
Web of Science type
Article
Web of Science id
000229135300011
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
3.85 (2005)
JCR rank
3/69 (2005)
JCR quartile
1 (2005)
ISSN
0173-0835
DOI
10.1002/elps.200410221
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
311228
handle
http://hdl.handle.net/1854/LU-311228
date created
2005-06-07 09:17:00
date last changed
2012-11-12 13:41:09
@article{311228,
  abstract     = {The presence of chromium in chromium-tanned leather represents a considerable health problem since it can lead to chronic allergic contact dermatitis. Apart from trivalent chromium (Cr(III)), which is used for tanning, leather often contains hexavalent chromium (Cr(VI)), resulting from the oxidation of Cr(III) during the tanning process. This study deals With the chromium compounds in simulated sweat when brought into contact with Cr(III) or Cr(VI) and with chromium-tanned leathers. A capillary electrophoresis (CE) method was developed, with inductively coupled plasma-sector field-mass spectrometry (ICP-SF-MS) for element-specific detection. Two different electrophoretic runs, applying once the positive and once the negative polarity mode, were necessary for the detection of positively and negatively charged chromium species. Although sometimes described in the literature, a pre-run derivatization of the chromium-species was not performed here to prevent species transformation. 50 mmol(.)L(-1) sodium phosphate at a pH of 2.5 was used as CE separation buffer and as make-up liquid for the CE-ICP-SF-MS interface. When applied to simulated sweat samples incubated with Cr(VI), this method showed that methionine is responsible for the reduction of Cr(VI) into Cr(III), which, at its turn, forms a complex with lactic acid. In the case of sweat plus Cr(III), the latter step was also seen. Applied to simulated sweat in contact with leather samples, the method developed showed the presence of the former species among a much more complex pattern.},
  author       = {Van Lierde, Veerle and Ch{\'e}ry, Cyrille C and Moens, Luc and Vanhaecke, Frank},
  issn         = {0173-0835},
  journal      = {ELECTROPHORESIS},
  keyword      = {chromium speciation,capillary electrophoresis,inductively coupled plasma-mass spectrometry,leather,simulated sweat,CONTACT-DERMATITIS,TANNED LEATHER,SPECIATION,ALLERGY,COMPLEXATION,OXIDATION,CHEMISTRY,EXPOSURE,CHROMATE,CR(VI)},
  language     = {eng},
  number       = {9},
  pages        = {1703--1711},
  title        = {Capillary electrophoresis hyphenated to inductively coupled plasma-sector field-mass spectrometry for the detection of chromium species after incubation of chromium in simulated sweat},
  url          = {http://dx.doi.org/10.1002/elps.200410221},
  volume       = {26},
  year         = {2005},
}

Chicago
Van Lierde, Veerle, Cyrille C Chéry, Luc Moens, and Frank Vanhaecke. 2005. “Capillary Electrophoresis Hyphenated to Inductively Coupled Plasma-sector Field-mass Spectrometry for the Detection of Chromium Species After Incubation of Chromium in Simulated Sweat.” Electrophoresis 26 (9): 1703–1711.
APA
Van Lierde, V., Chéry, C. C., Moens, L., & Vanhaecke, F. (2005). Capillary electrophoresis hyphenated to inductively coupled plasma-sector field-mass spectrometry for the detection of chromium species after incubation of chromium in simulated sweat. ELECTROPHORESIS, 26(9), 1703–1711.
Vancouver
1.
Van Lierde V, Chéry CC, Moens L, Vanhaecke F. Capillary electrophoresis hyphenated to inductively coupled plasma-sector field-mass spectrometry for the detection of chromium species after incubation of chromium in simulated sweat. ELECTROPHORESIS. 2005;26(9):1703–11.
MLA
Van Lierde, Veerle, Cyrille C Chéry, Luc Moens, et al. “Capillary Electrophoresis Hyphenated to Inductively Coupled Plasma-sector Field-mass Spectrometry for the Detection of Chromium Species After Incubation of Chromium in Simulated Sweat.” ELECTROPHORESIS 26.9 (2005): 1703–1711. Print.