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Improvements in GC-QqQ-MS screening, 1 year of experience

Nik De Brabanter (UGent) , Wim Van Gansbeke (UGent) , Lore Geldof (UGent) and Peter Van Eenoo (UGent)
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Organization
Abstract
The previously presented screening method for doping substances using GC-MS/MS by Van Eenoo et al. has been one year in operation in our laboratory. This experience allowed us to make improvements to this method in terms of detection limits and quality control. Now the method is able to detect over 150 compounds from different classes (steroids, narcotics, stimulants, beta-2-agonists and hormone antagonists) in a qualitative way. In the quantitative part, the traditional steroid profile with most important endogenous steroids is expanded in order to improve the detection and identification of endogenous steroid misuse. Besides these also norandrosterone, salbutamol, morfine and the major metabolite of cannabis are quantified. Methods developed for anti-doping purposes should be subjected to the highest level of quality. Here, parameters to control each step in the methodology were added: hydrolysis efficiency, derivatisation efficiency and microbiological degradation are monitored in every single sample. Additionally, special attention is paid to the relation between parameters indicating degradation by micro-organisms and the reliability of the steroid profile. In this presentation, the modifications, improvements and ‘tricks’ will be presented.
Keywords
microbiological degradation, doping, Endogenous steroids, urine, GC-MS/MS, measurement uncertainty, minor metabolites

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MLA
De Brabanter, Nik, et al. “Improvements in GC-QqQ-MS Screening, 1 Year of Experience.” Recent Advances in Doping Analysis, vol. 20, Sportverslag Straus, 2012, pp. 73–73.
APA
De Brabanter, N., Van Gansbeke, W., Geldof, L., & Van Eenoo, P. (2012). Improvements in GC-QqQ-MS screening, 1 year of experience. Recent Advances in Doping Analysis, 20, 73–73. Cologne, Germany: Sportverslag Straus.
Chicago author-date
De Brabanter, Nik, Wim Van Gansbeke, Lore Geldof, and Peter Van Eenoo. 2012. “Improvements in GC-QqQ-MS Screening, 1 Year of Experience.” In Recent Advances in Doping Analysis, 20:73–73. Cologne, Germany: Sportverslag Straus.
Chicago author-date (all authors)
De Brabanter, Nik, Wim Van Gansbeke, Lore Geldof, and Peter Van Eenoo. 2012. “Improvements in GC-QqQ-MS Screening, 1 Year of Experience.” In Recent Advances in Doping Analysis, 20:73–73. Cologne, Germany: Sportverslag Straus.
Vancouver
1.
De Brabanter N, Van Gansbeke W, Geldof L, Van Eenoo P. Improvements in GC-QqQ-MS screening, 1 year of experience. In: Recent Advances in Doping Analysis. Cologne, Germany: Sportverslag Straus; 2012. p. 73–73.
IEEE
[1]
N. De Brabanter, W. Van Gansbeke, L. Geldof, and P. Van Eenoo, “Improvements in GC-QqQ-MS screening, 1 year of experience,” in Recent Advances in Doping Analysis, Köln, Germany, 2012, vol. 20, pp. 73–73.
@inproceedings{3095839,
  abstract     = {{The previously presented screening method for doping substances using GC-MS/MS by Van Eenoo et al. has been one year in operation in our laboratory. This experience allowed us to make improvements to this method in terms of detection limits and quality control.
Now the method is able to detect over 150 compounds from different classes (steroids, narcotics, stimulants, beta-2-agonists and hormone antagonists) in a qualitative way. In the quantitative part, the traditional steroid profile with most important endogenous steroids is expanded in order to improve the detection and identification of endogenous steroid misuse. Besides these also norandrosterone, salbutamol, morfine and the major metabolite of cannabis are quantified.
Methods developed for anti-doping purposes should be subjected to the highest level of quality. Here, parameters to control each step in the methodology were added: hydrolysis efficiency, derivatisation efficiency and microbiological degradation are monitored in every single sample. Additionally, special attention is paid to the relation between parameters indicating degradation by micro-organisms and the reliability of the steroid profile. 
In this presentation, the modifications, improvements and ‘tricks’ will be presented.}},
  author       = {{De Brabanter, Nik and Van Gansbeke, Wim and Geldof, Lore and Van Eenoo, Peter}},
  booktitle    = {{Recent Advances in Doping Analysis}},
  isbn         = {{9783868840384}},
  keywords     = {{microbiological degradation,doping,Endogenous steroids,urine,GC-MS/MS,measurement uncertainty,minor metabolites}},
  language     = {{eng}},
  location     = {{Köln, Germany}},
  pages        = {{73--73}},
  publisher    = {{Sportverslag Straus}},
  title        = {{Improvements in GC-QqQ-MS screening, 1 year of experience}},
  volume       = {{20}},
  year         = {{2012}},
}