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Histone H2B monoubiquitination is required to reach maximal transcript levels of circadian clock genes in Arabidopsis

Kristiina Himanen UGent, Magdalena Woloszynska UGent, Tommaso M Boccardi, Steven De Groeve UGent, Hilde Nelissen UGent, Leonardo Bruno, Marnik Vuylsteke UGent and Maria Van Lijsebettens UGent (2012) PLANT JOURNAL. 72(2). p.249-260
abstract
Previously, we identified HISTONE MONOUBIQUITINATION1 (HUB1) as an unconventional ubiquitin E3 ligase that is not involved in protein degradation but in the histone H2B modification that is implicated in transcriptional activation in plants. HUB1-mediated regulation of gene expression played a role in periodic and inducible processes such as the cell cycle, dormancy, flowering time and defense responses. Here, we determined the effects of the hub1-1 mutation on expression of a set of diurnally induced circadian clock genes identified from a comparative microarray analysis between the hub1-1 mutant and an HUB1 over-expression line. The hub1-1 mutation reduced the amplitudes of a number of induced clock gene expression peaks, as well as the HUB1-mediated histone H2BUb and H3K4Me3 marks associated with the coding regions, suggesting a role for HUB1 in facilitating transcriptional elongation in plants. Furthermore, double mutants between hub1-1 and elongata (elo) showed an embryo-lethal phenotype, indicating a synergistic genetic interaction. The double mutant embryos arrested at the torpedo stage, implying that together histone ubiquitination and acetylation marks are essential to activate expression of target genes in multiple pathways.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Arabidopsis, IN-SITU HYBRIDIZATION, transcriptional elongation, histone H3K4 trimethylation, histone H2B monoubiquitination, circadian clock, chromatin, RNA-POLYMERASE-II, FLOWERING-LOCUS-C, SEED DORMANCY, GROWTH, UBIQUITYLATION, ELONGATION, CHROMATIN, THALIANA, RHYTHMS
journal title
PLANT JOURNAL
Plant J.
volume
72
issue
2
pages
249 - 260
Web of Science type
Article
Web of Science id
000309910300008
JCR category
PLANT SCIENCES
JCR impact factor
6.582 (2012)
JCR rank
7/193 (2012)
JCR quartile
1 (2012)
ISSN
0960-7412
DOI
10.1111/j.1365-313X.2012.05071.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
3062551
handle
http://hdl.handle.net/1854/LU-3062551
date created
2012-11-29 10:57:40
date last changed
2012-11-30 09:34:54
@article{3062551,
  abstract     = {Previously, we identified HISTONE MONOUBIQUITINATION1 (HUB1) as an unconventional ubiquitin E3 ligase that is not involved in protein degradation but in the histone H2B modification that is implicated in transcriptional activation in plants. HUB1-mediated regulation of gene expression played a role in periodic and inducible processes such as the cell cycle, dormancy, flowering time and defense responses. Here, we determined the effects of the hub1-1 mutation on expression of a set of diurnally induced circadian clock genes identified from a comparative microarray analysis between the hub1-1 mutant and an HUB1 over-expression line. The hub1-1 mutation reduced the amplitudes of a number of induced clock gene expression peaks, as well as the HUB1-mediated histone H2BUb and H3K4Me3 marks associated with the coding regions, suggesting a role for HUB1 in facilitating transcriptional elongation in plants. Furthermore, double mutants between hub1-1 and elongata (elo) showed an embryo-lethal phenotype, indicating a synergistic genetic interaction. The double mutant embryos arrested at the torpedo stage, implying that together histone ubiquitination and acetylation marks are essential to activate expression of target genes in multiple pathways.},
  author       = {Himanen, Kristiina and Woloszynska, Magdalena and Boccardi, Tommaso M and De Groeve, Steven and Nelissen, Hilde and Bruno, Leonardo and Vuylsteke, Marnik and Van Lijsebettens, Maria},
  issn         = {0960-7412},
  journal      = {PLANT JOURNAL},
  keyword      = {Arabidopsis,IN-SITU HYBRIDIZATION,transcriptional elongation,histone H3K4 trimethylation,histone H2B monoubiquitination,circadian clock,chromatin,RNA-POLYMERASE-II,FLOWERING-LOCUS-C,SEED DORMANCY,GROWTH,UBIQUITYLATION,ELONGATION,CHROMATIN,THALIANA,RHYTHMS},
  language     = {eng},
  number       = {2},
  pages        = {249--260},
  title        = {Histone H2B monoubiquitination is required to reach maximal transcript levels of circadian clock genes in Arabidopsis},
  url          = {http://dx.doi.org/10.1111/j.1365-313X.2012.05071.x},
  volume       = {72},
  year         = {2012},
}

Chicago
Himanen, Kristiina, Magdalena Woloszynska, Tommaso M Boccardi, Steven De Groeve, Hilde Nelissen, Leonardo Bruno, Marnik Vuylsteke, and Maria Van Lijsebettens. 2012. “Histone H2B Monoubiquitination Is Required to Reach Maximal Transcript Levels of Circadian Clock Genes in Arabidopsis.” Plant Journal 72 (2): 249–260.
APA
Himanen, K., Woloszynska, M., Boccardi, T. M., De Groeve, S., Nelissen, H., Bruno, L., Vuylsteke, M., et al. (2012). Histone H2B monoubiquitination is required to reach maximal transcript levels of circadian clock genes in Arabidopsis. PLANT JOURNAL, 72(2), 249–260.
Vancouver
1.
Himanen K, Woloszynska M, Boccardi TM, De Groeve S, Nelissen H, Bruno L, et al. Histone H2B monoubiquitination is required to reach maximal transcript levels of circadian clock genes in Arabidopsis. PLANT JOURNAL. 2012;72(2):249–60.
MLA
Himanen, Kristiina, Magdalena Woloszynska, Tommaso M Boccardi, et al. “Histone H2B Monoubiquitination Is Required to Reach Maximal Transcript Levels of Circadian Clock Genes in Arabidopsis.” PLANT JOURNAL 72.2 (2012): 249–260. Print.