Advanced search
1 file | 268.56 KB

Bacillus berkeleyi sp. nov., isolated from the sea urchin Strongylocentrotus intermedius

(2012) ARCHIVES OF MICROBIOLOGY. 194(3). p.215-221
Author
Organization
Abstract
A bacterial strain, designated KMM 6244(T), was isolated from the sea urchin Strongylocentrotus intermedius and subjected to a polyphasic taxonomic investigation. The bacterium was found to be heterotrophic, aerobic, non-motile and spore-forming. Comparative phylogenetic analysis based on 16S rRNA gene sequencing placed the marine isolate in the genus Bacillus. The nearest neighbor of strain KMM 6244(T) was Bacillus decolorationis LMG 19507(T) with a 16S rRNA gene sequence similarity of 98.0%. Sequence similarities with the other recognized Bacillus species were less than 96.0%. The results of the DNA-DNA hybridization experiments revealed a low relatedness (37%) of the novel isolate with the type strain of B. decolorationis LMG 19507(T). Strain KMM 6244(T) grew at 4-45A degrees C and with 0-12% NaCl. It produced catalase and oxidase and hydrolyzed aesculin, casein, gelatin and DNA. The predominant fatty acids were anteiso-C-15:0, iso-C-15:0, anteiso-C-17:0, C-15:0, iso-C-16:0 and iso-C-14:0. The DNA G + C content was 39.4 mol%. A combination of phylogenetic, genotypic and phenotypic data clearly indicated that strain KMM 6244(T) represents a novel species in the genus Bacillus, for which the name Bacillus berkeleyi sp. nov. is proposed. The type strain is KMM 6244(T) (KCTC 12718(T) = LMG 26357(T)).
Keywords
Aerobic marine spore-forming bacteria, Bacillus berkeleyi, Phylogeny, Taxonomy, RIBOSOMAL-RNA GROUP-6, DEOXYRIBONUCLEIC ACID, YELLOW SEA, BACTERIUM, KOREA, SEDIMENTS, STRAINS, MEMBERS

Downloads

    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 268.56 KB

Citation

Please use this url to cite or link to this publication:

Chicago
Nedashkovskaya, Olga I, Stefanie Van Trappen, Galina M Frolova, and Paul De Vos. 2012. “Bacillus Berkeleyi Sp. Nov., Isolated from the Sea Urchin Strongylocentrotus Intermedius.” Archives of Microbiology 194 (3): 215–221.
APA
Nedashkovskaya, O. I., Van Trappen, S., Frolova, G. M., & De Vos, P. (2012). Bacillus berkeleyi sp. nov., isolated from the sea urchin Strongylocentrotus intermedius. ARCHIVES OF MICROBIOLOGY, 194(3), 215–221.
Vancouver
1.
Nedashkovskaya OI, Van Trappen S, Frolova GM, De Vos P. Bacillus berkeleyi sp. nov., isolated from the sea urchin Strongylocentrotus intermedius. ARCHIVES OF MICROBIOLOGY. 2012;194(3):215–21.
MLA
Nedashkovskaya, Olga I, Stefanie Van Trappen, Galina M Frolova, et al. “Bacillus Berkeleyi Sp. Nov., Isolated from the Sea Urchin Strongylocentrotus Intermedius.” ARCHIVES OF MICROBIOLOGY 194.3 (2012): 215–221. Print.
@article{3102784,
  abstract     = {A bacterial strain, designated KMM 6244(T), was isolated from the sea urchin Strongylocentrotus intermedius and subjected to a polyphasic taxonomic investigation. The bacterium was found to be heterotrophic, aerobic, non-motile and spore-forming. Comparative phylogenetic analysis based on 16S rRNA gene sequencing placed the marine isolate in the genus Bacillus. The nearest neighbor of strain KMM 6244(T) was Bacillus decolorationis LMG 19507(T) with a 16S rRNA gene sequence similarity of 98.0\%. Sequence similarities with the other recognized Bacillus species were less than 96.0\%. The results of the DNA-DNA hybridization experiments revealed a low relatedness (37\%) of the novel isolate with the type strain of B. decolorationis LMG 19507(T). Strain KMM 6244(T) grew at 4-45A degrees C and with 0-12\% NaCl. It produced catalase and oxidase and hydrolyzed aesculin, casein, gelatin and DNA. The predominant fatty acids were anteiso-C-15:0, iso-C-15:0, anteiso-C-17:0, C-15:0, iso-C-16:0 and iso-C-14:0. The DNA G + C content was 39.4 mol\%. A combination of phylogenetic, genotypic and phenotypic data clearly indicated that strain KMM 6244(T) represents a novel species in the genus Bacillus, for which the name Bacillus berkeleyi sp. nov. is proposed. The type strain is KMM 6244(T) (KCTC 12718(T) = LMG 26357(T)).},
  author       = {Nedashkovskaya, Olga I and Van Trappen, Stefanie and Frolova, Galina M and De Vos, Paul},
  issn         = {0302-8933},
  journal      = {ARCHIVES OF MICROBIOLOGY},
  keyword      = {Aerobic marine spore-forming bacteria,Bacillus berkeleyi,Phylogeny,Taxonomy,RIBOSOMAL-RNA GROUP-6,DEOXYRIBONUCLEIC ACID,YELLOW SEA,BACTERIUM,KOREA,SEDIMENTS,STRAINS,MEMBERS},
  language     = {eng},
  number       = {3},
  pages        = {215--221},
  title        = {Bacillus berkeleyi sp. nov., isolated from the sea urchin Strongylocentrotus intermedius},
  url          = {http://dx.doi.org/10.1007/s00203-011-0771-0},
  volume       = {194},
  year         = {2012},
}

Altmetric
View in Altmetric
Web of Science
Times cited: