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Worker honeybee sterility: a proteomic analysis of suppressed ovary activation

Dries Cardoen, Ulrich R Ernst, Bart Boerjan, Annelies Bogaerts, Ellen Formesyn, Dirk de Graaf UGent, Tom Wenseleers, Liliane Schoofs and Peter Verleyen (2012) JOURNAL OF PROTEOME RESEARCH. 11(5). p.2838-2850
abstract
Eusocial behavior is extensively studied in the honeybee, Apis mellifera, as it displays an extreme form of altruism. Honeybee workers are generally obligatory sterile in a bee colony headed by a queen, but the inhibition of ovary activation is lifted upon the absence of queen and larvae. Worker bees are then able to develop mature, viable eggs. The detailed repressive physiological mechanisms that are responsible for this remarkable phenomenon are as of yet largely unknown. Physiological studies today mainly focus on the transcriptome, while the proteome stays rather unexplored. Here, we present a quantitative 2-dimensional differential gel electrophoresis comparison between activated and inactivated worker ovaries and brains of reproductive and sterile worker bees, including a spot map of ovaries, containing 197 identified spots. Our findings suggest that suppression of ovary activation might involve a constant interplay between primordial oogenesis and subsequent degradation, which is probably mediated through steroid and neuropeptide hormone signaling. Additionally, the observation of higher viral protein loads in both the brains and ovaries of sterile workers is particularly noteworthy. This data set will be of great value for future research unraveling the physiological mechanisms underlying the altruistic sterility in honeybee workers.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
deformed wing virus, worker sterility, ovary and brain proteome, Apis mellifera, THIOREDOXIN PEROXIDASE, GEL-ELECTROPHORESIS, JUVENILE-HORMONE, DROSOPHILA-MELANOGASTER, LI-TAI-SHAO, BRAIN GENE-EXPRESSION, COCKROACH DIPLOPTERA-PUNCTATA, GENOME-WIDE ANALYSIS, BEE APIS-MELLIFERA, DEFORMED-WING-VIRUS
journal title
JOURNAL OF PROTEOME RESEARCH
J. Proteome Res.
volume
11
issue
5
pages
2838 - 2850
Web of Science type
Article
Web of Science id
000303492100018
JCR category
BIOCHEMICAL RESEARCH METHODS
JCR impact factor
5.056 (2012)
JCR rank
10/74 (2012)
JCR quartile
1 (2012)
ISSN
1535-3893
DOI
10.1021/pr201222s
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
3096459
handle
http://hdl.handle.net/1854/LU-3096459
date created
2013-01-17 14:30:03
date last changed
2016-12-19 15:39:53
@article{3096459,
  abstract     = {Eusocial behavior is extensively studied in the honeybee, Apis mellifera, as it displays an extreme form of altruism. Honeybee workers are generally obligatory sterile in a bee colony headed by a queen, but the inhibition of ovary activation is lifted upon the absence of queen and larvae. Worker bees are then able to develop mature, viable eggs. The detailed repressive physiological mechanisms that are responsible for this remarkable phenomenon are as of yet largely unknown. Physiological studies today mainly focus on the transcriptome, while the proteome stays rather unexplored. Here, we present a quantitative 2-dimensional differential gel electrophoresis comparison between activated and inactivated worker ovaries and brains of reproductive and sterile worker bees, including a spot map of ovaries, containing 197 identified spots. Our findings suggest that suppression of ovary activation might involve a constant interplay between primordial oogenesis and subsequent degradation, which is probably mediated through steroid and neuropeptide hormone signaling. Additionally, the observation of higher viral protein loads in both the brains and ovaries of sterile workers is particularly noteworthy. This data set will be of great value for future research unraveling the physiological mechanisms underlying the altruistic sterility in honeybee workers.},
  author       = {Cardoen, Dries and Ernst, Ulrich R and Boerjan, Bart and Bogaerts, Annelies and Formesyn, Ellen and de Graaf, Dirk and Wenseleers, Tom and Schoofs, Liliane and Verleyen, Peter},
  issn         = {1535-3893},
  journal      = {JOURNAL OF PROTEOME RESEARCH},
  keyword      = {deformed wing virus,worker sterility,ovary and brain proteome,Apis mellifera,THIOREDOXIN PEROXIDASE,GEL-ELECTROPHORESIS,JUVENILE-HORMONE,DROSOPHILA-MELANOGASTER,LI-TAI-SHAO,BRAIN GENE-EXPRESSION,COCKROACH DIPLOPTERA-PUNCTATA,GENOME-WIDE ANALYSIS,BEE APIS-MELLIFERA,DEFORMED-WING-VIRUS},
  language     = {eng},
  number       = {5},
  pages        = {2838--2850},
  title        = {Worker honeybee sterility: a proteomic analysis of suppressed ovary activation},
  url          = {http://dx.doi.org/10.1021/pr201222s},
  volume       = {11},
  year         = {2012},
}

Chicago
Cardoen, Dries, Ulrich R Ernst, Bart Boerjan, Annelies Bogaerts, Ellen Formesyn, Dirk de Graaf, Tom Wenseleers, Liliane Schoofs, and Peter Verleyen. 2012. “Worker Honeybee Sterility: a Proteomic Analysis of Suppressed Ovary Activation.” Journal of Proteome Research 11 (5): 2838–2850.
APA
Cardoen, Dries, Ernst, U. R., Boerjan, B., Bogaerts, A., Formesyn, E., de Graaf, D., Wenseleers, T., et al. (2012). Worker honeybee sterility: a proteomic analysis of suppressed ovary activation. JOURNAL OF PROTEOME RESEARCH, 11(5), 2838–2850.
Vancouver
1.
Cardoen D, Ernst UR, Boerjan B, Bogaerts A, Formesyn E, de Graaf D, et al. Worker honeybee sterility: a proteomic analysis of suppressed ovary activation. JOURNAL OF PROTEOME RESEARCH. 2012;11(5):2838–50.
MLA
Cardoen, Dries, Ulrich R Ernst, Bart Boerjan, et al. “Worker Honeybee Sterility: a Proteomic Analysis of Suppressed Ovary Activation.” JOURNAL OF PROTEOME RESEARCH 11.5 (2012): 2838–2850. Print.