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A new entomopathogenic nematode, Steinernema xinbinense n. sp. (Nematoda: Steinernematidae), from North China

Juan Ma, Shulong Chen, Patrick De Clercq UGent, Lieven Waeyenberge, Richou Han and Maurice Moens UGent (2012) NEMATOLOGY. 14(6). p.723-739
abstract
During a survey for entomopathogenic nematodes in northern China, a new species of Steinernema was isolated from soil samples collected from Xinbin county, Liaoning province. This nematode was obtained by the insect-baiting technique using last instar larvae of Galleria mellonella. It is described herein as S. xinbinense n. sp. The nematode can be separated from other described species of the group by morphological and morphometric characteristics of the different stages and by characterisation and phylogeny of DNA sequences of the D2D3 domain of the LSU or ITS regions of rDNA. This new species is characterised by the following morphological characters: infective third-stage juvenile with a body length of 694 (635-744) mu m, distance from head to excretory pore of 51 46-53) gm, tail length of 73 (61-81) mu m, E = 71 (65-78)%, presence of eight unevenly spaced and developed ridges in middle lateral field (i.e., nine lines). First generation male with well curved, yellowish spicules 56 (49-62) mu m long and gubernaculum 35 (30-41) mu m long, small mucron mostly present, first generation female with protruding vulva, tail conical with one or two small mucrons and D = 45 (4150)%. Cross hybridisation tests with S. tielingense, S. kraussei, S. feltiae and S. hebeiense showed that this species was reproductively isolated. The analyses of ITS-rDNA and D2D3 sequence confirm that the studied nematode isolate is a valid new species belonging to the 'feltiae-kraussei-oregonense' group.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ITS-rDNA, D2D3, molecular, morphology, morphometrics, new species, phylogeny, taxonomy, SP RHABDITIDA STEINERNEMATIDAE, PROVINCE, YUNNAN, USA
journal title
NEMATOLOGY
Nematology
volume
14
issue
6
pages
723 - 739
Web of Science type
Article
Web of Science id
000307043400005
JCR category
ZOOLOGY
JCR impact factor
0.914 (2012)
JCR rank
86/149 (2012)
JCR quartile
3 (2012)
ISSN
1388-5545
DOI
10.1163/156854112X627273
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
3082811
handle
http://hdl.handle.net/1854/LU-3082811
date created
2013-01-07 15:49:15
date last changed
2016-12-19 15:39:07
@article{3082811,
  abstract     = {During a survey for entomopathogenic nematodes in northern China, a new species of Steinernema was isolated from soil samples collected from Xinbin county, Liaoning province. This nematode was obtained by the insect-baiting technique using last instar larvae of Galleria mellonella. It is described herein as S. xinbinense n. sp. The nematode can be separated from other described species of the group by morphological and morphometric characteristics of the different stages and by characterisation and phylogeny of DNA sequences of the D2D3 domain of the LSU or ITS regions of rDNA. This new species is characterised by the following morphological characters: infective third-stage juvenile with a body length of 694 (635-744) mu m, distance from head to excretory pore of 51 46-53) gm, tail length of 73 (61-81) mu m, E = 71 (65-78)\%, presence of eight unevenly spaced and developed ridges in middle lateral field (i.e., nine lines). First generation male with well curved, yellowish spicules 56 (49-62) mu m long and gubernaculum 35 (30-41) mu m long, small mucron mostly present, first generation female with protruding vulva, tail conical with one or two small mucrons and D = 45 (4150)\%. Cross hybridisation tests with S. tielingense, S. kraussei, S. feltiae and S. hebeiense showed that this species was reproductively isolated. The analyses of ITS-rDNA and D2D3 sequence confirm that the studied nematode isolate is a valid new species belonging to the 'feltiae-kraussei-oregonense' group.},
  author       = {Ma, Juan and Chen, Shulong and De Clercq, Patrick and Waeyenberge, Lieven and Han, Richou and Moens, Maurice},
  issn         = {1388-5545},
  journal      = {NEMATOLOGY},
  keyword      = {ITS-rDNA,D2D3,molecular,morphology,morphometrics,new species,phylogeny,taxonomy,SP RHABDITIDA STEINERNEMATIDAE,PROVINCE,YUNNAN,USA},
  language     = {eng},
  number       = {6},
  pages        = {723--739},
  title        = {A new entomopathogenic nematode, Steinernema xinbinense n. sp. (Nematoda: Steinernematidae), from North China},
  url          = {http://dx.doi.org/10.1163/156854112X627273},
  volume       = {14},
  year         = {2012},
}

Chicago
Ma, Juan, Shulong Chen, Patrick De Clercq, Lieven Waeyenberge, Richou Han, and Maurice Moens. 2012. “A New Entomopathogenic Nematode, Steinernema Xinbinense N. Sp. (Nematoda: Steinernematidae), from North China.” Nematology 14 (6): 723–739.
APA
Ma, Juan, Chen, S., De Clercq, P., Waeyenberge, L., Han, R., & Moens, M. (2012). A new entomopathogenic nematode, Steinernema xinbinense n. sp. (Nematoda: Steinernematidae), from North China. NEMATOLOGY, 14(6), 723–739.
Vancouver
1.
Ma J, Chen S, De Clercq P, Waeyenberge L, Han R, Moens M. A new entomopathogenic nematode, Steinernema xinbinense n. sp. (Nematoda: Steinernematidae), from North China. NEMATOLOGY. 2012;14(6):723–39.
MLA
Ma, Juan, Shulong Chen, Patrick De Clercq, et al. “A New Entomopathogenic Nematode, Steinernema Xinbinense N. Sp. (Nematoda: Steinernematidae), from North China.” NEMATOLOGY 14.6 (2012): 723–739. Print.