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Stereospecific effect of hexachlorocyclohexane on activity and structure of soil methanotrophic communities

Birgit Mertens, Nico Boon UGent and Willy Verstraete UGent (2005) ENVIRONMENTAL MICROBIOLOGY. 7(5). p.660-669
abstract
In the past decades, large amounts of non-insecticidal hexachlorocyclohexane (HCH) isomers (alpha-, beta-, delta- and epsilon-HCH) have been dumped as side-products of the insecticide gamma-HCH (lindane). This study investigates the effect of HCH isomers on methane oxidation, an important soil function performed by methanotrophic bacteria. Both activity and structure of the methanotrophic community were assessed, using methane oxidation assays and PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) respectively. Methane oxidation assays with historically polluted soils revealed that on the long-term methane oxidation was inhibited by HCH pollution. PCR-DGGE and diversity analysis based on Lorenz curves showed that the type I methanotrophic community was less evenly distributed in historically HCH-polluted soils compared with less polluted reference soils. Short-term experiments with methane-enriched consortia further demonstrated that only gamma- and delta-isomers inhibited methane oxidation. Type I methanotrophs of methane-enriched microbial consortia that received gamma- or delta-HCH evolved towards higher species richness. Apparently, for historically HCH-polluted soils, a narrow community remained after long-term exposure while in case of short-term exposures, methane-enriched consortia were converted into less active, but richer communities when they were stressed by the presence of gamma- or delta-HCH. This work demonstrates the importance of incorporating all isomers and possible other side-products in risk assessment studies of persistent organic pollutants and the use of structural analysis of type I methanotrophic communities as evaluating tool.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
GRADIENT GEL-ELECTROPHORESIS, 16S RIBOSOMAL-RNA, METHANE OXIDATION, PERSISTENT ORGANOCHLORINES, AMMONIA MONOOXYGENASE, ORGANIC POLLUTANTS, AGRICULTURAL SOIL, ARABLE SOIL, COVER SOIL, OXIC SOILS
journal title
ENVIRONMENTAL MICROBIOLOGY
Environ. Microbiol.
volume
7
issue
5
pages
660-669 pages
Web of Science type
Article
Web of Science id
000228266600006
JCR category
MICROBIOLOGY
JCR impact factor
4.559 (2005)
JCR rank
12/85 (2005)
JCR quartile
1 (2005)
ISSN
1462-2912
DOI
10.1111/j.1462-2920.2004.00735.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
305102
handle
http://hdl.handle.net/1854/LU-305102
date created
2005-05-10 15:12:00
date last changed
2016-12-19 15:45:35
@article{305102,
  abstract     = {In the past decades, large amounts of non-insecticidal hexachlorocyclohexane (HCH) isomers (alpha-, beta-, delta- and epsilon-HCH) have been dumped as side-products of the insecticide gamma-HCH (lindane). This study investigates the effect of HCH isomers on methane oxidation, an important soil function performed by methanotrophic bacteria. Both activity and structure of the methanotrophic community were assessed, using methane oxidation assays and PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) respectively. Methane oxidation assays with historically polluted soils revealed that on the long-term methane oxidation was inhibited by HCH pollution. PCR-DGGE and diversity analysis based on Lorenz curves showed that the type I methanotrophic community was less evenly distributed in historically HCH-polluted soils compared with less polluted reference soils. Short-term experiments with methane-enriched consortia further demonstrated that only gamma- and delta-isomers inhibited methane oxidation. Type I methanotrophs of methane-enriched microbial consortia that received gamma- or delta-HCH evolved towards higher species richness. Apparently, for historically HCH-polluted soils, a narrow community remained after long-term exposure while in case of short-term exposures, methane-enriched consortia were converted into less active, but richer communities when they were stressed by the presence of gamma- or delta-HCH. This work demonstrates the importance of incorporating all isomers and possible other side-products in risk assessment studies of persistent organic pollutants and the use of structural analysis of type I methanotrophic communities as evaluating tool.},
  author       = {Mertens, Birgit and Boon, Nico and Verstraete, Willy},
  issn         = {1462-2912},
  journal      = {ENVIRONMENTAL MICROBIOLOGY},
  keyword      = {GRADIENT GEL-ELECTROPHORESIS,16S RIBOSOMAL-RNA,METHANE OXIDATION,PERSISTENT ORGANOCHLORINES,AMMONIA MONOOXYGENASE,ORGANIC POLLUTANTS,AGRICULTURAL SOIL,ARABLE SOIL,COVER SOIL,OXIC SOILS},
  language     = {eng},
  number       = {5},
  pages        = {660--669},
  title        = {Stereospecific effect of hexachlorocyclohexane on activity and structure of soil methanotrophic communities},
  url          = {http://dx.doi.org/10.1111/j.1462-2920.2004.00735.x},
  volume       = {7},
  year         = {2005},
}

Chicago
Mertens, Birgit, Nico Boon, and Willy Verstraete. 2005. “Stereospecific Effect of Hexachlorocyclohexane on Activity and Structure of Soil Methanotrophic Communities.” Environmental Microbiology 7 (5): 660–669.
APA
Mertens, Birgit, Boon, N., & Verstraete, W. (2005). Stereospecific effect of hexachlorocyclohexane on activity and structure of soil methanotrophic communities. ENVIRONMENTAL MICROBIOLOGY, 7(5), 660–669.
Vancouver
1.
Mertens B, Boon N, Verstraete W. Stereospecific effect of hexachlorocyclohexane on activity and structure of soil methanotrophic communities. ENVIRONMENTAL MICROBIOLOGY. 2005;7(5):660–9.
MLA
Mertens, Birgit, Nico Boon, and Willy Verstraete. “Stereospecific Effect of Hexachlorocyclohexane on Activity and Structure of Soil Methanotrophic Communities.” ENVIRONMENTAL MICROBIOLOGY 7.5 (2005): 660–669. Print.