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Serological profiles in nursery piglets colonized with Staphylococcus aureus

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Abstract
At present, the immune response of pigs in relation to Staphylococcus aureus carriage is poorly understood. This study aimed at investigating the dynamics of the anti-staphylococcal humoral immune response in methicillin-susceptible S. aureus (MSSA)-positive piglets and at assessing the effect of the experimental introduction of a methicillin-resistant S. aureus (MRSA) Sequence Type (ST) 398 strain. Therefore, serum samples were collected at different times from 31 weaned piglets originating from four different sows. Twenty-four out of the 31 piglets were challenged with MRSA ST398. The serum samples were analysed for IgG antibodies to 39 S. aureus antigens, using a multiplex bead-based assay (xMAP technology, Luminex Corporation). Though antibody responses showed broad inter-individual variability, serological results appeared to be clustered by litter of origin. For most antigens, an age-related response was observed with an apparent increase in antibody titres directed against staphylococcal microbial surface components recognizing adhesive matrix molecules (MSCRAMMs), which have been shown to play a role in S. aureus colonization. In most animals, antibody titres directed against staphylococcal toxins or immune-modulating proteins decreased with age, possibly reflecting absence of bacterial invasion. The introduction of MRSA ST398 did not elicit a significant humoral immune reaction. This study describes, for the first time, the humoral immune response in weaned pigs colonized with S. aureus.
Keywords
MUCOSAL IMMUNE-SYSTEM, COAGULASE-NEGATIVE STAPHYLOCOCCI, NASAL COLONIZATION, HEALTHY, GENES, IDENTIFICATION, DETERMINANTS, NONCARRIERS, CARRIAGE, ANTIGENS

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Chicago
Crombé, Florence, Wannes Vanderhaeghen, Corné P de Vogel, Willem J Van Wamel, Kurt Barbé, Katleen Hermans, Freddy Haesebrouck, and Patrick Butaye. 2013. “Serological Profiles in Nursery Piglets Colonized with Staphylococcus Aureus.” Veterinary Research 44.
APA
Crombé, F., Vanderhaeghen, W., de Vogel, C. P., Van Wamel, W. J., Barbé, K., Hermans, K., Haesebrouck, F., et al. (2013). Serological profiles in nursery piglets colonized with Staphylococcus aureus. VETERINARY RESEARCH, 44.
Vancouver
1.
Crombé F, Vanderhaeghen W, de Vogel CP, Van Wamel WJ, Barbé K, Hermans K, et al. Serological profiles in nursery piglets colonized with Staphylococcus aureus. VETERINARY RESEARCH. 2013;44.
MLA
Crombé, Florence, Wannes Vanderhaeghen, Corné P de Vogel, et al. “Serological Profiles in Nursery Piglets Colonized with Staphylococcus Aureus.” VETERINARY RESEARCH 44 (2013): n. pag. Print.
@article{3063389,
  abstract     = {At present, the immune response of pigs in relation to Staphylococcus aureus carriage is poorly understood. This study aimed at investigating the dynamics of the anti-staphylococcal humoral immune response in methicillin-susceptible S. aureus (MSSA)-positive piglets and at assessing the effect of the experimental introduction of a methicillin-resistant S. aureus (MRSA) Sequence Type (ST) 398 strain. Therefore, serum samples were collected at different times from 31 weaned piglets originating from four different sows. Twenty-four out of the 31 piglets were challenged with MRSA ST398. The serum samples were analysed for IgG antibodies to 39 S. aureus antigens, using a multiplex bead-based assay (xMAP technology, Luminex Corporation). Though antibody responses showed broad inter-individual variability, serological results appeared to be clustered by litter of origin. For most antigens, an age-related response was observed with an apparent increase in antibody titres directed against staphylococcal microbial surface components recognizing adhesive matrix molecules (MSCRAMMs), which have been shown to play a role in S. aureus colonization. In most animals, antibody titres directed against staphylococcal toxins or immune-modulating proteins decreased with age, possibly reflecting absence of bacterial invasion. The introduction of MRSA ST398 did not elicit a significant humoral immune reaction.
This study describes, for the first time, the humoral immune response in weaned pigs colonized with S. aureus.},
  articleno    = {4},
  author       = {Cromb{\'e}, Florence and Vanderhaeghen, Wannes and de Vogel, Corn{\'e} P and Van Wamel, Willem J and Barb{\'e}, Kurt and Hermans, Katleen and Haesebrouck, Freddy and Butaye, Patrick},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  language     = {eng},
  pages        = {10},
  title        = {Serological profiles in nursery piglets colonized with Staphylococcus aureus},
  url          = {http://dx.doi.org/10.1186/1297-9716-44-4},
  volume       = {44},
  year         = {2013},
}

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