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BEX5/RabA1b regulates trans-Golgi network-to-plasma membrane protein trafficking in Arabidopsis

Elena Feraru UGent, Ioan Mugurel Feraru UGent, Rin Asaoka, Tomasz Paciorek, Riet De Rycke UGent, Hirokazu Tanaka UGent, Akihiko Nakano and Jiri Friml UGent (2012) PLANT CELL. 24(7). p.3074-3086
abstract
Constitutive endocytic recycling is a crucial mechanism allowing regulation of the activity of proteins at the plasma membrane and for rapid changes in their localization, as demonstrated in plants for PIN-FORMED (PIN) proteins, the auxin transporters. To identify novel molecular components of endocytic recycling, mainly exocytosis, we designed a PIN1-green fluorescent protein fluorescence imaging-based forward genetic screen for Arabidopsis thaliana mutants that showed increased intracellular accumulation of cargos in response to the trafficking inhibitor brefeldin A (BFA). We identified bex5 (for BFA-visualized exocytic trafficking defective), a novel dominant mutant carrying a missense mutation that disrupts a conserved sequence motif of the small GTPase, RAS GENES FROM RAT BRAINA1b. bex5 displays defects such as enhanced protein accumulation in abnormal BFA compartments, aberrant endosomes, and defective exocytosis and transcytosis. BEX5/RabA1b localizes to trans-Golgi network/early endosomes (TGN/EE) and acts on distinct trafficking processes like those regulated by GTP exchange factors on ADP-ribosylation factors GNOM-LIKE1 and HOPM INTERACTOR7/BFA-VISUALIZED ENDOCYTIC TRAFFICKING DEFECTIVE1, which regulate trafficking at the Golgi apparatus and TGN/EE, respectively. All together, this study identifies Arabidopsis BEX5/RabA1b as a novel regulator of protein trafficking from a TGN/EE compartment to the plasma membrane.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
EFFLUX, RAB, LOCALIZATION, GTPASES, HIGHER-PLANTS, ENDOCYTOSIS, CELLS, ENDOPLASMIC-RETICULUM, COATED VESICLES, DEPENDENT AUXIN GRADIENTS
journal title
PLANT CELL
Plant Cell
volume
24
issue
7
pages
3074 - 3086
Web of Science type
Article
Web of Science id
000308352800028
JCR category
PLANT SCIENCES
JCR impact factor
9.251 (2012)
JCR rank
4/193 (2012)
JCR quartile
1 (2012)
ISSN
1040-4651
DOI
10.1105/tpc.112.098152
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
3035374
handle
http://hdl.handle.net/1854/LU-3035374
date created
2012-10-26 14:54:32
date last changed
2012-12-17 16:41:33
@article{3035374,
  abstract     = {Constitutive endocytic recycling is a crucial mechanism allowing regulation of the activity of proteins at the plasma membrane and for rapid changes in their localization, as demonstrated in plants for PIN-FORMED (PIN) proteins, the auxin transporters. To identify novel molecular components of endocytic recycling, mainly exocytosis, we designed a PIN1-green fluorescent protein fluorescence imaging-based forward genetic screen for Arabidopsis thaliana mutants that showed increased intracellular accumulation of cargos in response to the trafficking inhibitor brefeldin A (BFA). We identified bex5 (for BFA-visualized exocytic trafficking defective), a novel dominant mutant carrying a missense mutation that disrupts a conserved sequence motif of the small GTPase, RAS GENES FROM RAT BRAINA1b. bex5 displays defects such as enhanced protein accumulation in abnormal BFA compartments, aberrant endosomes, and defective exocytosis and transcytosis. BEX5/RabA1b localizes to trans-Golgi network/early endosomes (TGN/EE) and acts on distinct trafficking processes like those regulated by GTP exchange factors on ADP-ribosylation factors GNOM-LIKE1 and HOPM INTERACTOR7/BFA-VISUALIZED ENDOCYTIC TRAFFICKING DEFECTIVE1, which regulate trafficking at the Golgi apparatus and TGN/EE, respectively. All together, this study identifies Arabidopsis BEX5/RabA1b as a novel regulator of protein trafficking from a TGN/EE compartment to the plasma membrane.},
  author       = {Feraru, Elena and Feraru, Ioan Mugurel and Asaoka, Rin and Paciorek, Tomasz and De Rycke, Riet and Tanaka, Hirokazu and Nakano, Akihiko and Friml, Jiri},
  issn         = {1040-4651},
  journal      = {PLANT CELL},
  keyword      = {EFFLUX,RAB,LOCALIZATION,GTPASES,HIGHER-PLANTS,ENDOCYTOSIS,CELLS,ENDOPLASMIC-RETICULUM,COATED VESICLES,DEPENDENT AUXIN GRADIENTS},
  language     = {eng},
  number       = {7},
  pages        = {3074--3086},
  title        = {BEX5/RabA1b regulates trans-Golgi network-to-plasma membrane protein trafficking in Arabidopsis},
  url          = {http://dx.doi.org/10.1105/tpc.112.098152},
  volume       = {24},
  year         = {2012},
}

Chicago
Feraru, Elena, Ioan Mugurel Feraru, Rin Asaoka, Tomasz Paciorek, Riet De Rycke, Hirokazu Tanaka, Akihiko Nakano, and Jiri Friml. 2012. “BEX5/RabA1b Regulates trans-Golgi Network-to-plasma Membrane Protein Trafficking in Arabidopsis.” Plant Cell 24 (7): 3074–3086.
APA
Feraru, E., Feraru, I. M., Asaoka, R., Paciorek, T., De Rycke, R., Tanaka, H., Nakano, A., et al. (2012). BEX5/RabA1b regulates trans-Golgi network-to-plasma membrane protein trafficking in Arabidopsis. PLANT CELL, 24(7), 3074–3086.
Vancouver
1.
Feraru E, Feraru IM, Asaoka R, Paciorek T, De Rycke R, Tanaka H, et al. BEX5/RabA1b regulates trans-Golgi network-to-plasma membrane protein trafficking in Arabidopsis. PLANT CELL. 2012;24(7):3074–86.
MLA
Feraru, Elena, Ioan Mugurel Feraru, Rin Asaoka, et al. “BEX5/RabA1b Regulates trans-Golgi Network-to-plasma Membrane Protein Trafficking in Arabidopsis.” PLANT CELL 24.7 (2012): 3074–3086. Print.