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Characterization of upper lamina propria interstitial cells in bladders from patients with neurogenic detrusor overactivity and bladder pain syndrome

Thomas Gevaert, Rita De Vos, Wouter Everaerts, Louis Libbrecht, Frank Van Der Aa, Joost van den Oord, Tania Roskams and Dirk De Ridder (2011) JOURNAL OF CELLULAR AND MOLECULAR MEDICINE. 15(12). p.2586-2593
abstract
The upper lamina propria (ULP) area of interstitial cells (IC) in bladder has been studied for more than a decade in several species including human beings. Nevertheless there is still lack of uniformity in terminology of this cell layer. The aim of the present study was to add new data to the morphological and immunohistochemical phenotype of these cells and to find out whether this phenotype is changed in bladders from patients with neurogenic detrusor overactivity (NDO) and bladder pain syndrome (BPS). Bladder tissue was obtained from a control group and from patients with NDO and BPS. Samples were processed for morphology, electron microscopy and immunohistochemistry. A morphological and immunohistochemical phenotype for the ULP IC was assessed and changes in this phenotype were looked for in samples from patients with NDO and BPS. The ULP IC were characterized ultrastructurally by the presence of actin filaments with densifications, many caveolae and abundant rough endoplasmic reticulum (RER); on immunohistochemistry ULP IC were immunoreactive for a-sma, vimentin, CD10 and podoplanin and categorized as interstitial Cajal-like cells (ICLC). In NDO and BPS bladders we found a phenotypical shift towards a fibroblastic phenotype which was even more pronounced in the NDO group. In both groups there was also an increased presence in ULP lymphocytes. The ULP area in the human bladder contains a population of ICLC with distinct ultrastructural morphology and immunohistochemical phenotype. Their unique a-sma+/desmin/CD34 phenotype allows studying this population in various bladder disorders. In bladders form patients with BPS and NDO, we observed these ULP ICLC to shift towards a fibroblast phenotype.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
neurogenic detrusor overactivity, bladder, bladder pain syndrome, interstitial cell, myofibroblast, lamina propria, GUINEA-PIG BLADDER, CAJAL-LIKE CELLS, PODOPLANIN EXPRESSION, URINARY-BLADDER, SMOOTH-MUSCLE, MYOFIBROBLASTS, IDENTIFICATION, LOCALIZATION, FIBROBLASTS, CARCINOMA
journal title
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
J. Cell. Mol. Med.
volume
15
issue
12
pages
2586 - 2593
Web of Science type
Article
Web of Science id
000297855700004
JCR category
MEDICINE, RESEARCH & EXPERIMENTAL
JCR impact factor
4.125 (2011)
JCR rank
21/109 (2011)
JCR quartile
1 (2011)
ISSN
1582-1838
DOI
10.1111/j.1582-4934.2011.01262.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
3086731
handle
http://hdl.handle.net/1854/LU-3086731
date created
2013-01-09 17:24:13
date last changed
2016-12-19 15:39:07
@article{3086731,
  abstract     = {The upper lamina propria (ULP) area of interstitial cells (IC) in bladder has been studied for more than a decade in several species including human beings. Nevertheless there is still lack of uniformity in terminology of this cell layer. The aim of the present study was to add new data to the morphological and immunohistochemical phenotype of these cells and to find out whether this phenotype is changed in bladders from patients with neurogenic detrusor overactivity (NDO) and bladder pain syndrome (BPS). Bladder tissue was obtained from a control group and from patients with NDO and BPS. Samples were processed for morphology, electron microscopy and immunohistochemistry. A morphological and immunohistochemical phenotype for the ULP IC was assessed and changes in this phenotype were looked for in samples from patients with NDO and BPS. The ULP IC were characterized ultrastructurally by the presence of actin filaments with densifications, many caveolae and abundant rough endoplasmic reticulum (RER); on immunohistochemistry ULP IC were immunoreactive for a-sma, vimentin, CD10 and podoplanin and categorized as interstitial Cajal-like cells (ICLC). In NDO and BPS bladders we found a phenotypical shift towards a fibroblastic phenotype which was even more pronounced in the NDO group. In both groups there was also an increased presence in ULP lymphocytes. The ULP area in the human bladder contains a population of ICLC with distinct ultrastructural morphology and immunohistochemical phenotype. Their unique a-sma+/desmin/CD34 phenotype allows studying this population in various bladder disorders. In bladders form patients with BPS and NDO, we observed these ULP ICLC to shift towards a fibroblast phenotype.},
  author       = {Gevaert, Thomas and De Vos, Rita and Everaerts, Wouter and Libbrecht, Louis and Van Der Aa, Frank and van den Oord, Joost and Roskams, Tania and De Ridder, Dirk},
  issn         = {1582-1838},
  journal      = {JOURNAL OF CELLULAR AND MOLECULAR MEDICINE},
  keyword      = {neurogenic detrusor overactivity,bladder,bladder pain syndrome,interstitial cell,myofibroblast,lamina propria,GUINEA-PIG BLADDER,CAJAL-LIKE CELLS,PODOPLANIN EXPRESSION,URINARY-BLADDER,SMOOTH-MUSCLE,MYOFIBROBLASTS,IDENTIFICATION,LOCALIZATION,FIBROBLASTS,CARCINOMA},
  language     = {eng},
  number       = {12},
  pages        = {2586--2593},
  title        = {Characterization of upper lamina propria interstitial cells in bladders from patients with neurogenic detrusor overactivity and bladder pain syndrome},
  url          = {http://dx.doi.org/10.1111/j.1582-4934.2011.01262.x},
  volume       = {15},
  year         = {2011},
}

Chicago
Gevaert, Thomas, Rita De Vos, Wouter Everaerts, Louis Libbrecht, Frank Van Der Aa, Joost van den Oord, Tania Roskams, and Dirk De Ridder. 2011. “Characterization of Upper Lamina Propria Interstitial Cells in Bladders from Patients with Neurogenic Detrusor Overactivity and Bladder Pain Syndrome.” Journal of Cellular and Molecular Medicine 15 (12): 2586–2593.
APA
Gevaert, T., De Vos, R., Everaerts, W., Libbrecht, L., Van Der Aa, F., van den Oord, J., Roskams, T., et al. (2011). Characterization of upper lamina propria interstitial cells in bladders from patients with neurogenic detrusor overactivity and bladder pain syndrome. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 15(12), 2586–2593.
Vancouver
1.
Gevaert T, De Vos R, Everaerts W, Libbrecht L, Van Der Aa F, van den Oord J, et al. Characterization of upper lamina propria interstitial cells in bladders from patients with neurogenic detrusor overactivity and bladder pain syndrome. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE. 2011;15(12):2586–93.
MLA
Gevaert, Thomas, Rita De Vos, Wouter Everaerts, et al. “Characterization of Upper Lamina Propria Interstitial Cells in Bladders from Patients with Neurogenic Detrusor Overactivity and Bladder Pain Syndrome.” JOURNAL OF CELLULAR AND MOLECULAR MEDICINE 15.12 (2011): 2586–2593. Print.