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Worker honeybee sterility: a proteomic analysis of suppressed ovary activation

(2012) JOURNAL OF PROTEOME RESEARCH. 11(5). p.2838-2850
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Abstract
Eusocial behavior is extensively studied in the honeybee, Apis mellifera, as it displays an extreme form of altruism. Honeybee workers are generally obligatory sterile in a bee colony headed by a queen, but the inhibition of ovary activation is lifted upon the absence of queen and larvae. Worker bees are then able to develop mature, viable eggs. The detailed repressive physiological mechanisms that are responsible for this remarkable phenomenon are as of yet largely unknown. Physiological studies today mainly focus on the transcriptome, while the proteome stays rather unexplored. Here, we present a quantitative 2-dimensional differential gel electrophoresis comparison between activated and inactivated worker ovaries and brains of reproductive and sterile worker bees, including a spot map of ovaries, containing 197 identified spots. Our findings suggest that suppression of ovary activation might involve a constant interplay between primordial oogenesis and subsequent degradation, which is probably mediated through steroid and neuropeptide hormone signaling. Additionally, the observation of higher viral protein loads in both the brains and ovaries of sterile workers is particularly noteworthy. This data set will be of great value for future research unraveling the physiological mechanisms underlying the altruistic sterility in honeybee workers.
Keywords
deformed wing virus, worker sterility, ovary and brain proteome, Apis mellifera, THIOREDOXIN PEROXIDASE, GEL-ELECTROPHORESIS, JUVENILE-HORMONE, DROSOPHILA-MELANOGASTER, LI-TAI-SHAO, BRAIN GENE-EXPRESSION, COCKROACH DIPLOPTERA-PUNCTATA, GENOME-WIDE ANALYSIS, BEE APIS-MELLIFERA, DEFORMED-WING-VIRUS

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Chicago
Cardoen, Dries, Ulrich R Ernst, Bart Boerjan, Annelies Bogaerts, Ellen Formesyn, Dirk de Graaf, Tom Wenseleers, Liliane Schoofs, and Peter Verleyen. 2012. “Worker Honeybee Sterility: a Proteomic Analysis of Suppressed Ovary Activation.” Journal of Proteome Research 11 (5): 2838–2850.
APA
Cardoen, Dries, Ernst, U. R., Boerjan, B., Bogaerts, A., Formesyn, E., de Graaf, D., Wenseleers, T., et al. (2012). Worker honeybee sterility: a proteomic analysis of suppressed ovary activation. JOURNAL OF PROTEOME RESEARCH, 11(5), 2838–2850.
Vancouver
1.
Cardoen D, Ernst UR, Boerjan B, Bogaerts A, Formesyn E, de Graaf D, et al. Worker honeybee sterility: a proteomic analysis of suppressed ovary activation. JOURNAL OF PROTEOME RESEARCH. 2012;11(5):2838–50.
MLA
Cardoen, Dries, Ulrich R Ernst, Bart Boerjan, et al. “Worker Honeybee Sterility: a Proteomic Analysis of Suppressed Ovary Activation.” JOURNAL OF PROTEOME RESEARCH 11.5 (2012): 2838–2850. Print.
@article{3096459,
  abstract     = {Eusocial behavior is extensively studied in the honeybee, Apis mellifera, as it displays an extreme form of altruism. Honeybee workers are generally obligatory sterile in a bee colony headed by a queen, but the inhibition of ovary activation is lifted upon the absence of queen and larvae. Worker bees are then able to develop mature, viable eggs. The detailed repressive physiological mechanisms that are responsible for this remarkable phenomenon are as of yet largely unknown. Physiological studies today mainly focus on the transcriptome, while the proteome stays rather unexplored. Here, we present a quantitative 2-dimensional differential gel electrophoresis comparison between activated and inactivated worker ovaries and brains of reproductive and sterile worker bees, including a spot map of ovaries, containing 197 identified spots. Our findings suggest that suppression of ovary activation might involve a constant interplay between primordial oogenesis and subsequent degradation, which is probably mediated through steroid and neuropeptide hormone signaling. Additionally, the observation of higher viral protein loads in both the brains and ovaries of sterile workers is particularly noteworthy. This data set will be of great value for future research unraveling the physiological mechanisms underlying the altruistic sterility in honeybee workers.},
  author       = {Cardoen, Dries and Ernst, Ulrich R and Boerjan, Bart and Bogaerts, Annelies and Formesyn, Ellen and de Graaf, Dirk and Wenseleers, Tom and Schoofs, Liliane and Verleyen, Peter},
  issn         = {1535-3893},
  journal      = {JOURNAL OF PROTEOME RESEARCH},
  keyword      = {deformed wing virus,worker sterility,ovary and brain proteome,Apis mellifera,THIOREDOXIN PEROXIDASE,GEL-ELECTROPHORESIS,JUVENILE-HORMONE,DROSOPHILA-MELANOGASTER,LI-TAI-SHAO,BRAIN GENE-EXPRESSION,COCKROACH DIPLOPTERA-PUNCTATA,GENOME-WIDE ANALYSIS,BEE APIS-MELLIFERA,DEFORMED-WING-VIRUS},
  language     = {eng},
  number       = {5},
  pages        = {2838--2850},
  title        = {Worker honeybee sterility: a proteomic analysis of suppressed ovary activation},
  url          = {http://dx.doi.org/10.1021/pr201222s},
  volume       = {11},
  year         = {2012},
}

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