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Immunization with M2e-displaying T7 bacteriophage nanoparticles protects against Influenza A virus challenge

Hamidreza Hashemi, Somayeh Pouyanfard, Mojgan Bandehpour, Zahra Noroozbabaei, Bahram Kazemi, Xavier Saelens UGent and Talat Mokhtari-Azad (2012) PLOS ONE. 7(9).
abstract
Considering the emergence of highly pathogenic influenza viruses and threat of worldwide pandemics, there is an urgent need to develop broadly-protective influenza vaccines. In this study, we demonstrate the potential of T7 bacteriophage-based nanoparticles with genetically fused ectodomain of influenza A virus M2 protein (T7-M2e) as a candidate universal flu vaccine. Immunization of mice with non-adjuvanted T7-M2e elicited M2e-specific serum antibody responses that were similar in magnitude to those elicited by M2e peptide administered in Freund's adjuvant. Comparable IgG responses directed against T7 phage capsomers were induced following vaccination with wild type T7 or T7-M2e. T7-M2e immunization induced balanced amounts of IgG(1) and IgG(2a) antibodies and these antibodies specifically recognized native M2 on the surface of influenza A virus-infected mammalian cells. The frequency of IFN-gamma-secreting T cells induced by T7-M2e nanoparticles was comparable to those elicited by M2e peptide emulsified in Freund's adjuvant. Emulsification of T7-M2e nanoparticles in Freund's adjuvant, however, induced a significantly stronger T cell response. Furthermore, T7-M2e-immunized mice were protected against lethal challenge with an H1N1 or an H3N2 virus, implying the induction of heterosubtypic immunity in our mouse model. T7-M2e-immunized mice displayed considerable weight loss and had significantly reduced viral load in their lungs compared to controls. We conclude that display of M2e on the surface of T7 phage nanoparticles offers an efficient and economical opportunity to induce cross-protective M2e-based immunity against influenza A.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
M2 PROTEIN, MATRIX PROTEIN-2, MONOCLONAL-ANTIBODY, ANTIGENIC PEPTIDE VACCINE, PHAGE DISPLAY, EXTRACELLULAR DOMAIN, EFFICIENT INDUCTION, CAPSID SURFACE, BACTERIOPHAGE-T4, RESPONSES
journal title
PLOS ONE
PLoS One
volume
7
issue
9
article_number
e45765
pages
11 pages
Web of Science type
Article
Web of Science id
000309554700068
JCR category
MULTIDISCIPLINARY SCIENCES
JCR impact factor
3.73 (2012)
JCR rank
7/56 (2012)
JCR quartile
1 (2012)
ISSN
1932-6203
DOI
10.1371/journal.pone.0045765
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
3049348
handle
http://hdl.handle.net/1854/LU-3049348
date created
2012-11-08 13:17:41
date last changed
2012-11-09 09:26:42
@article{3049348,
  abstract     = {Considering the emergence of highly pathogenic influenza viruses and threat of worldwide pandemics, there is an urgent need to develop broadly-protective influenza vaccines. In this study, we demonstrate the potential of T7 bacteriophage-based nanoparticles with genetically fused ectodomain of influenza A virus M2 protein (T7-M2e) as a candidate universal flu vaccine. Immunization of mice with non-adjuvanted T7-M2e elicited M2e-specific serum antibody responses that were similar in magnitude to those elicited by M2e peptide administered in Freund's adjuvant. Comparable IgG responses directed against T7 phage capsomers were induced following vaccination with wild type T7 or T7-M2e. T7-M2e immunization induced balanced amounts of IgG(1) and IgG(2a) antibodies and these antibodies specifically recognized native M2 on the surface of influenza A virus-infected mammalian cells. The frequency of IFN-gamma-secreting T cells induced by T7-M2e nanoparticles was comparable to those elicited by M2e peptide emulsified in Freund's adjuvant. Emulsification of T7-M2e nanoparticles in Freund's adjuvant, however, induced a significantly stronger T cell response. Furthermore, T7-M2e-immunized mice were protected against lethal challenge with an H1N1 or an H3N2 virus, implying the induction of heterosubtypic immunity in our mouse model. T7-M2e-immunized mice displayed considerable weight loss and had significantly reduced viral load in their lungs compared to controls. We conclude that display of M2e on the surface of T7 phage nanoparticles offers an efficient and economical opportunity to induce cross-protective M2e-based immunity against influenza A.},
  articleno    = {e45765},
  author       = {Hashemi, Hamidreza and Pouyanfard, Somayeh and Bandehpour, Mojgan and Noroozbabaei, Zahra and Kazemi, Bahram and Saelens, Xavier and Mokhtari-Azad, Talat},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keyword      = {M2 PROTEIN,MATRIX PROTEIN-2,MONOCLONAL-ANTIBODY,ANTIGENIC PEPTIDE VACCINE,PHAGE DISPLAY,EXTRACELLULAR DOMAIN,EFFICIENT INDUCTION,CAPSID SURFACE,BACTERIOPHAGE-T4,RESPONSES},
  language     = {eng},
  number       = {9},
  pages        = {11},
  title        = {Immunization with M2e-displaying T7 bacteriophage nanoparticles protects against Influenza A virus challenge},
  url          = {http://dx.doi.org/10.1371/journal.pone.0045765},
  volume       = {7},
  year         = {2012},
}

Chicago
Hashemi, Hamidreza, Somayeh Pouyanfard, Mojgan Bandehpour, Zahra Noroozbabaei, Bahram Kazemi, Xavier Saelens, and Talat Mokhtari-Azad. 2012. “Immunization with M2e-displaying T7 Bacteriophage Nanoparticles Protects Against Influenza A Virus Challenge.” Plos One 7 (9).
APA
Hashemi, H., Pouyanfard, S., Bandehpour, M., Noroozbabaei, Z., Kazemi, B., Saelens, X., & Mokhtari-Azad, T. (2012). Immunization with M2e-displaying T7 bacteriophage nanoparticles protects against Influenza A virus challenge. PLOS ONE, 7(9).
Vancouver
1.
Hashemi H, Pouyanfard S, Bandehpour M, Noroozbabaei Z, Kazemi B, Saelens X, et al. Immunization with M2e-displaying T7 bacteriophage nanoparticles protects against Influenza A virus challenge. PLOS ONE. 2012;7(9).
MLA
Hashemi, Hamidreza, Somayeh Pouyanfard, Mojgan Bandehpour, et al. “Immunization with M2e-displaying T7 Bacteriophage Nanoparticles Protects Against Influenza A Virus Challenge.” PLOS ONE 7.9 (2012): n. pag. Print.