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A fluorescent alternative to the synthetic strigolactone GR24

(2013) MOLECULAR PLANT. 6(1). p.100-112
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Abstract
Strigolactones have recently been implicated in both above- and below-ground developmental pathways in higher plants. To facilitate the molecular and chemical properties of strigolactones in vitro and in vivo, we have developed a fluorescent strigolactone molecule, CISA-1, synthesized via a novel method which was robust, high-yielding, and used simple starting materials. We demonstrate that CISA-1 has a broad range of known strigolactone activities and further report on an adventitious rooting assay in Arabidopsis which is a highly sensitive and rapid method for testing biological activity of strigolactone analogs. In this rooting assay and the widely used Orobanche germination assay, CISA-1 showed stronger biological activity than the commonly tested GR24. CISA-1 and GR24 were equally effective at inhibiting branching in Arabidopsis inflorescence stems. In both the branching and adventitious rooting assay, we also demonstrated that CISA-1 activity is dependent on the max strigolactone signaling pathway. In water methanol solutions, CISA-1 was about threefold more stable than GR24, which may contribute to the increased activity observed in the various biological tests.
Keywords
STRUCTURAL MODIFICATIONS, ROOT DEVELOPMENT, SEED-GERMINATION, H-3 RECEPTOR LIGANDS, ARBUSCULAR MYCORRHIZAL FUNGI, parasitic weed seed germination, fluorescent markers, branching, strigolactones, adventitious rooting, ACTS DOWNSTREAM, ARABIDOPSIS, ANALOGS, INHIBITION, STRIGA

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Citation

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MLA
Rasmussen, Amanda, et al. “A Fluorescent Alternative to the Synthetic Strigolactone GR24.” MOLECULAR PLANT, vol. 6, no. 1, 2013, pp. 100–12.
APA
Rasmussen, A., Heugebaert, T., Matthys, C., Van Deun, R., Boyer, F.-D., Goormachtig, S., … Geelen, D. (2013). A fluorescent alternative to the synthetic strigolactone GR24. MOLECULAR PLANT, 6(1), 100–112.
Chicago author-date
Rasmussen, Amanda, Thomas Heugebaert, Cedrick Matthys, Rik Van Deun, Francois-Didier Boyer, Sofie Goormachtig, Christian Stevens, and Danny Geelen. 2013. “A Fluorescent Alternative to the Synthetic Strigolactone GR24.” MOLECULAR PLANT 6 (1): 100–112.
Chicago author-date (all authors)
Rasmussen, Amanda, Thomas Heugebaert, Cedrick Matthys, Rik Van Deun, Francois-Didier Boyer, Sofie Goormachtig, Christian Stevens, and Danny Geelen. 2013. “A Fluorescent Alternative to the Synthetic Strigolactone GR24.” MOLECULAR PLANT 6 (1): 100–112.
Vancouver
1.
Rasmussen A, Heugebaert T, Matthys C, Van Deun R, Boyer F-D, Goormachtig S, et al. A fluorescent alternative to the synthetic strigolactone GR24. MOLECULAR PLANT. 2013;6(1):100–12.
IEEE
[1]
A. Rasmussen et al., “A fluorescent alternative to the synthetic strigolactone GR24,” MOLECULAR PLANT, vol. 6, no. 1, pp. 100–112, 2013.
@article{3103390,
  abstract     = {Strigolactones have recently been implicated in both above- and below-ground developmental pathways in higher plants. To facilitate the molecular and chemical properties of strigolactones in vitro and in vivo, we have developed a fluorescent strigolactone molecule, CISA-1, synthesized via a novel method which was robust, high-yielding, and used simple starting materials. We demonstrate that CISA-1 has a broad range of known strigolactone activities and further report on an adventitious rooting assay in Arabidopsis which is a highly sensitive and rapid method for testing biological activity of strigolactone analogs. In this rooting assay and the widely used Orobanche germination assay, CISA-1 showed stronger biological activity than the commonly tested GR24. CISA-1 and GR24 were equally effective at inhibiting branching in Arabidopsis inflorescence stems. In both the branching and adventitious rooting assay, we also demonstrated that CISA-1 activity is dependent on the max strigolactone signaling pathway. In water methanol solutions, CISA-1 was about threefold more stable than GR24, which may contribute to the increased activity observed in the various biological tests.},
  author       = {Rasmussen, Amanda and Heugebaert, Thomas and Matthys, Cedrick and Van Deun, Rik and Boyer, Francois-Didier and Goormachtig, Sofie and Stevens, Christian and Geelen, Danny},
  issn         = {1674-2052},
  journal      = {MOLECULAR PLANT},
  keywords     = {STRUCTURAL MODIFICATIONS,ROOT DEVELOPMENT,SEED-GERMINATION,H-3 RECEPTOR LIGANDS,ARBUSCULAR MYCORRHIZAL FUNGI,parasitic weed seed germination,fluorescent markers,branching,strigolactones,adventitious rooting,ACTS DOWNSTREAM,ARABIDOPSIS,ANALOGS,INHIBITION,STRIGA},
  language     = {eng},
  number       = {1},
  pages        = {100--112},
  title        = {A fluorescent alternative to the synthetic strigolactone GR24},
  url          = {http://dx.doi.org/10.1093/mp/sss110},
  volume       = {6},
  year         = {2013},
}

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