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Ocular anterior segment dysgenesis upon ablation of p120 catenin in neural crest cells

Huiyu Tian UGent, Ellen Sanders UGent, Albert Reynolds, Frans Van Roy UGent and Jolanda van Hengel UGent (2012) INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE. 53(9). p.5139-5153
abstract
PURPOSE. Development of the ocular anterior segment depends largely on periocular mesenchyme cells, which are derived predominantly from neural crest cells (NCC). Specific and differential cell adhesion is expected to be instrumental in induction, migration, and differentiation of NCC. As p120 catenin (ctn) is an important component of cadherin-catenin cell adhesion complexes, we assessed its role in development of the anterior segment structure. METHODS. We generated conditional p120ctn(fl/fl); Wnt1Cre knockout mice and studied the effect of this gene ablation on eye development in vivo. In addition, p120ctn was knocked down in vitro. RESULTS. Wnt1Cre-mediated deletion of floxed p120ctn alleles in NCC resulted in serious ocular anterior segment dysgenesis (ASD), including iridocorneal angle closure, complete anterior chamber obliteration, iris and ciliary body hypoplasia, corneal malformation and opacity, and glaucoma-like defects. A completely penetrant phenotype was visible approximately three weeks after birth, but histologic defects were obvious at embryonal day 18.5 (E18.5). Neither migration of NCC nor expression of key transcription factors appeared to be affected. In contrast, the N-cadherin expression pattern was changed significantly in iridocorneal angle cells and corneal endothelium. A human trabecular meshwork cell line in which p120ctn was knocked down also showed decreased expression levels of N-cadherin and beta-catenin at the plasma membrane, but no defect in cell migration. CONCLUSIONS. p120ctn has a critical role in ocular mesenchyme development. Loss of p120ctn and the associated N-cadherin downregulation in NCC leads to ASD without affecting cell migration. p120ctn abnormalities might have a role in the pathophysiology of mammalian eye development.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
P120-CATENIN, ADHESION, VE-CADHERIN, N-CADHERIN, E-CADHERIN, DIFFERENTIATION, CANCER, EYE, EXPRESSION, ROLES
journal title
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
Invest. Ophthalmol. Vis. Sci.
volume
53
issue
9
pages
5139 - 5153
Web of Science type
Article
Web of Science id
000308695400008
JCR category
OPHTHALMOLOGY
JCR impact factor
3.441 (2012)
JCR rank
5/58 (2012)
JCR quartile
1 (2012)
ISSN
0146-0404
DOI
10.1167/iovs.12-9472
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
3040493
handle
http://hdl.handle.net/1854/LU-3040493
date created
2012-11-05 09:27:07
date last changed
2012-11-05 13:42:42
@article{3040493,
  abstract     = {PURPOSE. Development of the ocular anterior segment depends largely on periocular mesenchyme cells, which are derived predominantly from neural crest cells (NCC). Specific and differential cell adhesion is expected to be instrumental in induction, migration, and differentiation of NCC. As p120 catenin (ctn) is an important component of cadherin-catenin cell adhesion complexes, we assessed its role in development of the anterior segment structure.
METHODS. We generated conditional p120ctn(fl/fl); Wnt1Cre knockout mice and studied the effect of this gene ablation on eye development in vivo. In addition, p120ctn was knocked down in vitro.
RESULTS. Wnt1Cre-mediated deletion of floxed p120ctn alleles in NCC resulted in serious ocular anterior segment dysgenesis (ASD), including iridocorneal angle closure, complete anterior chamber obliteration, iris and ciliary body hypoplasia, corneal malformation and opacity, and glaucoma-like defects. A completely penetrant phenotype was visible approximately three weeks after birth, but histologic defects were obvious at embryonal day 18.5 (E18.5). Neither migration of NCC nor expression of key transcription factors appeared to be affected. In contrast, the N-cadherin expression pattern was changed significantly in iridocorneal angle cells and corneal endothelium. A human trabecular meshwork cell line in which p120ctn was knocked down also showed decreased expression levels of N-cadherin and beta-catenin at the plasma membrane, but no defect in cell migration.
CONCLUSIONS. p120ctn has a critical role in ocular mesenchyme development. Loss of p120ctn and the associated N-cadherin downregulation in NCC leads to ASD without affecting cell migration. p120ctn abnormalities might have a role in the pathophysiology of mammalian eye development.},
  author       = {Tian, Huiyu and Sanders, Ellen and Reynolds, Albert and Van Roy, Frans and van Hengel, Jolanda},
  issn         = {0146-0404},
  journal      = {INVESTIGATIVE OPHTHALMOLOGY \& VISUAL SCIENCE},
  keyword      = {P120-CATENIN,ADHESION,VE-CADHERIN,N-CADHERIN,E-CADHERIN,DIFFERENTIATION,CANCER,EYE,EXPRESSION,ROLES},
  language     = {eng},
  number       = {9},
  pages        = {5139--5153},
  title        = {Ocular anterior segment dysgenesis upon ablation of p120 catenin in neural crest cells},
  url          = {http://dx.doi.org/10.1167/iovs.12-9472},
  volume       = {53},
  year         = {2012},
}

Chicago
Tian, Huiyu, Ellen Sanders, Albert Reynolds, Frans Van Roy, and Jolanda van Hengel. 2012. “Ocular Anterior Segment Dysgenesis Upon Ablation of P120 Catenin in Neural Crest Cells.” Investigative Ophthalmology & Visual Science 53 (9): 5139–5153.
APA
Tian, H., Sanders, E., Reynolds, A., Van Roy, F., & van Hengel, J. (2012). Ocular anterior segment dysgenesis upon ablation of p120 catenin in neural crest cells. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 53(9), 5139–5153.
Vancouver
1.
Tian H, Sanders E, Reynolds A, Van Roy F, van Hengel J. Ocular anterior segment dysgenesis upon ablation of p120 catenin in neural crest cells. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE. 2012;53(9):5139–53.
MLA
Tian, Huiyu, Ellen Sanders, Albert Reynolds, et al. “Ocular Anterior Segment Dysgenesis Upon Ablation of P120 Catenin in Neural Crest Cells.” INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE 53.9 (2012): 5139–5153. Print.