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AUX/LAX genes encode a family of auxin influx transporters that perform distinct functions during Arabidopsis development

Benjamin Péret, Kamal Swarup, Alison Ferguson, Malvika Seth, Yaodong Yang, Stijn Dhondt UGent, Nicholas James, Ilda Casimiro, Paula Perry and Adnan Syed, et al. (2012) PLANT CELL. 24(7). p.2874-2885
abstract
Auxin transport, which is mediated by specialized influx and efflux carriers, plays a major role in many aspects of plant growth and development. AUXIN1 (AUX1) has been demonstrated to encode a high-affinity auxin influx carrier. In Arabidopsis thaliana, AUX1 belongs to a small multigene family comprising four highly conserved genes (i.e., AUX1 and LIKE AUX1 [LAX] genes LAX1, LAX2, and LAX3). We report that all four members of this AUX/LAX family display auxin uptake functions. Despite the conservation of their biochemical function, AUX1, LAX1, and LAX3 have been described to regulate distinct auxin-dependent developmental processes. Here, we report that LAX2 regulates vascular patterning in cotyledons. We also describe how regulatory and coding sequences of AUX/LAX genes have undergone subfunctionalization based on their distinct patterns of spatial expression and the inability of LAX sequences to rescue aux1 mutant phenotypes, respectively. Despite their high sequence similarity at the protein level, transgenic studies reveal that LAX proteins are not correctly targeted in the AUX1 expression domain. Domain swapping studies suggest that the N-terminal half of AUX1 is essential for correct LAX localization. We conclude that Arabidopsis AUX/LAX genes encode a family of auxin influx transporters that perform distinct developmental functions and have evolved distinct regulatory mechanisms.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PLANT DEVELOPMENT, ROOT GRAVITROPISM, PERMEASE AUX1, PIN PROTEINS, HAIR-CELLS, LOCALIZATION, EFFLUX, EXPRESSION, LAX3, PATHWAY
journal title
PLANT CELL
Plant Cell
volume
24
issue
7
pages
2874 - 2885
Web of Science type
Article
Web of Science id
000308352800015
JCR category
PLANT SCIENCES
JCR impact factor
9.251 (2012)
JCR rank
4/193 (2012)
JCR quartile
1 (2012)
ISSN
1040-4651
DOI
10.1105/tpc.112.097766
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
3035451
handle
http://hdl.handle.net/1854/LU-3035451
date created
2012-10-26 15:16:46
date last changed
2012-12-17 16:42:47
@article{3035451,
  abstract     = {Auxin transport, which is mediated by specialized influx and efflux carriers, plays a major role in many aspects of plant growth and development. AUXIN1 (AUX1) has been demonstrated to encode a high-affinity auxin influx carrier. In Arabidopsis thaliana, AUX1 belongs to a small multigene family comprising four highly conserved genes (i.e., AUX1 and LIKE AUX1 [LAX] genes LAX1, LAX2, and LAX3). We report that all four members of this AUX/LAX family display auxin uptake functions. Despite the conservation of their biochemical function, AUX1, LAX1, and LAX3 have been described to regulate distinct auxin-dependent developmental processes. Here, we report that LAX2 regulates vascular patterning in cotyledons. We also describe how regulatory and coding sequences of AUX/LAX genes have undergone subfunctionalization based on their distinct patterns of spatial expression and the inability of LAX sequences to rescue aux1 mutant phenotypes, respectively. Despite their high sequence similarity at the protein level, transgenic studies reveal that LAX proteins are not correctly targeted in the AUX1 expression domain. Domain swapping studies suggest that the N-terminal half of AUX1 is essential for correct LAX localization. We conclude that Arabidopsis AUX/LAX genes encode a family of auxin influx transporters that perform distinct developmental functions and have evolved distinct regulatory mechanisms.},
  author       = {P{\'e}ret, Benjamin and Swarup, Kamal and Ferguson, Alison and Seth, Malvika and Yang, Yaodong and Dhondt, Stijn and James, Nicholas and Casimiro, Ilda and Perry, Paula and Syed, Adnan and Yang, Haibing and Reemmer, Jesica and Venison, Edward and Howells, Caroline and Perez-Amador, Miguel A and Yun, Jeonga G and Alonso, Jose and Beemster, Gerrit TS and Laplaze, Laurent and Murphy, Angus and Bennett, Malcolm J and Nielsen, Erik and Swarup, Ranjan},
  issn         = {1040-4651},
  journal      = {PLANT CELL},
  keyword      = {PLANT DEVELOPMENT,ROOT GRAVITROPISM,PERMEASE AUX1,PIN PROTEINS,HAIR-CELLS,LOCALIZATION,EFFLUX,EXPRESSION,LAX3,PATHWAY},
  language     = {eng},
  number       = {7},
  pages        = {2874--2885},
  title        = {AUX/LAX genes encode a family of auxin influx transporters that perform distinct functions during Arabidopsis development},
  url          = {http://dx.doi.org/10.1105/tpc.112.097766},
  volume       = {24},
  year         = {2012},
}

Chicago
Péret, Benjamin, Kamal Swarup, Alison Ferguson, Malvika Seth, Yaodong Yang, Stijn Dhondt, Nicholas James, et al. 2012. “AUX/LAX Genes Encode a Family of Auxin Influx Transporters That Perform Distinct Functions During Arabidopsis Development.” Plant Cell 24 (7): 2874–2885.
APA
Péret, B., Swarup, K., Ferguson, A., Seth, M., Yang, Y., Dhondt, S., James, N., et al. (2012). AUX/LAX genes encode a family of auxin influx transporters that perform distinct functions during Arabidopsis development. PLANT CELL, 24(7), 2874–2885.
Vancouver
1.
Péret B, Swarup K, Ferguson A, Seth M, Yang Y, Dhondt S, et al. AUX/LAX genes encode a family of auxin influx transporters that perform distinct functions during Arabidopsis development. PLANT CELL. 2012;24(7):2874–85.
MLA
Péret, Benjamin, Kamal Swarup, Alison Ferguson, et al. “AUX/LAX Genes Encode a Family of Auxin Influx Transporters That Perform Distinct Functions During Arabidopsis Development.” PLANT CELL 24.7 (2012): 2874–2885. Print.