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Electrophysiological responses to vagus nerve stimulation in rats

Lies Mollet UGent, Robrecht Raedt UGent, RIEM EL TAHRY UGent, Jean Delbeke UGent, Veerle De Herdt UGent, Alfred Meurs UGent, Wytse Wadman UGent, Kristl Vonck UGent and Paul Boon UGent (2011) EPILEPSIA. 52(suppl. 6). p.160-160
abstract
Vagus nerve stimulation (VNS) for refractory epilepsy requires optimization of stimulation parameters to improve outcome. Measuring electrophysiological activity from the vagus nerve in response to electrical stimulation may provide an objective tool to evaluate the effects of various stimulation parameters in an experimental set-up. Rats were implanted with a stimulation electrode around the left cervical vagus nerve. Electrophysiological recordings were performed using thin point electrodes placed on the vagus nerve 2 and 4mm rostrally to the cathode. Reference/ground electrode was placed in the wound. The vagus nerve was stimulated with a biphasic, charge-balanced pulse. Silk wire was strapped along the vagus nerve to cause reversible lesions of the nerve. VNS induced an electrophysiological response consisting of a fast and a slow component. The threshold intensity was 2490±240µA and 2067±247µA respectively. The components reached their maximum amplitude at 3875±530µA and 3000±935 µA. Mean latency, at 2mm, was 0.4±0.1ms and 2.6±0.3ms. Conduction velocity for the fast component was 25m/s. The fast component disappeared by afferent lesioning the vagus nerve. The slow component disappeared by efferent lesioning, by lesioning the recurrent laryngeal nerve and by applying Vecuronium to the larynx muscles. A short, single electrical pulse activates fast conducting afferent fibers. Also efferent fibers of the recurrent laryngeal nerve are activated resulting in contraction of larynx muscles. A far field potential was recorded on the vagus nerve. Our set-up can be used to evaluate the effect of stimulation parameters at the cervical vagus nerve in rat epilepsy models.
Please use this url to cite or link to this publication:
author
organization
year
type
conference
publication status
published
subject
in
EPILEPSIA
Epilepsia
volume
52
issue
suppl. 6
pages
160 - 160
conference name
29th International Epilepsy Congress
conference location
Rome, Italy
conference start
2011-08-28
conference end
2011-09-01
Web of Science type
Meeting Abstract
Web of Science id
000294217200517
JCR category
CLINICAL NEUROLOGY
JCR impact factor
3.961 (2011)
JCR rank
31/190 (2011)
JCR quartile
1 (2011)
ISSN
0013-9580
language
English
UGent publication?
yes
classification
C3
id
3051398
handle
http://hdl.handle.net/1854/LU-3051398
date created
2012-11-12 16:56:20
date last changed
2012-11-13 10:04:00
@inproceedings{3051398,
  abstract     = {Vagus nerve stimulation (VNS) for refractory epilepsy requires optimization of stimulation parameters to improve outcome. Measuring electrophysiological activity from the vagus nerve in response to electrical stimulation may provide an objective tool to evaluate the effects of various stimulation parameters in an experimental set-up.
Rats were implanted with a stimulation electrode around the left cervical vagus nerve. Electrophysiological recordings were performed using thin point electrodes placed on the vagus nerve 2 and 4mm rostrally to the cathode. Reference/ground electrode was placed in the wound. The vagus nerve was stimulated with a biphasic, charge-balanced pulse. Silk wire was strapped along the vagus nerve to cause reversible lesions of the nerve.
VNS induced an electrophysiological response consisting of a fast and a slow component. The threshold intensity was 2490{\textpm}240{\textmu}A and 2067{\textpm}247{\textmu}A respectively. The components reached their maximum amplitude at 3875{\textpm}530{\textmu}A and 3000{\textpm}935 {\textmu}A. Mean latency, at 2mm, was 0.4{\textpm}0.1ms and 2.6{\textpm}0.3ms. Conduction velocity for the fast component was 25m/s. The fast component disappeared by afferent lesioning the vagus nerve. The slow component disappeared by efferent lesioning, by lesioning the recurrent laryngeal nerve and by applying Vecuronium to the larynx muscles.
A short, single electrical pulse activates fast conducting afferent fibers. Also efferent fibers of the recurrent laryngeal nerve are activated resulting in contraction of larynx muscles. A far field potential was recorded on the vagus nerve. Our set-up can be used to evaluate the effect of stimulation parameters at the cervical vagus nerve in rat epilepsy models.},
  author       = {Mollet, Lies and Raedt, Robrecht and EL TAHRY, RIEM and Delbeke, Jean and De Herdt, Veerle and Meurs, Alfred and Wadman, Wytse and Vonck, Kristl and Boon, Paul},
  booktitle    = {EPILEPSIA},
  issn         = {0013-9580},
  language     = {eng},
  location     = {Rome, Italy},
  number       = {suppl. 6},
  pages        = {160--160},
  title        = {Electrophysiological responses to vagus nerve stimulation in rats},
  volume       = {52},
  year         = {2011},
}

Chicago
Mollet, Lies, Robrecht Raedt, RIEM EL TAHRY, Jean Delbeke, VEERLE DE HERDT, Alfred Meurs, Wytse Wadman, Kristl Vonck, and Paul Boon. 2011. “Electrophysiological Responses to Vagus Nerve Stimulation in Rats.” In Epilepsia, 52:160–160.
APA
Mollet, L., Raedt, R., EL TAHRY, R., Delbeke, J., DE HERDT, V., Meurs, A., Wadman, W., et al. (2011). Electrophysiological responses to vagus nerve stimulation in rats. EPILEPSIA (Vol. 52, pp. 160–160). Presented at the 29th International Epilepsy Congress.
Vancouver
1.
Mollet L, Raedt R, EL TAHRY R, Delbeke J, DE HERDT V, Meurs A, et al. Electrophysiological responses to vagus nerve stimulation in rats. EPILEPSIA. 2011. p. 160–160.
MLA
Mollet, Lies, Robrecht Raedt, RIEM EL TAHRY, et al. “Electrophysiological Responses to Vagus Nerve Stimulation in Rats.” Epilepsia. Vol. 52. 2011. 160–160. Print.