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Inactivation of Bacillus cereus vegetative cells by gastric acid and bile during in vitro gastrointestinal transit

Siele Ceuppens UGent, Mieke Uyttendaele UGent, Stefanie Hamelink UGent, Nico Boon UGent and Tom Van de Wiele UGent (2012) GUT PATHOGENS. 4.
abstract
Background: The foodborne pathogen Bacillus cereus can cause diarrhoeal food poisoning by production of enterotoxins in the small intestine. The prerequisite for diarrhoeal disease is thus survival during gastrointestinal passage. Methods: Vegetative cells of 3 different B. cereus strains were cultivated in a real composite food matrix, lasagne verde, and their survival during subsequent simulation of gastrointestinal passage was assessed using in vitro experiments simulating transit through the human upper gastrointestinal tract (from mouth to small intestine). Results: No survival of vegetative cells was observed, despite the high inoculum levels of 7.0 to 8.0 log CFU/g and the presence of various potentially protective food components. Significant fractions (approx. 10 % of the consumed inoculum) of B. cereus vegetative cells survived gastric passage, but they were subsequently inactivated by bile exposure in weakly acidic intestinal medium (pH 5.0). In contrast, the low numbers of spores present (up to 4.0 log spores/g) showed excellent survival and remained viable spores throughout the gastrointestinal passage simulation. Conclusion: Vegetative cells are inactivated by gastric acid and bile during gastrointestinal passage, while spores are resistant and survive. Therefore, the physiological form (vegetative cells or spores) of the B. cereus consumed determines the subsequent gastrointestinal survival and thus the infective dose, which is expected to be much lower for spores than vegetative cells. No significant differences in gastrointestinal survival ability was found among the different strains. However, considerable strain variability was observed in sporulation tendency during growth in laboratory medium and food, which has important implications for the gastrointestinal survival potential of the different B. cereus strains.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Bacillus cereus, Bile, In vitro simulation, Gastrointestinal passage, HUMAN SMALL-INTESTINE, PROTON PERMEABILITY, STRESS RESPONSES, SURVIVAL, RAT, HEPATOCYTES, CONJUGATION, RESISTANCE, BACTERIA, LISTERIA
journal title
GUT PATHOGENS
Gut Pathogens
volume
4
article_number
11
pages
7 pages
Web of Science type
Article
Web of Science id
000311827400001
JCR category
MICROBIOLOGY
JCR impact factor
2.738 (2012)
JCR rank
48/116 (2012)
JCR quartile
2 (2012)
ISSN
1757-4749
DOI
10.1186/1757-4749-4-11
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
3063167
handle
http://hdl.handle.net/1854/LU-3063167
date created
2012-11-30 09:36:42
date last changed
2015-06-17 10:19:21
@article{3063167,
  abstract     = {Background: The foodborne pathogen Bacillus cereus can cause diarrhoeal food poisoning by production of enterotoxins in the small intestine. The prerequisite for diarrhoeal disease is thus survival during gastrointestinal passage. 
Methods: Vegetative cells of 3 different B. cereus strains were cultivated in a real composite food matrix, lasagne verde, and their survival during subsequent simulation of gastrointestinal passage was assessed using in vitro experiments simulating transit through the human upper gastrointestinal tract (from mouth to small intestine). 
Results: No survival of vegetative cells was observed, despite the high inoculum levels of 7.0 to 8.0 log CFU/g and the presence of various potentially protective food components. Significant fractions (approx. 10 \% of the consumed inoculum) of B. cereus vegetative cells survived gastric passage, but they were subsequently inactivated by bile exposure in weakly acidic intestinal medium (pH 5.0). In contrast, the low numbers of spores present (up to 4.0 log spores/g) showed excellent survival and remained viable spores throughout the gastrointestinal passage simulation. 
Conclusion: Vegetative cells are inactivated by gastric acid and bile during gastrointestinal passage, while spores are resistant and survive. Therefore, the physiological form (vegetative cells or spores) of the B. cereus consumed determines the subsequent gastrointestinal survival and thus the infective dose, which is expected to be much lower for spores than vegetative cells. No significant differences in gastrointestinal survival ability was found among the different strains. However, considerable strain variability was observed in sporulation tendency during growth in laboratory medium and food, which has important implications for the gastrointestinal survival potential of the different B. cereus strains.},
  articleno    = {11},
  author       = {Ceuppens, Siele and Uyttendaele, Mieke and Hamelink, Stefanie and Boon, Nico and Van de Wiele, Tom},
  issn         = {1757-4749},
  journal      = {GUT PATHOGENS},
  keyword      = {Bacillus cereus,Bile,In vitro simulation,Gastrointestinal passage,HUMAN SMALL-INTESTINE,PROTON PERMEABILITY,STRESS RESPONSES,SURVIVAL,RAT,HEPATOCYTES,CONJUGATION,RESISTANCE,BACTERIA,LISTERIA},
  language     = {eng},
  pages        = {7},
  title        = {Inactivation of Bacillus cereus vegetative cells by gastric acid and bile during in vitro gastrointestinal transit},
  url          = {http://dx.doi.org/10.1186/1757-4749-4-11},
  volume       = {4},
  year         = {2012},
}

Chicago
Ceuppens, Siele, Mieke Uyttendaele, Stefanie Hamelink, Nico Boon, and Tom Van de Wiele. 2012. “Inactivation of Bacillus Cereus Vegetative Cells by Gastric Acid and Bile During in Vitro Gastrointestinal Transit.” Gut Pathogens 4.
APA
Ceuppens, S., Uyttendaele, M., Hamelink, S., Boon, N., & Van de Wiele, T. (2012). Inactivation of Bacillus cereus vegetative cells by gastric acid and bile during in vitro gastrointestinal transit. GUT PATHOGENS, 4.
Vancouver
1.
Ceuppens S, Uyttendaele M, Hamelink S, Boon N, Van de Wiele T. Inactivation of Bacillus cereus vegetative cells by gastric acid and bile during in vitro gastrointestinal transit. GUT PATHOGENS. 2012;4.
MLA
Ceuppens, Siele, Mieke Uyttendaele, Stefanie Hamelink, et al. “Inactivation of Bacillus Cereus Vegetative Cells by Gastric Acid and Bile During in Vitro Gastrointestinal Transit.” GUT PATHOGENS 4 (2012): n. pag. Print.