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Characterization of coagulase-negative staphylococcus species from cows' milk and environment based on bap, icaA, and mecA genes and phenotypic susceptibility to antimicrobials and teat dips

(2012) JOURNAL OF DAIRY SCIENCE. 95(12). p.7027-7038
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Abstract
The aim of this study was to investigate whether the main coagulase-negative staphylococcus (CNS) species involved in bovine intramammary infections (Evil) possess specific characteristics that promote colonization of the udder. Virulence markers associated with biofilm formation, antimicrobial resistance, and biocide tolerance were compared between typically contagious CNS species (Staphylococcus chromogenes, Staphylococcus epidermidis, Staphylococcus haemolyticus, and Staphylococcus simulans) and those rarely causing IMI (Staphylococcus sciuri, Staphylococcus equorum, and others) to find possible associations with pathogenicity. Coagulase-negative staphylococcus isolates (n = 366) belonging to 22 different species were analyzed by PCR for the presence of the biofilm-associated genes bap and icaA, and the methicillin resistance gene mecA. A selection of 82 isolates was additionally tested for their susceptibility to 5 antibiotics and 2 commercial teat clip products. Minimum inhibitory concentrations of antimicrobials were determined by Etest (AB bio-Merieux, Marcy l'Etoile, France), and a microdilution method was optimized to determine minimum biocidal concentrations of teat dips. The bap, icaA, and mecA genes were detected significantly more in isolates from CNS species typically living in the cows' environment than in isolates from Evil-causing species. Antimicrobial resistance was mainly against erythromycin (23%) or oxacillin (16%), and was detected more often in the environmental species. The isolates least susceptible to the teat clips belonged to the IMI-causing species Staph. chromogenes and Staph. simulans. We concluded that carriage of biofilm genes and antimicrobial resistance were not associated with the ability to colonize the mammary gland because free-living CNS species constituted a more significant reservoir of biofilm and resistance determinants than did IMI-causing species. In contrast, increased tolerance to biocides may favor the establishment of bovine IMI by some CNS species.
Keywords
INTRAMAMMARY INFECTION, METHICILLIN RESISTANCE, DAIRY-COWS, BIOFILM FORMATION, BOVINE SUBCLINICAL MASTITIS, intramammary infection, coagulase-negative staphylococcus, antimicrobial, biofilm, AUREUS, PREVALENCE, IDENTIFICATION, PATHOGENS, BACTERIA

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Citation

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MLA
Piessens, Veerle, Sarne De Vliegher, Bert Verbist, et al. “Characterization of Coagulase-negative Staphylococcus Species from Cows’ Milk and Environment Based on Bap, icaA, and mecA Genes and Phenotypic Susceptibility to Antimicrobials and Teat Dips.” JOURNAL OF DAIRY SCIENCE 95.12 (2012): 7027–7038. Print.
APA
Piessens, V., De Vliegher, S., Verbist, B., Braem, G., Van Nuffel, A., De Vuyst, L., Heyndrickx, M., et al. (2012). Characterization of coagulase-negative staphylococcus species from cows’ milk and environment based on bap, icaA, and mecA genes and phenotypic susceptibility to antimicrobials and teat dips. JOURNAL OF DAIRY SCIENCE, 95(12), 7027–7038.
Chicago author-date
Piessens, Veerle, Sarne De Vliegher, Bert Verbist, G Braem, A Van Nuffel, L De Vuyst, M Heyndrickx, and Els Van Coillie. 2012. “Characterization of Coagulase-negative Staphylococcus Species from Cows’ Milk and Environment Based on Bap, icaA, and mecA Genes and Phenotypic Susceptibility to Antimicrobials and Teat Dips.” Journal of Dairy Science 95 (12): 7027–7038.
Chicago author-date (all authors)
Piessens, Veerle, Sarne De Vliegher, Bert Verbist, G Braem, A Van Nuffel, L De Vuyst, M Heyndrickx, and Els Van Coillie. 2012. “Characterization of Coagulase-negative Staphylococcus Species from Cows’ Milk and Environment Based on Bap, icaA, and mecA Genes and Phenotypic Susceptibility to Antimicrobials and Teat Dips.” Journal of Dairy Science 95 (12): 7027–7038.
Vancouver
1.
Piessens V, De Vliegher S, Verbist B, Braem G, Van Nuffel A, De Vuyst L, et al. Characterization of coagulase-negative staphylococcus species from cows’ milk and environment based on bap, icaA, and mecA genes and phenotypic susceptibility to antimicrobials and teat dips. JOURNAL OF DAIRY SCIENCE. 2012;95(12):7027–38.
IEEE
[1]
V. Piessens et al., “Characterization of coagulase-negative staphylococcus species from cows’ milk and environment based on bap, icaA, and mecA genes and phenotypic susceptibility to antimicrobials and teat dips,” JOURNAL OF DAIRY SCIENCE, vol. 95, no. 12, pp. 7027–7038, 2012.
@article{3098688,
  abstract     = {The aim of this study was to investigate whether the main coagulase-negative staphylococcus (CNS) species involved in bovine intramammary infections (Evil) possess specific characteristics that promote colonization of the udder. Virulence markers associated with biofilm formation, antimicrobial resistance, and biocide tolerance were compared between typically contagious CNS species (Staphylococcus chromogenes, Staphylococcus epidermidis, Staphylococcus haemolyticus, and Staphylococcus simulans) and those rarely causing IMI (Staphylococcus sciuri, Staphylococcus equorum, and others) to find possible associations with pathogenicity. Coagulase-negative staphylococcus isolates (n = 366) belonging to 22 different species were analyzed by PCR for the presence of the biofilm-associated genes bap and icaA, and the methicillin resistance gene mecA. A selection of 82 isolates was additionally tested for their susceptibility to 5 antibiotics and 2 commercial teat clip products. Minimum inhibitory concentrations of antimicrobials were determined by Etest (AB bio-Merieux, Marcy l'Etoile, France), and a microdilution method was optimized to determine minimum biocidal concentrations of teat dips. The bap, icaA, and mecA genes were detected significantly more in isolates from CNS species typically living in the cows' environment than in isolates from Evil-causing species. Antimicrobial resistance was mainly against erythromycin (23%) or oxacillin (16%), and was detected more often in the environmental species. The isolates least susceptible to the teat clips belonged to the IMI-causing species Staph. chromogenes and Staph. simulans. We concluded that carriage of biofilm genes and antimicrobial resistance were not associated with the ability to colonize the mammary gland because free-living CNS species constituted a more significant reservoir of biofilm and resistance determinants than did IMI-causing species. In contrast, increased tolerance to biocides may favor the establishment of bovine IMI by some CNS species.},
  author       = {Piessens, Veerle and De Vliegher, Sarne and Verbist, Bert and Braem, G and Van Nuffel, A and De Vuyst, L and Heyndrickx, M and Van Coillie, Els},
  issn         = {0022-0302},
  journal      = {JOURNAL OF DAIRY SCIENCE},
  keywords     = {INTRAMAMMARY INFECTION,METHICILLIN RESISTANCE,DAIRY-COWS,BIOFILM FORMATION,BOVINE SUBCLINICAL MASTITIS,intramammary infection,coagulase-negative staphylococcus,antimicrobial,biofilm,AUREUS,PREVALENCE,IDENTIFICATION,PATHOGENS,BACTERIA},
  language     = {eng},
  number       = {12},
  pages        = {7027--7038},
  title        = {Characterization of coagulase-negative staphylococcus species from cows' milk and environment based on bap, icaA, and mecA genes and phenotypic susceptibility to antimicrobials and teat dips},
  url          = {http://dx.doi.org/10.3168/jds.2012-5400},
  volume       = {95},
  year         = {2012},
}

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