Spatiotemporal regulation of lateral root organogenesis in Arabidopsis by cytokinin
- Author
- Agnieszka Bielach (UGent) , Kateřina Podlešáková, Peter Marhavy (UGent) , Jérôme Duclercq (UGent) , Candela Cuesta Moliner (UGent) , Bruno Müller, Wim Grunewald (UGent) , Petr Tarkowski and Eva Benkova (UGent)
- Organization
- Abstract
- The architecture of a plant's root system, established postembryonically, results from both coordinated root growth and lateral root branching. The plant hormones auxin and cytokinin are central endogenous signaling molecules that regulate lateral root organogenesis positively and negatively, respectively. Tight control and mutual balance of their antagonistic activities are particularly important during the early phases of lateral root organogenesis to ensure continuous lateral root initiation (LRI) and proper development of lateral root primordia (LRP). Here, we show that the early phases of lateral root organogenesis, including priming and initiation, take place in root zones with a repressed cytokinin response. Accordingly, ectopic overproduction of cytokinin in the root basal meristem most efficiently inhibits LRI. Enhanced cytokinin responses in pericycle cells between existing LRP might restrict LRI near existing LRP and, when compromised, ectopic LRI occurs. Furthermore, our results demonstrate that young LRP are more sensitive to perturbations in the cytokinin activity than are developmentally more advanced primordia. We hypothesize that the effect of cytokinin on the development of primordia possibly depends on the robustness and stability of the auxin gradient.
- Keywords
- INITIATION, MERISTEM, PLANT DEVELOPMENT, MASS-SPECTROMETRY, DEVELOPMENTAL WINDOW, FOUNDER CELLS, DEPENDENT AUXIN GRADIENTS, GENE-EXPRESSION, TRANSPORT, THALIANA
Downloads
-
Bielach et al. 2012 Plant Cell 24 3967.pdf
- full text
- |
- open access
- |
- |
- 1.25 MB
Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-3092645
- MLA
- Bielach, Agnieszka, et al. “Spatiotemporal Regulation of Lateral Root Organogenesis in Arabidopsis by Cytokinin.” PLANT CELL, vol. 24, no. 10, 2012, pp. 3967–81, doi:10.1105/tpc.112.103044.
- APA
- Bielach, A., Podlešáková, K., Marhavy, P., Duclercq, J., Cuesta Moliner, C., Müller, B., … Benkova, E. (2012). Spatiotemporal regulation of lateral root organogenesis in Arabidopsis by cytokinin. PLANT CELL, 24(10), 3967–3981. https://doi.org/10.1105/tpc.112.103044
- Chicago author-date
- Bielach, Agnieszka, Kateřina Podlešáková, Peter Marhavy, Jérôme Duclercq, Candela Cuesta Moliner, Bruno Müller, Wim Grunewald, Petr Tarkowski, and Eva Benkova. 2012. “Spatiotemporal Regulation of Lateral Root Organogenesis in Arabidopsis by Cytokinin.” PLANT CELL 24 (10): 3967–81. https://doi.org/10.1105/tpc.112.103044.
- Chicago author-date (all authors)
- Bielach, Agnieszka, Kateřina Podlešáková, Peter Marhavy, Jérôme Duclercq, Candela Cuesta Moliner, Bruno Müller, Wim Grunewald, Petr Tarkowski, and Eva Benkova. 2012. “Spatiotemporal Regulation of Lateral Root Organogenesis in Arabidopsis by Cytokinin.” PLANT CELL 24 (10): 3967–3981. doi:10.1105/tpc.112.103044.
- Vancouver
- 1.Bielach A, Podlešáková K, Marhavy P, Duclercq J, Cuesta Moliner C, Müller B, et al. Spatiotemporal regulation of lateral root organogenesis in Arabidopsis by cytokinin. PLANT CELL. 2012;24(10):3967–81.
- IEEE
- [1]A. Bielach et al., “Spatiotemporal regulation of lateral root organogenesis in Arabidopsis by cytokinin,” PLANT CELL, vol. 24, no. 10, pp. 3967–3981, 2012.
@article{3092645,
abstract = {{The architecture of a plant's root system, established postembryonically, results from both coordinated root growth and lateral root branching. The plant hormones auxin and cytokinin are central endogenous signaling molecules that regulate lateral root organogenesis positively and negatively, respectively. Tight control and mutual balance of their antagonistic activities are particularly important during the early phases of lateral root organogenesis to ensure continuous lateral root initiation (LRI) and proper development of lateral root primordia (LRP). Here, we show that the early phases of lateral root organogenesis, including priming and initiation, take place in root zones with a repressed cytokinin response. Accordingly, ectopic overproduction of cytokinin in the root basal meristem most efficiently inhibits LRI. Enhanced cytokinin responses in pericycle cells between existing LRP might restrict LRI near existing LRP and, when compromised, ectopic LRI occurs. Furthermore, our results demonstrate that young LRP are more sensitive to perturbations in the cytokinin activity than are developmentally more advanced primordia. We hypothesize that the effect of cytokinin on the development of primordia possibly depends on the robustness and stability of the auxin gradient.}},
author = {{Bielach, Agnieszka and Podlešáková, Kateřina and Marhavy, Peter and Duclercq, Jérôme and Cuesta Moliner, Candela and Müller, Bruno and Grunewald, Wim and Tarkowski, Petr and Benkova, Eva}},
issn = {{1040-4651}},
journal = {{PLANT CELL}},
keywords = {{INITIATION,MERISTEM,PLANT DEVELOPMENT,MASS-SPECTROMETRY,DEVELOPMENTAL WINDOW,FOUNDER CELLS,DEPENDENT AUXIN GRADIENTS,GENE-EXPRESSION,TRANSPORT,THALIANA}},
language = {{eng}},
number = {{10}},
pages = {{3967--3981}},
title = {{Spatiotemporal regulation of lateral root organogenesis in Arabidopsis by cytokinin}},
url = {{http://doi.org/10.1105/tpc.112.103044}},
volume = {{24}},
year = {{2012}},
}
- Altmetric
- View in Altmetric
- Web of Science
- Times cited: