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Effect of culture conditions on the achievable taxonomic resolution of Raman spectroscopy disclosed by three Bacillus species

Didier Hutsebaut, K Maquelin, Paul De Vos UGent, Peter Vandenabeele UGent, Luc Moens UGent and GJ Puppels (2004) ANALYTICAL CHEMISTRY. 76(21). p.6274-6281
abstract
Confocal micro-Raman spectroscopy requires a minimum of sample handling and no reagents and allows fast identification of microorganisms. Since it reflects the overall molecular composition of the cells, it provides much more information than classical, microbial analyses. However, since the molecular makeup of a cell depends on culture conditions, it can be argued that this will affect the reproducibility and discrimination ability of Raman spectroscopy. We used Bacillus cereus, Bacillus pumilus, and Bacillus licheniformis, which are known to be clearly distinct from each other and each displaying important phenotypic heterogeneity, in a wide variety of culture conditions to analyze this. It is illustrated that the influence of culture conditions on the identification accuracy and taxonomic resolution of Raman spectroscopy is important though the effect on the final identification is limited within the set of stains studied. Furthermore, some conditions even allow for better discrimination than others. From a practical point of view, it is especially important that differences in culturing time (and culturing temperature) can be accommodated.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
TRANSFORM-INFRARED-SPECTROSCOPY, FT-IR SPECTROSCOPY, RAPID IDENTIFICATION, VIBRATIONAL SPECTROSCOPY, RELEVANT MICROORGANISMS, BACTERIAL-CELLS, GENUS BACILLUS, MICROSPECTROSCOPY, CLASSIFICATION, PERFORMANCE
journal title
ANALYTICAL CHEMISTRY
Anal. Chem.
volume
76
issue
21
pages
6274 - 6281
Web of Science type
Article
Web of Science id
000224841300021
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
5.45 (2004)
JCR rank
2/70 (2004)
JCR quartile
1 (2004)
ISSN
0003-2700
DOI
10.1021/ac049228l
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
303973
handle
http://hdl.handle.net/1854/LU-303973
date created
2005-05-09 10:34:00
date last changed
2018-01-29 12:18:32
@article{303973,
  abstract     = {Confocal micro-Raman spectroscopy requires a minimum of sample handling and no reagents and allows fast identification of microorganisms. Since it reflects the overall molecular composition of the cells, it provides much more information than classical, microbial analyses. However, since the molecular makeup of a cell depends on culture conditions, it can be argued that this will affect the reproducibility and discrimination ability of Raman spectroscopy. We used Bacillus cereus, Bacillus pumilus, and Bacillus licheniformis, which are known to be clearly distinct from each other and each displaying important phenotypic heterogeneity, in a wide variety of culture conditions to analyze this. It is illustrated that the influence of culture conditions on the identification accuracy and taxonomic resolution of Raman spectroscopy is important though the effect on the final identification is limited within the set of stains studied. Furthermore, some conditions even allow for better discrimination than others. From a practical point of view, it is especially important that differences in culturing time (and culturing temperature) can be accommodated.},
  author       = {Hutsebaut, Didier and Maquelin, K and De Vos, Paul and Vandenabeele, Peter and Moens, Luc and Puppels, GJ},
  issn         = {0003-2700},
  journal      = {ANALYTICAL CHEMISTRY},
  keyword      = {TRANSFORM-INFRARED-SPECTROSCOPY,FT-IR SPECTROSCOPY,RAPID IDENTIFICATION,VIBRATIONAL SPECTROSCOPY,RELEVANT MICROORGANISMS,BACTERIAL-CELLS,GENUS BACILLUS,MICROSPECTROSCOPY,CLASSIFICATION,PERFORMANCE},
  language     = {eng},
  number       = {21},
  pages        = {6274--6281},
  title        = {Effect of culture conditions on the achievable taxonomic resolution of Raman spectroscopy disclosed by three Bacillus species},
  url          = {http://dx.doi.org/10.1021/ac049228l},
  volume       = {76},
  year         = {2004},
}

Chicago
Hutsebaut, Didier, K Maquelin, Paul De Vos, Peter Vandenabeele, Luc Moens, and GJ Puppels. 2004. “Effect of Culture Conditions on the Achievable Taxonomic Resolution of Raman Spectroscopy Disclosed by Three Bacillus Species.” Analytical Chemistry 76 (21): 6274–6281.
APA
Hutsebaut, Didier, Maquelin, K., De Vos, P., Vandenabeele, P., Moens, L., & Puppels, G. (2004). Effect of culture conditions on the achievable taxonomic resolution of Raman spectroscopy disclosed by three Bacillus species. ANALYTICAL CHEMISTRY, 76(21), 6274–6281.
Vancouver
1.
Hutsebaut D, Maquelin K, De Vos P, Vandenabeele P, Moens L, Puppels G. Effect of culture conditions on the achievable taxonomic resolution of Raman spectroscopy disclosed by three Bacillus species. ANALYTICAL CHEMISTRY. 2004;76(21):6274–81.
MLA
Hutsebaut, Didier, K Maquelin, Paul De Vos, et al. “Effect of Culture Conditions on the Achievable Taxonomic Resolution of Raman Spectroscopy Disclosed by Three Bacillus Species.” ANALYTICAL CHEMISTRY 76.21 (2004): 6274–6281. Print.