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CCS52 and DEL1 genes are key components of the endocycle in nematode-induced feeding sites

(2012) PLANT JOURNAL. 72(2). p.185-198
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Abstract
The establishment of galls and syncytia as feeding sites induced by root-knot and cyst nematodes, respectively, involves a progressive increase in nuclear and cellular size. Here we describe the functional characterization of endocycle activators CCS52A, CCS52B and a repressor of the endocycle, DEL1, during two types of nematode feeding site development in Arabidopsis thaliana. In situ hybridization analysis showed that expression of CCS52A1 and CCS52B was strongly induced in galls and syncytia and DEL1 was stably but weakly expressed throughout feeding site development. Down-regulation and over-expression of CCS52 and DEL1 in Arabidopsis drastically affected giant cell and syncytium growth, resulting in restrained nematode development, illustrating the need for mitotic activity and endo-reduplication for feeding site maturation. Exploiting the mechanism of endo-reduplication may be envisaged as a strategy to control plant-parasitic nematodes.
Keywords
Arabidopsis, MEDICAGO-TRUNCATULA, ARABIDOPSIS-THALIANA, endocycle, cell cycle, root-knot nematode, cyst nematode, ANAPHASE-PROMOTING COMPLEX, PARASITIC NEMATODE, DNA TOPOISOMERASE-VI, CELL-CYCLE, MELOIDOGYNE-INCOGNITA, ROOT-KNOT, FRUIT-DEVELOPMENT, GIANT-CELLS

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Citation

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Chicago
de Almeida Engler, Janice, Tina Kyndt, Paulo Vieira, Elke Van Cappelle, Véronique Boudolf, Vanesa Sanchez, Carolina Escobar, et al. 2012. “CCS52 and DEL1 Genes Are Key Components of the Endocycle in Nematode-induced Feeding Sites.” Plant Journal 72 (2): 185–198.
APA
de Almeida Engler, J., Kyndt, T., Vieira, P., Van Cappelle, E., Boudolf, V., Sanchez, V., Escobar, C., et al. (2012). CCS52 and DEL1 genes are key components of the endocycle in nematode-induced feeding sites. PLANT JOURNAL, 72(2), 185–198.
Vancouver
1.
de Almeida Engler J, Kyndt T, Vieira P, Van Cappelle E, Boudolf V, Sanchez V, et al. CCS52 and DEL1 genes are key components of the endocycle in nematode-induced feeding sites. PLANT JOURNAL. 2012;72(2):185–98.
MLA
de Almeida Engler, Janice, Tina Kyndt, Paulo Vieira, et al. “CCS52 and DEL1 Genes Are Key Components of the Endocycle in Nematode-induced Feeding Sites.” PLANT JOURNAL 72.2 (2012): 185–198. Print.
@article{3059261,
  abstract     = {The establishment of galls and syncytia as feeding sites induced by root-knot and cyst nematodes, respectively, involves a progressive increase in nuclear and cellular size. Here we describe the functional characterization of endocycle activators CCS52A, CCS52B and a repressor of the endocycle, DEL1, during two types of nematode feeding site development in Arabidopsis thaliana. In situ hybridization analysis showed that expression of CCS52A1 and CCS52B was strongly induced in galls and syncytia and DEL1 was stably but weakly expressed throughout feeding site development. Down-regulation and over-expression of CCS52 and DEL1 in Arabidopsis drastically affected giant cell and syncytium growth, resulting in restrained nematode development, illustrating the need for mitotic activity and endo-reduplication for feeding site maturation. Exploiting the mechanism of endo-reduplication may be envisaged as a strategy to control plant-parasitic nematodes.},
  author       = {de Almeida Engler, Janice and Kyndt, Tina and Vieira, Paulo and Van Cappelle, Elke and Boudolf, V{\'e}ronique and Sanchez, Vanesa and Escobar, Carolina and De Veylder, Lieven and Engler, Gilbert and Abad, Pierre and Gheysen, Godelieve},
  issn         = {0960-7412},
  journal      = {PLANT JOURNAL},
  keyword      = {Arabidopsis,MEDICAGO-TRUNCATULA,ARABIDOPSIS-THALIANA,endocycle,cell cycle,root-knot nematode,cyst nematode,ANAPHASE-PROMOTING COMPLEX,PARASITIC NEMATODE,DNA TOPOISOMERASE-VI,CELL-CYCLE,MELOIDOGYNE-INCOGNITA,ROOT-KNOT,FRUIT-DEVELOPMENT,GIANT-CELLS},
  language     = {eng},
  number       = {2},
  pages        = {185--198},
  title        = {CCS52 and DEL1 genes are key components of the endocycle in nematode-induced feeding sites},
  url          = {http://dx.doi.org/10.1111/j.1365-313X.2012.05054.x},
  volume       = {72},
  year         = {2012},
}

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