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Effect of sampling and short isolation methodologies on the recovery of human pathogenic Yersinia enterocolitica from pig tonsils

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Abstract
The objective of this study was to determine the effect of sampling (swab samples compared to destructive samples) on isolation rates of human pathogenic Yersinia enterocolitica from pig tonsils. Moreover, the relative efficiency of different rapid, routinely applicable isolation methods was evaluated. Therefore, swab and destructive samples from tonsils of 120 pigs at slaughter were analyzed in parallel using direct plating and different enrichment methods. Salmonella-Shigella-desoxycholate-calcium chloride (SSDC) agar, cefsulodin-irgasan-novobiocin (CIN) agar, and Yersinia enterocolitica chromogenic medium (YeCM) were used as selective agar media. For enrichment, irgasan-ticarcillin-potassium chlorate (ITC) broth and peptone-sorbitol-bile (PSB) broth were incubated at 25 degrees C for 48 h. Overall, 55 tonsils (45.8%) were positive for Y. enterocolitica bioserotype 4/O:3. Recovery was significantly higher using the destructive method compared to the swabbing method. Direct plating resulted in 47 and 28 Y. enterocolitica-positive destructive and swab samples, respectively. Alkali treatment of PSB and ITC enrichment broths significantly increased recovery of pathogenic Y. enterocolitica from destructive tonsil samples. The performance of YeCM for qualitative and quantitative isolation of pathogenic Y. enterocolitica from pig tonsils was equal to SSDC and CIN. In conclusion, direct plating and ISO 10273: 2003 with minor modifications are suitable and rapid methods for isolation of pathogenic Y. enterocolitica from destructive tonsil samples.
Keywords
SLAUGHTER PIGS, POLYMERASE-CHAIN-REACTION, SOUTHERN GERMANY, PORK PRODUCTS, SEROGROUP O-3, PCR METHOD, PLASMID, FOOD, PSEUDOTUBERCULOSIS, INFECTIONS

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MLA
Van Damme, Inge, et al. “Effect of Sampling and Short Isolation Methodologies on the Recovery of Human Pathogenic Yersinia Enterocolitica from Pig Tonsils.” FOODBORNE PATHOGENS AND DISEASE, vol. 9, no. 7, 2012, pp. 600–06, doi:10.1089/fpd.2012.1128.
APA
Van Damme, I., Berkvens, D., & De Zutter, L. (2012). Effect of sampling and short isolation methodologies on the recovery of human pathogenic Yersinia enterocolitica from pig tonsils. FOODBORNE PATHOGENS AND DISEASE, 9(7), 600–606. https://doi.org/10.1089/fpd.2012.1128
Chicago author-date
Van Damme, Inge, Dirk Berkvens, and Lieven De Zutter. 2012. “Effect of Sampling and Short Isolation Methodologies on the Recovery of Human Pathogenic Yersinia Enterocolitica from Pig Tonsils.” FOODBORNE PATHOGENS AND DISEASE 9 (7): 600–606. https://doi.org/10.1089/fpd.2012.1128.
Chicago author-date (all authors)
Van Damme, Inge, Dirk Berkvens, and Lieven De Zutter. 2012. “Effect of Sampling and Short Isolation Methodologies on the Recovery of Human Pathogenic Yersinia Enterocolitica from Pig Tonsils.” FOODBORNE PATHOGENS AND DISEASE 9 (7): 600–606. doi:10.1089/fpd.2012.1128.
Vancouver
1.
Van Damme I, Berkvens D, De Zutter L. Effect of sampling and short isolation methodologies on the recovery of human pathogenic Yersinia enterocolitica from pig tonsils. FOODBORNE PATHOGENS AND DISEASE. 2012;9(7):600–6.
IEEE
[1]
I. Van Damme, D. Berkvens, and L. De Zutter, “Effect of sampling and short isolation methodologies on the recovery of human pathogenic Yersinia enterocolitica from pig tonsils,” FOODBORNE PATHOGENS AND DISEASE, vol. 9, no. 7, pp. 600–606, 2012.
@article{3097432,
  abstract     = {{The objective of this study was to determine the effect of sampling (swab samples compared to destructive samples) on isolation rates of human pathogenic Yersinia enterocolitica from pig tonsils. Moreover, the relative efficiency of different rapid, routinely applicable isolation methods was evaluated. Therefore, swab and destructive samples from tonsils of 120 pigs at slaughter were analyzed in parallel using direct plating and different enrichment methods. Salmonella-Shigella-desoxycholate-calcium chloride (SSDC) agar, cefsulodin-irgasan-novobiocin (CIN) agar, and Yersinia enterocolitica chromogenic medium (YeCM) were used as selective agar media. For enrichment, irgasan-ticarcillin-potassium chlorate (ITC) broth and peptone-sorbitol-bile (PSB) broth were incubated at 25 degrees C for 48 h. Overall, 55 tonsils (45.8%) were positive for Y. enterocolitica bioserotype 4/O:3. Recovery was significantly higher using the destructive method compared to the swabbing method. Direct plating resulted in 47 and 28 Y. enterocolitica-positive destructive and swab samples, respectively. Alkali treatment of PSB and ITC enrichment broths significantly increased recovery of pathogenic Y. enterocolitica from destructive tonsil samples. The performance of YeCM for qualitative and quantitative isolation of pathogenic Y. enterocolitica from pig tonsils was equal to SSDC and CIN. In conclusion, direct plating and ISO 10273: 2003 with minor modifications are suitable and rapid methods for isolation of pathogenic Y. enterocolitica from destructive tonsil samples.}},
  author       = {{Van Damme, Inge and Berkvens, Dirk and De Zutter, Lieven}},
  issn         = {{1535-3141}},
  journal      = {{FOODBORNE PATHOGENS AND DISEASE}},
  keywords     = {{SLAUGHTER PIGS,POLYMERASE-CHAIN-REACTION,SOUTHERN GERMANY,PORK PRODUCTS,SEROGROUP O-3,PCR METHOD,PLASMID,FOOD,PSEUDOTUBERCULOSIS,INFECTIONS}},
  language     = {{eng}},
  number       = {{7}},
  pages        = {{600--606}},
  title        = {{Effect of sampling and short isolation methodologies on the recovery of human pathogenic Yersinia enterocolitica from pig tonsils}},
  url          = {{http://doi.org/10.1089/fpd.2012.1128}},
  volume       = {{9}},
  year         = {{2012}},
}

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