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Strain-specific transfer of antibiotic resistance from an environmental plasmid to foodborne pathogens

Eva Van Meervenne UGent, Els Van Coillie, Frederiek-Maarten Kerckhof UGent, Frank Devlieghere UGent, Lieve Herman, Leen De Gelder UGent, Eva M Top and Nico Boon UGent (2012) JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY.
abstract
Pathogens resistant to multiple antibiotics are rapidly emerging, entailing important consequences for human health. This study investigated if the broad-host-range multiresistance plasmid pB10, isolated from a wastewater treatment plant, harbouring amoxicillin, streptomycin, sulfonamide, and tetracycline resistance genes, was transferable to the foodborne pathogens Salmonella spp. or E. coli O157:H7 and how this transfer alters the phenotype of the recipients. The transfer ratio was determined by both plating and flow cytometry. Antibiotic resistance profiles were determined for both recipients and transconjugants using the disk diffusion method. For 14 of the 15 recipient strains, transconjugants were detected. Based on plating, transfer ratios were between 6.8 x 10(-9) and 3.0 x 10(-2) while using flow cytometry, transfer ratios were between <1.0 x 10(-5) and 1.9 x 10(-2). With a few exceptions, the transconjugants showed phenotypically increased resistance, indicating that most of the transferred resistance genes were expressed. In summary, we showed that an environmental plasmid can be transferred into foodborne pathogenic bacteria at high transfer ratios. However, the transfer ratio seemed to be recipient strain dependent. Moreover, the newly acquired resistance genes could turn antibiotic susceptible strains into resistant ones, paving the way to compromise human health.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
HORIZONTAL GENE-TRANSFER, ESCHERICHIA-COLI, ANTIMICROBIAL RESISTANCE, CONJUGATIVE TRANSFER, TETRACYCLINE RESISTANCE, POSITIVE BACTERIA, ACTIVATED-SLUDGE, VIRULENCE GENES, HOST-RANGE, FLOW-CYTOMETRY
journal title
JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY
J. Biomed. Biotechnol.
article_number
834598
pages
8 pages
Web of Science type
Article
Web of Science id
000307581100001
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
2.88 (2012)
JCR rank
50/157 (2012)
JCR quartile
2 (2012)
ISSN
1110-7243
DOI
10.1155/2012/834598
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
3047106
handle
http://hdl.handle.net/1854/LU-3047106
date created
2012-11-05 11:42:27
date last changed
2015-06-17 10:17:56
@article{3047106,
  abstract     = {Pathogens resistant to multiple antibiotics are rapidly emerging, entailing important consequences for human health. This study investigated if the broad-host-range multiresistance plasmid pB10, isolated from a wastewater treatment plant, harbouring amoxicillin, streptomycin, sulfonamide, and tetracycline resistance genes, was transferable to the foodborne pathogens Salmonella spp. or E. coli O157:H7 and how this transfer alters the phenotype of the recipients. The transfer ratio was determined by both plating and flow cytometry. Antibiotic resistance profiles were determined for both recipients and transconjugants using the disk diffusion method. For 14 of the 15 recipient strains, transconjugants were detected. Based on plating, transfer ratios were between 6.8 x 10(-9) and 3.0 x 10(-2) while using flow cytometry, transfer ratios were between {\textlangle}1.0 x 10(-5) and 1.9 x 10(-2). With a few exceptions, the transconjugants showed phenotypically increased resistance, indicating that most of the transferred resistance genes were expressed. In summary, we showed that an environmental plasmid can be transferred into foodborne pathogenic bacteria at high transfer ratios. However, the transfer ratio seemed to be recipient strain dependent. Moreover, the newly acquired resistance genes could turn antibiotic susceptible strains into resistant ones, paving the way to compromise human health.},
  articleno    = {834598},
  author       = {Van Meervenne, Eva and Van Coillie, Els and Kerckhof, Frederiek-Maarten and Devlieghere, Frank and Herman, Lieve and De Gelder, Leen and Top, Eva M and Boon, Nico},
  issn         = {1110-7243},
  journal      = {JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY},
  keyword      = {HORIZONTAL GENE-TRANSFER,ESCHERICHIA-COLI,ANTIMICROBIAL RESISTANCE,CONJUGATIVE TRANSFER,TETRACYCLINE RESISTANCE,POSITIVE BACTERIA,ACTIVATED-SLUDGE,VIRULENCE GENES,HOST-RANGE,FLOW-CYTOMETRY},
  language     = {eng},
  pages        = {8},
  title        = {Strain-specific transfer of antibiotic resistance from an environmental plasmid to foodborne pathogens},
  url          = {http://dx.doi.org/10.1155/2012/834598},
  year         = {2012},
}

Chicago
Van Meervenne, Eva, Els Van Coillie, Frederiek-Maarten Kerckhof, Frank Devlieghere, Lieve Herman, Leen De Gelder, Eva M Top, and Nico Boon. 2012. “Strain-specific Transfer of Antibiotic Resistance from an Environmental Plasmid to Foodborne Pathogens.” Journal of Biomedicine and Biotechnology.
APA
Van Meervenne, E., Van Coillie, E., Kerckhof, F.-M., Devlieghere, F., Herman, L., De Gelder, L., Top, E. M., et al. (2012). Strain-specific transfer of antibiotic resistance from an environmental plasmid to foodborne pathogens. JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY.
Vancouver
1.
Van Meervenne E, Van Coillie E, Kerckhof F-M, Devlieghere F, Herman L, De Gelder L, et al. Strain-specific transfer of antibiotic resistance from an environmental plasmid to foodborne pathogens. JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY. 2012;
MLA
Van Meervenne, Eva, Els Van Coillie, Frederiek-Maarten Kerckhof, et al. “Strain-specific Transfer of Antibiotic Resistance from an Environmental Plasmid to Foodborne Pathogens.” JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY (2012): n. pag. Print.