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Bacillus berkeleyi sp. nov., isolated from the sea urchin Strongylocentrotus intermedius

Olga I Nedashkovskaya, Stefanie Van Trappen (UGent) , Galina M Frolova and Paul De Vos (UGent)
(2012) ARCHIVES OF MICROBIOLOGY. 194(3). p.215-221
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Abstract
A bacterial strain, designated KMM 6244(T), was isolated from the sea urchin Strongylocentrotus intermedius and subjected to a polyphasic taxonomic investigation. The bacterium was found to be heterotrophic, aerobic, non-motile and spore-forming. Comparative phylogenetic analysis based on 16S rRNA gene sequencing placed the marine isolate in the genus Bacillus. The nearest neighbor of strain KMM 6244(T) was Bacillus decolorationis LMG 19507(T) with a 16S rRNA gene sequence similarity of 98.0%. Sequence similarities with the other recognized Bacillus species were less than 96.0%. The results of the DNA-DNA hybridization experiments revealed a low relatedness (37%) of the novel isolate with the type strain of B. decolorationis LMG 19507(T). Strain KMM 6244(T) grew at 4-45A degrees C and with 0-12% NaCl. It produced catalase and oxidase and hydrolyzed aesculin, casein, gelatin and DNA. The predominant fatty acids were anteiso-C-15:0, iso-C-15:0, anteiso-C-17:0, C-15:0, iso-C-16:0 and iso-C-14:0. The DNA G + C content was 39.4 mol%. A combination of phylogenetic, genotypic and phenotypic data clearly indicated that strain KMM 6244(T) represents a novel species in the genus Bacillus, for which the name Bacillus berkeleyi sp. nov. is proposed. The type strain is KMM 6244(T) (KCTC 12718(T) = LMG 26357(T)).
Keywords
Aerobic marine spore-forming bacteria, Bacillus berkeleyi, Phylogeny, Taxonomy, RIBOSOMAL-RNA GROUP-6, DEOXYRIBONUCLEIC ACID, YELLOW SEA, BACTERIUM, KOREA, SEDIMENTS, STRAINS, MEMBERS

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Citation

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MLA
Nedashkovskaya, Olga I., et al. “Bacillus Berkeleyi Sp. Nov., Isolated from the Sea Urchin Strongylocentrotus Intermedius.” ARCHIVES OF MICROBIOLOGY, vol. 194, no. 3, 2012, pp. 215–21, doi:10.1007/s00203-011-0771-0.
APA
Nedashkovskaya, O. I., Van Trappen, S., Frolova, G. M., & De Vos, P. (2012). Bacillus berkeleyi sp. nov., isolated from the sea urchin Strongylocentrotus intermedius. ARCHIVES OF MICROBIOLOGY, 194(3), 215–221. https://doi.org/10.1007/s00203-011-0771-0
Chicago author-date
Nedashkovskaya, Olga I, Stefanie Van Trappen, Galina M Frolova, and Paul De Vos. 2012. “Bacillus Berkeleyi Sp. Nov., Isolated from the Sea Urchin Strongylocentrotus Intermedius.” ARCHIVES OF MICROBIOLOGY 194 (3): 215–21. https://doi.org/10.1007/s00203-011-0771-0.
Chicago author-date (all authors)
Nedashkovskaya, Olga I, Stefanie Van Trappen, Galina M Frolova, and Paul De Vos. 2012. “Bacillus Berkeleyi Sp. Nov., Isolated from the Sea Urchin Strongylocentrotus Intermedius.” ARCHIVES OF MICROBIOLOGY 194 (3): 215–221. doi:10.1007/s00203-011-0771-0.
Vancouver
1.
Nedashkovskaya OI, Van Trappen S, Frolova GM, De Vos P. Bacillus berkeleyi sp. nov., isolated from the sea urchin Strongylocentrotus intermedius. ARCHIVES OF MICROBIOLOGY. 2012;194(3):215–21.
IEEE
[1]
O. I. Nedashkovskaya, S. Van Trappen, G. M. Frolova, and P. De Vos, “Bacillus berkeleyi sp. nov., isolated from the sea urchin Strongylocentrotus intermedius,” ARCHIVES OF MICROBIOLOGY, vol. 194, no. 3, pp. 215–221, 2012.
@article{3102784,
  abstract     = {{A bacterial strain, designated KMM 6244(T), was isolated from the sea urchin Strongylocentrotus intermedius and subjected to a polyphasic taxonomic investigation. The bacterium was found to be heterotrophic, aerobic, non-motile and spore-forming. Comparative phylogenetic analysis based on 16S rRNA gene sequencing placed the marine isolate in the genus Bacillus. The nearest neighbor of strain KMM 6244(T) was Bacillus decolorationis LMG 19507(T) with a 16S rRNA gene sequence similarity of 98.0%. Sequence similarities with the other recognized Bacillus species were less than 96.0%. The results of the DNA-DNA hybridization experiments revealed a low relatedness (37%) of the novel isolate with the type strain of B. decolorationis LMG 19507(T). Strain KMM 6244(T) grew at 4-45A degrees C and with 0-12% NaCl. It produced catalase and oxidase and hydrolyzed aesculin, casein, gelatin and DNA. The predominant fatty acids were anteiso-C-15:0, iso-C-15:0, anteiso-C-17:0, C-15:0, iso-C-16:0 and iso-C-14:0. The DNA G + C content was 39.4 mol%. A combination of phylogenetic, genotypic and phenotypic data clearly indicated that strain KMM 6244(T) represents a novel species in the genus Bacillus, for which the name Bacillus berkeleyi sp. nov. is proposed. The type strain is KMM 6244(T) (KCTC 12718(T) = LMG 26357(T)).}},
  author       = {{Nedashkovskaya, Olga I and Van Trappen, Stefanie and Frolova, Galina M and De Vos, Paul}},
  issn         = {{0302-8933}},
  journal      = {{ARCHIVES OF MICROBIOLOGY}},
  keywords     = {{Aerobic marine spore-forming bacteria,Bacillus berkeleyi,Phylogeny,Taxonomy,RIBOSOMAL-RNA GROUP-6,DEOXYRIBONUCLEIC ACID,YELLOW SEA,BACTERIUM,KOREA,SEDIMENTS,STRAINS,MEMBERS}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{215--221}},
  title        = {{Bacillus berkeleyi sp. nov., isolated from the sea urchin Strongylocentrotus intermedius}},
  url          = {{http://doi.org/10.1007/s00203-011-0771-0}},
  volume       = {{194}},
  year         = {{2012}},
}
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