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Enterococcus rivorum sp. nov., from water of pristine brooks

R Maarit Niemi, Tuula Ollinkangas, Lars Paulin, Pavel Švec, Peter Vandamme UGent, Antti Karkman, Marcel Kosina and Kristina Lindström (2012) INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 62(9). p.2169-2173
abstract
A significant number of Enterococcus strains from pristine waters of two brooks in Finland formed a distinct cluster on the basis of whole-cell protein fingerprinting by one-dimensional SDS-PAGE. The strains shared the following characteristics. Cells were ovoid, Gram-positive-staining and non-spore-forming, appearing singly or in pairs or chains. They were facultatively anaerobic and catalase-negative. Growth in broth containing 6.5% NaCl or at 45 degrees C was weak or absent. Production of D antigen was variable. The strains tolerated 60 degrees C for 30 min, 40% bile and tellurite, hydrolysed aesculin strongly and gelatin weakly, produced no acid from hippurate and did not reduce it, grew weakly at 10 degrees C, showed a strong reaction for the Voges-Proskauer test and produced acid from methyl alpha-D-glucoside, mannitol, sorbitol and sucrose, with weak or no production of acid from methyl alpha-D-mannoside, L-arabinose, gluconate and L-xylose. Several of the strains were selected for identification on the basis of sequencing of almost the whole 16S rRNA gene and partial atpA and pheS genes and of (GTG)(5)-PCR fingerprints. Partial atpA and pheS gene sequencing was also performed for those type strains of Enterococcus species without available sequences in the database. The pristine brook isolates formed a novel species, for which the name Enterococcus rivorum sp. nov. (type strain S299(T) =HAMBI 3055(T) =LMG 25899(T) =CCM 7986(T)) is proposed. On the basis of 16S rRNA gene sequence similarity, E. rivorum sp. nov. is related to the Enterococcus faecalis genogoup. It is distinguished from described Enterococcus species on the basis of 16S rRNA, atpA and pheS gene sequences and whole-cell protein and (GTG)(5)-PCR fingerprints. It is most closely related to E. faecalis, but DNA-DNA hybridization confirms it to represent a novel species.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
RNA, DNA, SEQUENCE-ANALYSIS, RAPID IDENTIFICATION, GENES, HYBRIDIZATION
journal title
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
Int. J. Syst. Evol. Microbiol.
volume
62
issue
9
pages
2169 - 2173
Web of Science type
Article
Web of Science id
000310048600020
JCR category
MICROBIOLOGY
JCR impact factor
2.112 (2012)
JCR rank
68/116 (2012)
JCR quartile
3 (2012)
ISSN
1466-5026
DOI
10.1099/ijs.0.038257-0
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
3102040
handle
http://hdl.handle.net/1854/LU-3102040
date created
2013-01-21 11:30:55
date last changed
2016-12-19 15:45:34
@article{3102040,
  abstract     = {A significant number of Enterococcus strains from pristine waters of two brooks in Finland formed a distinct cluster on the basis of whole-cell protein fingerprinting by one-dimensional SDS-PAGE. The strains shared the following characteristics. Cells were ovoid, Gram-positive-staining and non-spore-forming, appearing singly or in pairs or chains. They were facultatively anaerobic and catalase-negative. Growth in broth containing 6.5\% NaCl or at 45 degrees C was weak or absent. Production of D antigen was variable. The strains tolerated 60 degrees C for 30 min, 40\% bile and tellurite, hydrolysed aesculin strongly and gelatin weakly, produced no acid from hippurate and did not reduce it, grew weakly at 10 degrees C, showed a strong reaction for the Voges-Proskauer test and produced acid from methyl alpha-D-glucoside, mannitol, sorbitol and sucrose, with weak or no production of acid from methyl alpha-D-mannoside, L-arabinose, gluconate and L-xylose. Several of the strains were selected for identification on the basis of sequencing of almost the whole 16S rRNA gene and partial atpA and pheS genes and of (GTG)(5)-PCR fingerprints. Partial atpA and pheS gene sequencing was also performed for those type strains of Enterococcus species without available sequences in the database. The pristine brook isolates formed a novel species, for which the name Enterococcus rivorum sp. nov. (type strain S299(T) =HAMBI 3055(T) =LMG 25899(T) =CCM 7986(T)) is proposed. On the basis of 16S rRNA gene sequence similarity, E. rivorum sp. nov. is related to the Enterococcus faecalis genogoup. It is distinguished from described Enterococcus species on the basis of 16S rRNA, atpA and pheS gene sequences and whole-cell protein and (GTG)(5)-PCR fingerprints. It is most closely related to E. faecalis, but DNA-DNA hybridization confirms it to represent a novel species.},
  author       = {Niemi, R Maarit and Ollinkangas, Tuula and Paulin, Lars and \v{S}vec, Pavel and Vandamme, Peter and Karkman, Antti and Kosina, Marcel and Lindstr{\"o}m, Kristina},
  issn         = {1466-5026},
  journal      = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
  keyword      = {RNA,DNA,SEQUENCE-ANALYSIS,RAPID IDENTIFICATION,GENES,HYBRIDIZATION},
  language     = {eng},
  number       = {9},
  pages        = {2169--2173},
  title        = {Enterococcus rivorum sp. nov., from water of pristine brooks},
  url          = {http://dx.doi.org/10.1099/ijs.0.038257-0},
  volume       = {62},
  year         = {2012},
}

Chicago
Niemi, R Maarit, Tuula Ollinkangas, Lars Paulin, Pavel Švec, Peter Vandamme, Antti Karkman, Marcel Kosina, and Kristina Lindström. 2012. “Enterococcus Rivorum Sp. Nov., from Water of Pristine Brooks.” International Journal of Systematic and Evolutionary Microbiology 62 (9): 2169–2173.
APA
Niemi, R. M., Ollinkangas, T., Paulin, L., Švec, P., Vandamme, P., Karkman, A., Kosina, M., et al. (2012). Enterococcus rivorum sp. nov., from water of pristine brooks. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 62(9), 2169–2173.
Vancouver
1.
Niemi RM, Ollinkangas T, Paulin L, Švec P, Vandamme P, Karkman A, et al. Enterococcus rivorum sp. nov., from water of pristine brooks. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2012;62(9):2169–73.
MLA
Niemi, R Maarit, Tuula Ollinkangas, Lars Paulin, et al. “Enterococcus Rivorum Sp. Nov., from Water of Pristine Brooks.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 62.9 (2012): 2169–2173. Print.