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Genetic transformation of Fragaria with candidate genes involved in drought stress

(2012) Acta Horticulturae. 961. p.397-404
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Abstract
Agrobacterium-mediated transformation of Fragaria was performed in F. vesca, F. chiloensis and F. × ananassa ‘Ventana’ and ‘EL02.2011’ to allow a functional analysis of CAT (catalase), P5CS (Δ1-pyrroline-5-carboxylate synthetase) and FasAIV (sucrose acid invertase) genes under drought stress. These genes were targeted independently for the silencing by RNAi and over-expression in Fragaria. In vitro-grown plantlets of F. vesca, F. chiloensis and F. × ananassa ‘EL02.2011’ and in vivo-grown plants of F. × ananassa ‘Ventana’ were transformed with three different RNAi constructs coding for a hairpin RNA containing inverted repeat of F. vesca sequences for these three genes. They were also transformed with one construct coding for over-expression of FaAIV whole coding sequence from F. × ananassa. Transformation for all constructs was confirmed by visible GFP expression from the same T-DNA. There was no callusing and regeneration of F. chiloensis. Callus formation was obtained in F. × ananassa ‘Ventana’ but shoot proliferation was weak. F. vesca presented reasonable callusing, shoot regeneration and transformation using kanamycin (25 mg/L) selection. F. × ananassa ‘EL02.2011’ showed high regeneration and transformation efficiency with stable GFP expression in shoots. The occurrence of transgenic plants was confirmed by PCR analysis of genomic DNA in F. vesca and F. × ananassa ‘EL02.2011’. Consistent with previous observations, here direct comparison of wild species and cultivars shows that the transformation efficiency in Fragaria can be affected by the type genotype, gene, construct and explant as well as the concentration of A. tumefaciens suspension culture.
Keywords
strawberry, regeneration efficiency, Fragaria sp., Agrobacterium-mediated transformation, water deficit

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MLA
Razavi Hosseinabadi, Farzaneh, Kevin Folta, Linda Zamariola, et al. “Genetic Transformation of Fragaria with Candidate Genes Involved in Drought Stress.” Acta Horticulturae. Ed. Danny Geelen. Vol. 961. Leuven, Belgium: International Society for Horticultural Science (ISHS), 2012. 397–404. Print.
APA
Razavi Hosseinabadi, F., Folta, K., Zamariola, L., Geelen, D., De Keyzer, E., De Riek, J., & Van Labeke, M.-C. (2012). Genetic transformation of Fragaria with candidate genes involved in drought stress. In D. Geelen (Ed.), Acta Horticulturae (Vol. 961, pp. 397–404). Presented at the 7th International symposium on In Vitro Culture and Horticultural Breeding, Leuven, Belgium: International Society for Horticultural Science (ISHS).
Chicago author-date
Razavi Hosseinabadi, Farzaneh, Kevin Folta, Linda Zamariola, Danny Geelen, Ellen De Keyzer, Jan De Riek, and Marie-Christine Van Labeke. 2012. “Genetic Transformation of Fragaria with Candidate Genes Involved in Drought Stress.” In Acta Horticulturae, ed. Danny Geelen, 961:397–404. Leuven, Belgium: International Society for Horticultural Science (ISHS).
Chicago author-date (all authors)
Razavi Hosseinabadi, Farzaneh, Kevin Folta, Linda Zamariola, Danny Geelen, Ellen De Keyzer, Jan De Riek, and Marie-Christine Van Labeke. 2012. “Genetic Transformation of Fragaria with Candidate Genes Involved in Drought Stress.” In Acta Horticulturae, ed. Danny Geelen, 961:397–404. Leuven, Belgium: International Society for Horticultural Science (ISHS).
Vancouver
1.
Razavi Hosseinabadi F, Folta K, Zamariola L, Geelen D, De Keyzer E, De Riek J, et al. Genetic transformation of Fragaria with candidate genes involved in drought stress. In: Geelen D, editor. Acta Horticulturae. Leuven, Belgium: International Society for Horticultural Science (ISHS); 2012. p. 397–404.
IEEE
[1]
F. Razavi Hosseinabadi et al., “Genetic transformation of Fragaria with candidate genes involved in drought stress,” in Acta Horticulturae, Ghent, Belgium, 2012, vol. 961, pp. 397–404.
@inproceedings{3076393,
  abstract     = {Agrobacterium-mediated transformation of Fragaria was performed in F. vesca, F. chiloensis and F. × ananassa ‘Ventana’ and ‘EL02.2011’ to allow a functional analysis of CAT (catalase), P5CS (Δ1-pyrroline-5-carboxylate synthetase) and FasAIV (sucrose acid invertase) genes under drought stress. These genes were targeted independently for the silencing by RNAi and over-expression in Fragaria. In vitro-grown plantlets of F. vesca, F. chiloensis and F. × ananassa ‘EL02.2011’ and in vivo-grown plants of F. × ananassa ‘Ventana’ were transformed with three different RNAi constructs coding for a hairpin RNA containing inverted repeat of F. vesca sequences for these three genes. They were also transformed with one construct coding for over-expression of FaAIV whole coding sequence from F. × ananassa. Transformation for all constructs was confirmed by visible GFP expression from the same T-DNA. There was no callusing and regeneration of F. chiloensis. Callus formation was obtained in F. × ananassa ‘Ventana’ but shoot proliferation was weak. F. vesca presented reasonable callusing, shoot regeneration and transformation using kanamycin (25 mg/L) selection. F. × ananassa ‘EL02.2011’ showed high regeneration and transformation efficiency with stable GFP expression in shoots. The occurrence of transgenic plants was confirmed by PCR analysis of genomic DNA in F. vesca and F. × ananassa ‘EL02.2011’. Consistent with previous observations, here direct comparison of wild species and cultivars shows that the transformation efficiency in Fragaria can be affected by the type genotype, gene, construct and explant as well as the concentration of A. tumefaciens suspension culture.},
  author       = {Razavi Hosseinabadi, Farzaneh and Folta, Kevin and Zamariola, Linda and Geelen, Danny and De Keyzer, Ellen and De Riek, Jan and Van Labeke, Marie-Christine},
  booktitle    = {Acta Horticulturae},
  editor       = {Geelen, Danny},
  isbn         = {9789066055957},
  issn         = {0567-7572},
  keywords     = {strawberry,regeneration efficiency,Fragaria sp.,Agrobacterium-mediated transformation,water deficit},
  language     = {eng},
  location     = {Ghent, Belgium},
  pages        = {397--404},
  publisher    = {International Society for Horticultural Science (ISHS)},
  title        = {Genetic transformation of Fragaria with candidate genes involved in drought stress},
  url          = {http://www.actahort.org/books/961/961_52.htm},
  volume       = {961},
  year         = {2012},
}

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