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Renal function and morphology in aged Beagle dogs before and after hydrocortisone administration

Pascale Smets (UGent) , Hervé P Lefebvre, Luca Aresu, Siska Croubels (UGent) , Hendrik Haers (UGent) , Koen Piron (UGent) , Evelyne Meyer (UGent) and Sylvie Daminet (UGent)
(2012) PLOS ONE. 7(2).
Author
Organization
Abstract
Objectives of this study were to evaluate glomerular filtration rate (GFR), renal structural changes and proteinuria in aged Beagle dogs before and after hydrocortisone (HC) administration. Eleven Beagle dogs >= 10 years old were treated with either hydrocortisone (HC group, n = 6) or placebo (control group, n = 5). Urinary markers, GFR and kidney biopsies were evaluated before (T0), during (T16 wks) and after discontinuing HC administration (T24 wks). Results indicate that HC administration causes a significant increase in GFR. At all time points except T16 wks, proteinuria was higher in the control group than in the HC group, and there was no significant difference in urinary markers between groups. At T16 wks, proteinuria, urinary albumin-to-creatinine (c) ratio, immunoglobulin G/c and retinol-binding protein/c were higher compared to baseline in the HC group. At T0, rare to mild renal lesions were detected in all HC dogs and rare to moderate changes in all control dogs. Glomerulosclerosis progressed in both groups until T24 wks. Tubular atrophy was detected in three HC dogs at T16 wks and T24 wks, but also in five control dogs throughout the study. At every time point, five HC dogs and all control dogs had rare to moderate interstitial inflammation. Rare to mild interstitial fibrosis was found in up to three HC dogs at T16 wks and T24 wks, and severe fibrosis in one HC dog at T24 wks. Up to four control dogs had rare to mild fibrosis at all time points. These findings indicate that clinically healthy, aged Beagle dogs may have considerable renal lesions and proteinuria, which could have implications for experimental or toxicological studies. Additional research is needed to elucidate glucocorticoid effects on renal structure, but functional changes such as hyperfiltration and proteinuria warrant attention to kidney function of canine patients with Cushing's syndrome or receiving exogenous glucocorticoids.
Keywords
GLOMERULAR-FILTRATION-RATE, PITUITARY-DEPENDENT HYPERADRENOCORTICISM, RETINOL-BINDING-PROTEIN, CUSHINGS-DISEASE, URINE PROTEIN, EXOGENOUS CREATININE, GEL-ELECTROPHORESIS, ARTERIAL-PRESSURE, KIDNEY-DISEASE, BLOOD-PRESSURE

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MLA
Smets, Pascale, Hervé P Lefebvre, Luca Aresu, et al. “Renal Function and Morphology in Aged Beagle Dogs Before and After Hydrocortisone Administration.” PLOS ONE 7.2 (2012): n. pag. Print.
APA
Smets, P., Lefebvre, H. P., Aresu, L., Croubels, S., Haers, H., Piron, K., Meyer, E., et al. (2012). Renal function and morphology in aged Beagle dogs before and after hydrocortisone administration. PLOS ONE, 7(2).
Chicago author-date
Smets, Pascale, Hervé P Lefebvre, Luca Aresu, Siska Croubels, Hendrik Haers, Koen Piron, Evelyne Meyer, and Sylvie Daminet. 2012. “Renal Function and Morphology in Aged Beagle Dogs Before and After Hydrocortisone Administration.” Plos One 7 (2).
Chicago author-date (all authors)
Smets, Pascale, Hervé P Lefebvre, Luca Aresu, Siska Croubels, Hendrik Haers, Koen Piron, Evelyne Meyer, and Sylvie Daminet. 2012. “Renal Function and Morphology in Aged Beagle Dogs Before and After Hydrocortisone Administration.” Plos One 7 (2).
Vancouver
1.
Smets P, Lefebvre HP, Aresu L, Croubels S, Haers H, Piron K, et al. Renal function and morphology in aged Beagle dogs before and after hydrocortisone administration. PLOS ONE. 2012;7(2).
IEEE
[1]
P. Smets et al., “Renal function and morphology in aged Beagle dogs before and after hydrocortisone administration,” PLOS ONE, vol. 7, no. 2, 2012.
@article{3111645,
  abstract     = {Objectives of this study were to evaluate glomerular filtration rate (GFR), renal structural changes and proteinuria in aged Beagle dogs before and after hydrocortisone (HC) administration. Eleven Beagle dogs >= 10 years old were treated with either hydrocortisone (HC group, n = 6) or placebo (control group, n = 5). Urinary markers, GFR and kidney biopsies were evaluated before (T0), during (T16 wks) and after discontinuing HC administration (T24 wks). Results indicate that HC administration causes a significant increase in GFR. At all time points except T16 wks, proteinuria was higher in the control group than in the HC group, and there was no significant difference in urinary markers between groups. At T16 wks, proteinuria, urinary albumin-to-creatinine (c) ratio, immunoglobulin G/c and retinol-binding protein/c were higher compared to baseline in the HC group. At T0, rare to mild renal lesions were detected in all HC dogs and rare to moderate changes in all control dogs. Glomerulosclerosis progressed in both groups until T24 wks. Tubular atrophy was detected in three HC dogs at T16 wks and T24 wks, but also in five control dogs throughout the study. At every time point, five HC dogs and all control dogs had rare to moderate interstitial inflammation. Rare to mild interstitial fibrosis was found in up to three HC dogs at T16 wks and T24 wks, and severe fibrosis in one HC dog at T24 wks. Up to four control dogs had rare to mild fibrosis at all time points. These findings indicate that clinically healthy, aged Beagle dogs may have considerable renal lesions and proteinuria, which could have implications for experimental or toxicological studies. Additional research is needed to elucidate glucocorticoid effects on renal structure, but functional changes such as hyperfiltration and proteinuria warrant attention to kidney function of canine patients with Cushing's syndrome or receiving exogenous glucocorticoids.},
  articleno    = {e31702},
  author       = {Smets, Pascale and Lefebvre, Hervé P and Aresu, Luca and Croubels, Siska and Haers, Hendrik and Piron, Koen and Meyer, Evelyne and Daminet, Sylvie},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keywords     = {GLOMERULAR-FILTRATION-RATE,PITUITARY-DEPENDENT HYPERADRENOCORTICISM,RETINOL-BINDING-PROTEIN,CUSHINGS-DISEASE,URINE PROTEIN,EXOGENOUS CREATININE,GEL-ELECTROPHORESIS,ARTERIAL-PRESSURE,KIDNEY-DISEASE,BLOOD-PRESSURE},
  language     = {eng},
  number       = {2},
  pages        = {10},
  title        = {Renal function and morphology in aged Beagle dogs before and after hydrocortisone administration},
  url          = {http://dx.doi.org/10.1371/journal.pone.0031702},
  volume       = {7},
  year         = {2012},
}

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