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An imprinted cross-linked enzyme aggregate (iCLEA) of sucrose phosphorylase: combining improved stability with altered specificity

Karel De Winter, Wim Soetaert UGent and Tom Desmet UGent (2012) INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 13(9). p.11333-11342
abstract
The industrial use of sucrose phosphorylase (SP), an interesting biocatalyst for the selective transfer of alpha-glucosyl residues to various acceptor molecules, has been hampered by a lack of long-term stability and low activity towards alternative substrates. We have recently shown that the stability of the SP from Bifidobacterium adolescentis can be significantly improved by the formation of a cross-linked enzyme aggregate (CLEA). In this work, it is shown that the transglucosylation activity of such a CLEA can also be improved by molecular imprinting with a suitable substrate. To obtain proof of concept, SP was imprinted with alpha-glucosyl glycerol and subsequently cross-linked with glutaraldehyde. As a consequence, the enzyme's specific activity towards glycerol as acceptor substrate was increased two-fold while simultaneously providing an exceptional stability at 60 degrees C. This procedure can be performed in an aqueous environment and gives rise to a new enzyme formulation called iCLEA.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
sucrose phosphorylase, immobilization, imprinting, CLEA, glucosyl glycerol, SUBSTRATE SELECTIVITY, THERMOSTABILITY, MECHANISM, SEQUENCE, RECEPTOR, LINKING, GLUCOSE
journal title
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Int. J. Mol. Sci.
volume
13
issue
9
pages
11333 - 11342
Web of Science type
Article
Web of Science id
000309272700042
ISSN
1661-6596
DOI
10.3390/ijms130911333
project
Biotechnology for a sustainable economy (Bio-Economy)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
3097368
handle
http://hdl.handle.net/1854/LU-3097368
date created
2013-01-18 09:08:48
date last changed
2017-05-12 08:23:50
@article{3097368,
  abstract     = {The industrial use of sucrose phosphorylase (SP), an interesting biocatalyst for the selective transfer of alpha-glucosyl residues to various acceptor molecules, has been hampered by a lack of long-term stability and low activity towards alternative substrates. We have recently shown that the stability of the SP from Bifidobacterium adolescentis can be significantly improved by the formation of a cross-linked enzyme aggregate (CLEA). In this work, it is shown that the transglucosylation activity of such a CLEA can also be improved by molecular imprinting with a suitable substrate. To obtain proof of concept, SP was imprinted with alpha-glucosyl glycerol and subsequently cross-linked with glutaraldehyde. As a consequence, the enzyme's specific activity towards glycerol as acceptor substrate was increased two-fold while simultaneously providing an exceptional stability at 60 degrees C. This procedure can be performed in an aqueous environment and gives rise to a new enzyme formulation called iCLEA.},
  author       = {De Winter, Karel and Soetaert, Wim and Desmet, Tom},
  issn         = {1661-6596},
  journal      = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
  keyword      = {sucrose phosphorylase,immobilization,imprinting,CLEA,glucosyl glycerol,SUBSTRATE SELECTIVITY,THERMOSTABILITY,MECHANISM,SEQUENCE,RECEPTOR,LINKING,GLUCOSE},
  language     = {eng},
  number       = {9},
  pages        = {11333--11342},
  title        = {An imprinted cross-linked enzyme aggregate (iCLEA) of sucrose phosphorylase: combining improved stability with altered specificity},
  url          = {http://dx.doi.org/10.3390/ijms130911333},
  volume       = {13},
  year         = {2012},
}

Chicago
De Winter, Karel, Wim Soetaert, and Tom Desmet. 2012. “An Imprinted Cross-linked Enzyme Aggregate (iCLEA) of Sucrose Phosphorylase: Combining Improved Stability with Altered Specificity.” International Journal of Molecular Sciences 13 (9): 11333–11342.
APA
De Winter, K., Soetaert, W., & Desmet, T. (2012). An imprinted cross-linked enzyme aggregate (iCLEA) of sucrose phosphorylase: combining improved stability with altered specificity. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 13(9), 11333–11342.
Vancouver
1.
De Winter K, Soetaert W, Desmet T. An imprinted cross-linked enzyme aggregate (iCLEA) of sucrose phosphorylase: combining improved stability with altered specificity. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2012;13(9):11333–42.
MLA
De Winter, Karel, Wim Soetaert, and Tom Desmet. “An Imprinted Cross-linked Enzyme Aggregate (iCLEA) of Sucrose Phosphorylase: Combining Improved Stability with Altered Specificity.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 13.9 (2012): 11333–11342. Print.