Overexpression of the oil palm (Elaeis guineensis Jacq.) TAPETUM DEVELOPMENT1-like Eg707 in rice affects cell division and differentiation and reduces fertility
- Author
- Le Van Thuc, Danny Geelen (UGent) , Huynh Ky, Siew-Eng Ooi, Suhaimi B Napis, Uma Rani Sinniah and Parameswari Namasivayam
- Organization
- Abstract
- The functional analysis of the TAPETUM DEVELOPMENT1-like analog Eg707 of oil palm was carried out in rice by over-expressing Eg707 under the control of a double cauliflower mosaic virus 35S promoter. Ectopic expression of Eg707 in rice induced dark green and matured compact brownish calli compared to pale wild type and negative control calli. Regenerated transgenic rice plants exhibited a reduction in organ size and plant height, rolled, erect leaves, less tillers, increased chlorophyll content, and reduced fertility with smaller green seeds. At the molecular level Eg707 overexpression caused an increase in the transcription of SAPK9, a SnRK2 protein kinase family member that is activated by ABA and hyperosmotic stress. Together, the results show that ectopic Eg707 expression influences cell division and differentiation, presumably via altered hormone homeostasis.
- Keywords
- Oil palm, Hormone signaling, Abscisic acid, Tissue culture, ENDOGENOUS REFERENCE GENE, EXPRESSED SEQUENCE TAGS, ABSCISIC ACID-DEFICIENT, ESCULENTUM MILL SEEDS, TIME QUANTITATIVE PCR, T-DNA, AGROBACTERIUM-TUMEFACIENS, GIBBERELLIN-DEFICIENT, FUNCTIONAL GENOMICS, TOBACCO PLANTS
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-3037034
- MLA
- Thuc, Le Van, et al. “Overexpression of the Oil Palm (Elaeis Guineensis Jacq.) TAPETUM DEVELOPMENT1-like Eg707 in Rice Affects Cell Division and Differentiation and Reduces Fertility.” MOLECULAR BIOLOGY REPORTS, vol. 40, no. 2, 2013, pp. 1579–90, doi:10.1007/s11033-012-2206-7.
- APA
- Thuc, L. V., Geelen, D., Ky, H., Ooi, S.-E., Napis, S. B., Sinniah, U. R., & Namasivayam, P. (2013). Overexpression of the oil palm (Elaeis guineensis Jacq.) TAPETUM DEVELOPMENT1-like Eg707 in rice affects cell division and differentiation and reduces fertility. MOLECULAR BIOLOGY REPORTS, 40(2), 1579–1590. https://doi.org/10.1007/s11033-012-2206-7
- Chicago author-date
- Thuc, Le Van, Danny Geelen, Huynh Ky, Siew-Eng Ooi, Suhaimi B Napis, Uma Rani Sinniah, and Parameswari Namasivayam. 2013. “Overexpression of the Oil Palm (Elaeis Guineensis Jacq.) TAPETUM DEVELOPMENT1-like Eg707 in Rice Affects Cell Division and Differentiation and Reduces Fertility.” MOLECULAR BIOLOGY REPORTS 40 (2): 1579–90. https://doi.org/10.1007/s11033-012-2206-7.
- Chicago author-date (all authors)
- Thuc, Le Van, Danny Geelen, Huynh Ky, Siew-Eng Ooi, Suhaimi B Napis, Uma Rani Sinniah, and Parameswari Namasivayam. 2013. “Overexpression of the Oil Palm (Elaeis Guineensis Jacq.) TAPETUM DEVELOPMENT1-like Eg707 in Rice Affects Cell Division and Differentiation and Reduces Fertility.” MOLECULAR BIOLOGY REPORTS 40 (2): 1579–1590. doi:10.1007/s11033-012-2206-7.
- Vancouver
- 1.Thuc LV, Geelen D, Ky H, Ooi S-E, Napis SB, Sinniah UR, et al. Overexpression of the oil palm (Elaeis guineensis Jacq.) TAPETUM DEVELOPMENT1-like Eg707 in rice affects cell division and differentiation and reduces fertility. MOLECULAR BIOLOGY REPORTS. 2013;40(2):1579–90.
- IEEE
- [1]L. V. Thuc et al., “Overexpression of the oil palm (Elaeis guineensis Jacq.) TAPETUM DEVELOPMENT1-like Eg707 in rice affects cell division and differentiation and reduces fertility,” MOLECULAR BIOLOGY REPORTS, vol. 40, no. 2, pp. 1579–1590, 2013.
@article{3037034, abstract = {{The functional analysis of the TAPETUM DEVELOPMENT1-like analog Eg707 of oil palm was carried out in rice by over-expressing Eg707 under the control of a double cauliflower mosaic virus 35S promoter. Ectopic expression of Eg707 in rice induced dark green and matured compact brownish calli compared to pale wild type and negative control calli. Regenerated transgenic rice plants exhibited a reduction in organ size and plant height, rolled, erect leaves, less tillers, increased chlorophyll content, and reduced fertility with smaller green seeds. At the molecular level Eg707 overexpression caused an increase in the transcription of SAPK9, a SnRK2 protein kinase family member that is activated by ABA and hyperosmotic stress. Together, the results show that ectopic Eg707 expression influences cell division and differentiation, presumably via altered hormone homeostasis.}}, author = {{Thuc, Le Van and Geelen, Danny and Ky, Huynh and Ooi, Siew-Eng and Napis, Suhaimi B and Sinniah, Uma Rani and Namasivayam, Parameswari}}, issn = {{0301-4851}}, journal = {{MOLECULAR BIOLOGY REPORTS}}, keywords = {{Oil palm,Hormone signaling,Abscisic acid,Tissue culture,ENDOGENOUS REFERENCE GENE,EXPRESSED SEQUENCE TAGS,ABSCISIC ACID-DEFICIENT,ESCULENTUM MILL SEEDS,TIME QUANTITATIVE PCR,T-DNA,AGROBACTERIUM-TUMEFACIENS,GIBBERELLIN-DEFICIENT,FUNCTIONAL GENOMICS,TOBACCO PLANTS}}, language = {{eng}}, number = {{2}}, pages = {{1579--1590}}, title = {{Overexpression of the oil palm (Elaeis guineensis Jacq.) TAPETUM DEVELOPMENT1-like Eg707 in rice affects cell division and differentiation and reduces fertility}}, url = {{http://doi.org/10.1007/s11033-012-2206-7}}, volume = {{40}}, year = {{2013}}, }
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