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A transgenic mouse marking live replicating cells reveals in vivo transcriptional program of proliferation

(2012) DEVELOPMENTAL CELL. 23(4). p.681-690
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Abstract
Most adult mammalian tissues are quiescent, with rare cell divisions serving to maintain homeostasis. At present, the isolation and study of replicating cells from their in vivo niche typically involves immunostaining for intracellular markers of proliferation, causing the loss of sensitive biological material. We describe a transgenic mouse strain, expressing a CyclinB1-GFP fusion reporter, that marks replicating cells in the S/G2/M phases of the cell cycle. Using flow cytometry, we isolate live replicating cells from the liver and compare their transcriptome to that of quiescent cells to reveal gene expression programs associated with cell proliferation in vivo. We find that replicating hepatocytes have reduced expression of genes characteristic of liver differentiation. This reporter system provides a powerful platform for gene expression and metabolic and functional studies of replicating cells in their in vivo niche.
Keywords
DESTRUCTION, REGENERATION, BETA-CELL, GENE-EXPRESSION, CYCLE PROGRESSION, EXPRESSION PROFILES, HEPATOCELLULAR-CARCINOMA, HEPATIC PROGENITOR CELLS, LOCALIZATION, HEPATOCYTES

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MLA
Klochendler, Agnes, et al. “A Transgenic Mouse Marking Live Replicating Cells Reveals in Vivo Transcriptional Program of Proliferation.” DEVELOPMENTAL CELL, vol. 23, no. 4, 2012, pp. 681–90, doi:10.1016/j.devcel.2012.08.009.
APA
Klochendler, A., Weinberg-Corem, N., Moran, M., Swisa, A., Pochet, N., Savova, V., … Eden, A. (2012). A transgenic mouse marking live replicating cells reveals in vivo transcriptional program of proliferation. DEVELOPMENTAL CELL, 23(4), 681–690. https://doi.org/10.1016/j.devcel.2012.08.009
Chicago author-date
Klochendler, Agnes, Noa Weinberg-Corem, Maya Moran, Avital Swisa, Nathalie Pochet, Virginia Savova, Jonas Vikeså, et al. 2012. “A Transgenic Mouse Marking Live Replicating Cells Reveals in Vivo Transcriptional Program of Proliferation.” DEVELOPMENTAL CELL 23 (4): 681–90. https://doi.org/10.1016/j.devcel.2012.08.009.
Chicago author-date (all authors)
Klochendler, Agnes, Noa Weinberg-Corem, Maya Moran, Avital Swisa, Nathalie Pochet, Virginia Savova, Jonas Vikeså, Yves Van de Peer, Michael Brandeis, Aviv Regev, Finn Cilius Nielsen, Yuval Dor, and Amir Eden. 2012. “A Transgenic Mouse Marking Live Replicating Cells Reveals in Vivo Transcriptional Program of Proliferation.” DEVELOPMENTAL CELL 23 (4): 681–690. doi:10.1016/j.devcel.2012.08.009.
Vancouver
1.
Klochendler A, Weinberg-Corem N, Moran M, Swisa A, Pochet N, Savova V, et al. A transgenic mouse marking live replicating cells reveals in vivo transcriptional program of proliferation. DEVELOPMENTAL CELL. 2012;23(4):681–90.
IEEE
[1]
A. Klochendler et al., “A transgenic mouse marking live replicating cells reveals in vivo transcriptional program of proliferation,” DEVELOPMENTAL CELL, vol. 23, no. 4, pp. 681–690, 2012.
@article{3059318,
  abstract     = {{Most adult mammalian tissues are quiescent, with rare cell divisions serving to maintain homeostasis. At present, the isolation and study of replicating cells from their in vivo niche typically involves immunostaining for intracellular markers of proliferation, causing the loss of sensitive biological material. We describe a transgenic mouse strain, expressing a CyclinB1-GFP fusion reporter, that marks replicating cells in the S/G2/M phases of the cell cycle. Using flow cytometry, we isolate live replicating cells from the liver and compare their transcriptome to that of quiescent cells to reveal gene expression programs associated with cell proliferation in vivo. We find that replicating hepatocytes have reduced expression of genes characteristic of liver differentiation. This reporter system provides a powerful platform for gene expression and metabolic and functional studies of replicating cells in their in vivo niche.}},
  author       = {{Klochendler, Agnes and Weinberg-Corem, Noa and Moran, Maya and Swisa, Avital and Pochet, Nathalie and Savova, Virginia and Vikeså, Jonas and Van de Peer, Yves and Brandeis, Michael and Regev, Aviv and Nielsen, Finn Cilius and Dor, Yuval and Eden, Amir}},
  issn         = {{1534-5807}},
  journal      = {{DEVELOPMENTAL CELL}},
  keywords     = {{DESTRUCTION,REGENERATION,BETA-CELL,GENE-EXPRESSION,CYCLE PROGRESSION,EXPRESSION PROFILES,HEPATOCELLULAR-CARCINOMA,HEPATIC PROGENITOR CELLS,LOCALIZATION,HEPATOCYTES}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{681--690}},
  title        = {{A transgenic mouse marking live replicating cells reveals in vivo transcriptional program of proliferation}},
  url          = {{http://doi.org/10.1016/j.devcel.2012.08.009}},
  volume       = {{23}},
  year         = {{2012}},
}

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