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Abstract
In the present study, gelatin-based cryogels have been seeded with human SAOS-2 osteoblasts. In order to overcome the drawbacks associated with in vitro culture systems, such as limited diffusion and inhomogeneous cell-matrix distribution, this work describes the application of ultrasounds (average power, 149 mW; frequency, 1.5 MHz) to physically enhance the cell culture in vitro. The results indicate that the physical stimulation of cell-seeded gelatin-based cryogels upregulates the bone matrix production.
Keywords
POLYURETHANE POROUS SCAFFOLD, HUMAN SAOS-2 OSTEOBLASTS, CALCIFIED MATRIX DEPOSITION, PLASMA-SPRAY SURFACE, ELECTROMAGNETIC ENHANCEMENT, EXTRACELLULAR-MATRIX, HYDROGELS, CULTURE, CELLS, STIMULATION

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MLA
Fassina, Lorenzo, et al. “Ultrasound Stimulus to Enhance the Bone Regeneration Capability of Gelatin Cryogels.” IEEE Engineering in Medicine and Biology Society Conference Proceedings, IEEE, 2013, pp. 846–49, doi:10.1109/EMBC.2013.6609633.
APA
Fassina, L., Visai, L., Magenes, G., Schelfhout, J., Bloise, N., Riva, F., … Van Vlierberghe, S. (2013). Ultrasound stimulus to enhance the bone regeneration capability of gelatin cryogels. IEEE Engineering in Medicine and Biology Society Conference Proceedings, 846–849. https://doi.org/10.1109/EMBC.2013.6609633
Chicago author-date
Fassina, Lorenzo, Livia Visai, Giovanni Magenes, Jorg Schelfhout, Nora Bloise, Federica Riva, Claudia Omes, et al. 2013. “Ultrasound Stimulus to Enhance the Bone Regeneration Capability of Gelatin Cryogels.” In IEEE Engineering in Medicine and Biology Society Conference Proceedings, 846–49. New York, NY, USA: IEEE. https://doi.org/10.1109/EMBC.2013.6609633.
Chicago author-date (all authors)
Fassina, Lorenzo, Livia Visai, Giovanni Magenes, Jorg Schelfhout, Nora Bloise, Federica Riva, Claudia Omes, Maria Antonietta Avanzini, Maria Gabriella Cusella De Angelis, Francesco Benazzo, Manuel Dierick, Luc Van Hoorebeke, Peter Dubruel, and Sandra Van Vlierberghe. 2013. “Ultrasound Stimulus to Enhance the Bone Regeneration Capability of Gelatin Cryogels.” In IEEE Engineering in Medicine and Biology Society Conference Proceedings, 846–849. New York, NY, USA: IEEE. doi:10.1109/EMBC.2013.6609633.
Vancouver
1.
Fassina L, Visai L, Magenes G, Schelfhout J, Bloise N, Riva F, et al. Ultrasound stimulus to enhance the bone regeneration capability of gelatin cryogels. In: IEEE Engineering in Medicine and Biology Society Conference Proceedings. New York, NY, USA: IEEE; 2013. p. 846–9.
IEEE
[1]
L. Fassina et al., “Ultrasound stimulus to enhance the bone regeneration capability of gelatin cryogels,” in IEEE Engineering in Medicine and Biology Society Conference Proceedings, Osaka, Japan, 2013, pp. 846–849.
@inproceedings{3108264,
  abstract     = {{In the present study, gelatin-based cryogels have been seeded with human SAOS-2 osteoblasts. In order to overcome the drawbacks associated with in vitro culture systems, such as limited diffusion and inhomogeneous cell-matrix distribution, this work describes the application of ultrasounds (average power, 149 mW; frequency, 1.5 MHz) to physically enhance the cell culture in vitro. The results indicate that the physical stimulation of cell-seeded gelatin-based cryogels upregulates the bone matrix production.}},
  author       = {{Fassina, Lorenzo and Visai, Livia and Magenes, Giovanni and Schelfhout, Jorg and Bloise, Nora and Riva, Federica and Omes, Claudia and Avanzini, Maria Antonietta and De Angelis, Maria Gabriella Cusella and Benazzo, Francesco and Dierick, Manuel and Van Hoorebeke, Luc and Dubruel, Peter and Van Vlierberghe, Sandra}},
  booktitle    = {{IEEE Engineering in Medicine and Biology Society Conference Proceedings}},
  isbn         = {{9781457702167}},
  issn         = {{1557-170X}},
  keywords     = {{POLYURETHANE POROUS SCAFFOLD,HUMAN SAOS-2 OSTEOBLASTS,CALCIFIED MATRIX DEPOSITION,PLASMA-SPRAY SURFACE,ELECTROMAGNETIC ENHANCEMENT,EXTRACELLULAR-MATRIX,HYDROGELS,CULTURE,CELLS,STIMULATION}},
  language     = {{eng}},
  location     = {{Osaka, Japan}},
  pages        = {{846--849}},
  publisher    = {{IEEE}},
  title        = {{Ultrasound stimulus to enhance the bone regeneration capability of gelatin cryogels}},
  url          = {{http://doi.org/10.1109/EMBC.2013.6609633}},
  year         = {{2013}},
}

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