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CCS52 and DEL1 genes are key components of the endocycle in nematode-induced feeding sites

Janice de Almeida Engler, Tina Kyndt UGent, Paulo Vieira, Elke Van Cappelle, Véronique Boudolf UGent, Vanesa Sanchez, Carolina Escobar, Lieven De Veylder UGent, Gilbert Engler UGent, Pierre Abad, et al. (2012) PLANT JOURNAL. 72(2). p.185-198
abstract
The establishment of galls and syncytia as feeding sites induced by root-knot and cyst nematodes, respectively, involves a progressive increase in nuclear and cellular size. Here we describe the functional characterization of endocycle activators CCS52A, CCS52B and a repressor of the endocycle, DEL1, during two types of nematode feeding site development in Arabidopsis thaliana. In situ hybridization analysis showed that expression of CCS52A1 and CCS52B was strongly induced in galls and syncytia and DEL1 was stably but weakly expressed throughout feeding site development. Down-regulation and over-expression of CCS52 and DEL1 in Arabidopsis drastically affected giant cell and syncytium growth, resulting in restrained nematode development, illustrating the need for mitotic activity and endo-reduplication for feeding site maturation. Exploiting the mechanism of endo-reduplication may be envisaged as a strategy to control plant-parasitic nematodes.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Arabidopsis, MEDICAGO-TRUNCATULA, ARABIDOPSIS-THALIANA, endocycle, cell cycle, root-knot nematode, cyst nematode, ANAPHASE-PROMOTING COMPLEX, PARASITIC NEMATODE, DNA TOPOISOMERASE-VI, CELL-CYCLE, MELOIDOGYNE-INCOGNITA, ROOT-KNOT, FRUIT-DEVELOPMENT, GIANT-CELLS
journal title
PLANT JOURNAL
Plant J.
volume
72
issue
2
pages
185 - 198
Web of Science type
Article
Web of Science id
000309910300003
JCR category
PLANT SCIENCES
JCR impact factor
6.582 (2012)
JCR rank
7/193 (2012)
JCR quartile
1 (2012)
ISSN
0960-7412
DOI
10.1111/j.1365-313X.2012.05054.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
3059261
handle
http://hdl.handle.net/1854/LU-3059261
date created
2012-11-23 15:26:57
date last changed
2016-12-19 15:43:35
@article{3059261,
  abstract     = {The establishment of galls and syncytia as feeding sites induced by root-knot and cyst nematodes, respectively, involves a progressive increase in nuclear and cellular size. Here we describe the functional characterization of endocycle activators CCS52A, CCS52B and a repressor of the endocycle, DEL1, during two types of nematode feeding site development in Arabidopsis thaliana. In situ hybridization analysis showed that expression of CCS52A1 and CCS52B was strongly induced in galls and syncytia and DEL1 was stably but weakly expressed throughout feeding site development. Down-regulation and over-expression of CCS52 and DEL1 in Arabidopsis drastically affected giant cell and syncytium growth, resulting in restrained nematode development, illustrating the need for mitotic activity and endo-reduplication for feeding site maturation. Exploiting the mechanism of endo-reduplication may be envisaged as a strategy to control plant-parasitic nematodes.},
  author       = {de Almeida Engler, Janice and Kyndt, Tina and Vieira, Paulo and Van Cappelle, Elke and Boudolf, V{\'e}ronique and Sanchez, Vanesa and Escobar, Carolina and De Veylder, Lieven and Engler, Gilbert and Abad, Pierre and Gheysen, Godelieve},
  issn         = {0960-7412},
  journal      = {PLANT JOURNAL},
  keyword      = {Arabidopsis,MEDICAGO-TRUNCATULA,ARABIDOPSIS-THALIANA,endocycle,cell cycle,root-knot nematode,cyst nematode,ANAPHASE-PROMOTING COMPLEX,PARASITIC NEMATODE,DNA TOPOISOMERASE-VI,CELL-CYCLE,MELOIDOGYNE-INCOGNITA,ROOT-KNOT,FRUIT-DEVELOPMENT,GIANT-CELLS},
  language     = {eng},
  number       = {2},
  pages        = {185--198},
  title        = {CCS52 and DEL1 genes are key components of the endocycle in nematode-induced feeding sites},
  url          = {http://dx.doi.org/10.1111/j.1365-313X.2012.05054.x},
  volume       = {72},
  year         = {2012},
}

Chicago
de Almeida Engler, Janice, Tina Kyndt, Paulo Vieira, Elke Van Cappelle, Véronique Boudolf, Vanesa Sanchez, Carolina Escobar, et al. 2012. “CCS52 and DEL1 Genes Are Key Components of the Endocycle in Nematode-induced Feeding Sites.” Plant Journal 72 (2): 185–198.
APA
de Almeida Engler, J., Kyndt, T., Vieira, P., Van Cappelle, E., Boudolf, V., Sanchez, V., Escobar, C., et al. (2012). CCS52 and DEL1 genes are key components of the endocycle in nematode-induced feeding sites. PLANT JOURNAL, 72(2), 185–198.
Vancouver
1.
de Almeida Engler J, Kyndt T, Vieira P, Van Cappelle E, Boudolf V, Sanchez V, et al. CCS52 and DEL1 genes are key components of the endocycle in nematode-induced feeding sites. PLANT JOURNAL. 2012;72(2):185–98.
MLA
de Almeida Engler, Janice, Tina Kyndt, Paulo Vieira, et al. “CCS52 and DEL1 Genes Are Key Components of the Endocycle in Nematode-induced Feeding Sites.” PLANT JOURNAL 72.2 (2012): 185–198. Print.