Ghent University Academic Bibliography

Advanced

IL-15 augments TCR-induced CD4⁺ T cell expansion in vitro by inhibiting the suppressive function of CD25High CD4⁺ T cells

Tom Van Belle, Hans Dooms, Tom Boonefaes, Xiao-Qing Wei, Georges Leclercq UGent and Johan Grooten UGent (2012) PLOS ONE. 7(9).
abstract
Due to its critical role in NK cell differentiation and CD8(+) T cell homeostasis, the importance of IL-15 is more firmly established for cytolytic effectors of the immune system than for CD4(+) T cells. The increased levels of IL-15 found in several CD4(+) T cell-driven (auto-) immune diseases prompted us to examine how IL-15 influences murine CD4(+) T cell responses to low dose TCR-stimulation in vitro. We show that IL-15 exerts growth factor activity on both CD4(+) and CD8+ T cells in a TCR-dependent and Cyclosporin A-sensitive manner. In CD4(+) T cells, IL-15 augmented initial IL-2-dependent expansion and once IL-15R alpha was upregulated, IL-15 sustained the TCR-induced expression of IL-2/15R beta, supporting proliferation independently of secreted IL-2. Moreover, IL-15 counteracts CD4(+) T cell suppression by a gradually expanding CD25(High)CD4(+) T cell subset that expresses Foxp3 and originates from CD4(+)CD25(+) Tregs. These in vitro data suggest that IL-15 may dramatically strengthen the T cell response to suboptimal TCR-triggering by overcoming an activation threshold set by Treg that might create a risk for autoimmune pathology.
Please use this url to cite or link to this publication:
author
organization
alternative title
IL-15 augments TCR-induced CD4+ T cell expansion in vitro by inhibiting the suppressive function of CD25High CD4+ T cells
IL-15 augments TCR-induced CD4(+) T cell expansion in vitro by inhibiting the suppressive function of CD25(High) CD4(+) T cells
year
type
journalArticle (original)
publication status
published
subject
keyword
RHEUMATOID-ARTHRITIS, INTERLEUKIN 15, IMMUNE-RESPONSES, DENDRITIC CELLS, RECEPTOR-ALPHA, SELECTIVE STIMULATION, MEDIATED SUPPRESSION, LYMPHOID HOMEOSTASIS, CYTOKINE PRODUCTION, IMMUNOLOGICAL SELF-TOLERANCE
journal title
PLOS ONE
PLoS One
volume
7
issue
9
article_number
e45299
pages
11 pages
Web of Science type
Article
Web of Science id
000309388900037
JCR category
MULTIDISCIPLINARY SCIENCES
JCR impact factor
3.73 (2012)
JCR rank
7/56 (2012)
JCR quartile
1 (2012)
ISSN
1932-6203
DOI
10.1371/journal.pone.0045299
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
3041811
handle
http://hdl.handle.net/1854/LU-3041811
date created
2012-11-05 09:28:16
date last changed
2012-11-05 13:58:22
@article{3041811,
  abstract     = {Due to its critical role in NK cell differentiation and CD8(+) T cell homeostasis, the importance of IL-15 is more firmly established for cytolytic effectors of the immune system than for CD4(+) T cells. The increased levels of IL-15 found in several CD4(+) T cell-driven (auto-) immune diseases prompted us to examine how IL-15 influences murine CD4(+) T cell responses to low dose TCR-stimulation in vitro. We show that IL-15 exerts growth factor activity on both CD4(+) and CD8+ T cells in a TCR-dependent and Cyclosporin A-sensitive manner. In CD4(+) T cells, IL-15 augmented initial IL-2-dependent expansion and once IL-15R alpha was upregulated, IL-15 sustained the TCR-induced expression of IL-2/15R beta, supporting proliferation independently of secreted IL-2. Moreover, IL-15 counteracts CD4(+) T cell suppression by a gradually expanding CD25(High)CD4(+) T cell subset that expresses Foxp3 and originates from CD4(+)CD25(+) Tregs. These in vitro data suggest that IL-15 may dramatically strengthen the T cell response to suboptimal TCR-triggering by overcoming an activation threshold set by Treg that might create a risk for autoimmune pathology.},
  articleno    = {e45299},
  author       = {Van Belle, Tom and Dooms, Hans and Boonefaes, Tom and Wei, Xiao-Qing and Leclercq, Georges and Grooten, Johan},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keyword      = {RHEUMATOID-ARTHRITIS,INTERLEUKIN 15,IMMUNE-RESPONSES,DENDRITIC CELLS,RECEPTOR-ALPHA,SELECTIVE STIMULATION,MEDIATED SUPPRESSION,LYMPHOID HOMEOSTASIS,CYTOKINE PRODUCTION,IMMUNOLOGICAL SELF-TOLERANCE},
  language     = {eng},
  number       = {9},
  pages        = {11},
  title        = {IL-15 augments TCR-induced CD4\unmatched{207a} T cell expansion in vitro by inhibiting the suppressive function of CD25High CD4\unmatched{207a} T cells},
  url          = {http://dx.doi.org/10.1371/journal.pone.0045299},
  volume       = {7},
  year         = {2012},
}

Chicago
Van Belle, Tom, Hans Dooms, Tom Boonefaes, Xiao-Qing Wei, Georges Leclercq, and Johan Grooten. 2012. “IL-15 Augments TCR-induced CD4+ T Cell Expansion in Vitro by Inhibiting the Suppressive Function of CD25High CD4+ T Cells.” Plos One 7 (9).
APA
Van Belle, T., Dooms, H., Boonefaes, T., Wei, X.-Q., Leclercq, G., & Grooten, J. (2012). IL-15 augments TCR-induced CD4+ T cell expansion in vitro by inhibiting the suppressive function of CD25High CD4+ T cells. PLOS ONE, 7(9).
Vancouver
1.
Van Belle T, Dooms H, Boonefaes T, Wei X-Q, Leclercq G, Grooten J. IL-15 augments TCR-induced CD4+ T cell expansion in vitro by inhibiting the suppressive function of CD25High CD4+ T cells. PLOS ONE. 2012;7(9).
MLA
Van Belle, Tom, Hans Dooms, Tom Boonefaes, et al. “IL-15 Augments TCR-induced CD4+ T Cell Expansion in Vitro by Inhibiting the Suppressive Function of CD25High CD4+ T Cells.” PLOS ONE 7.9 (2012): n. pag. Print.