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A fluorescent alternative to the synthetic strigolactone GR24

(2013) MOLECULAR PLANT. 6(1). p.100-112
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Abstract
Strigolactones have recently been implicated in both above- and below-ground developmental pathways in higher plants. To facilitate the molecular and chemical properties of strigolactones in vitro and in vivo, we have developed a fluorescent strigolactone molecule, CISA-1, synthesized via a novel method which was robust, high-yielding, and used simple starting materials. We demonstrate that CISA-1 has a broad range of known strigolactone activities and further report on an adventitious rooting assay in Arabidopsis which is a highly sensitive and rapid method for testing biological activity of strigolactone analogs. In this rooting assay and the widely used Orobanche germination assay, CISA-1 showed stronger biological activity than the commonly tested GR24. CISA-1 and GR24 were equally effective at inhibiting branching in Arabidopsis inflorescence stems. In both the branching and adventitious rooting assay, we also demonstrated that CISA-1 activity is dependent on the max strigolactone signaling pathway. In water methanol solutions, CISA-1 was about threefold more stable than GR24, which may contribute to the increased activity observed in the various biological tests.
Keywords
STRUCTURAL MODIFICATIONS, ROOT DEVELOPMENT, SEED-GERMINATION, H-3 RECEPTOR LIGANDS, ARBUSCULAR MYCORRHIZAL FUNGI, parasitic weed seed germination, fluorescent markers, branching, strigolactones, adventitious rooting, ACTS DOWNSTREAM, ARABIDOPSIS, ANALOGS, INHIBITION, STRIGA

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Chicago
Rasmussen, Amanda, Thomas Heugebaert, Cedrick Matthys, Rik Van Deun, Francois-Didier Boyer, Sofie Goormachtig, Christian Stevens, and Danny Geelen. 2013. “A Fluorescent Alternative to the Synthetic Strigolactone GR24.” Molecular Plant 6 (1): 100–112.
APA
Rasmussen, A., Heugebaert, T., Matthys, C., Van Deun, R., Boyer, F.-D., Goormachtig, S., Stevens, C., et al. (2013). A fluorescent alternative to the synthetic strigolactone GR24. MOLECULAR PLANT, 6(1), 100–112.
Vancouver
1.
Rasmussen A, Heugebaert T, Matthys C, Van Deun R, Boyer F-D, Goormachtig S, et al. A fluorescent alternative to the synthetic strigolactone GR24. MOLECULAR PLANT. 2013;6(1):100–12.
MLA
Rasmussen, Amanda, Thomas Heugebaert, Cedrick Matthys, et al. “A Fluorescent Alternative to the Synthetic Strigolactone GR24.” MOLECULAR PLANT 6.1 (2013): 100–112. Print.
@article{3103390,
  abstract     = {Strigolactones have recently been implicated in both above- and below-ground developmental pathways in higher plants. To facilitate the molecular and chemical properties of strigolactones in vitro and in vivo, we have developed a fluorescent strigolactone molecule, CISA-1, synthesized via a novel method which was robust, high-yielding, and used simple starting materials. We demonstrate that CISA-1 has a broad range of known strigolactone activities and further report on an adventitious rooting assay in Arabidopsis which is a highly sensitive and rapid method for testing biological activity of strigolactone analogs. In this rooting assay and the widely used Orobanche germination assay, CISA-1 showed stronger biological activity than the commonly tested GR24. CISA-1 and GR24 were equally effective at inhibiting branching in Arabidopsis inflorescence stems. In both the branching and adventitious rooting assay, we also demonstrated that CISA-1 activity is dependent on the max strigolactone signaling pathway. In water methanol solutions, CISA-1 was about threefold more stable than GR24, which may contribute to the increased activity observed in the various biological tests.},
  author       = {Rasmussen, Amanda and Heugebaert, Thomas and Matthys, Cedrick and Van Deun, Rik and Boyer, Francois-Didier and Goormachtig, Sofie and Stevens, Christian and Geelen, Danny},
  issn         = {1674-2052},
  journal      = {MOLECULAR PLANT},
  keyword      = {STRUCTURAL MODIFICATIONS,ROOT DEVELOPMENT,SEED-GERMINATION,H-3 RECEPTOR LIGANDS,ARBUSCULAR MYCORRHIZAL FUNGI,parasitic weed seed germination,fluorescent markers,branching,strigolactones,adventitious rooting,ACTS DOWNSTREAM,ARABIDOPSIS,ANALOGS,INHIBITION,STRIGA},
  language     = {eng},
  number       = {1},
  pages        = {100--112},
  title        = {A fluorescent alternative to the synthetic strigolactone GR24},
  url          = {http://dx.doi.org/10.1093/mp/sss110},
  volume       = {6},
  year         = {2013},
}

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