Evidence for a role of Arabidopsis CDT1 proteins in gametophyte development and maintenance of genome integrity
- Author
- Séverine Domenichini, Moussa Benhamed, Geert De Jaeger (UGent) , Eveline Van De Slijke (UGent) , Sophie Blanchet, Mickaël Bourge, Lieven De Veylder (UGent) , Catherine Bergounioux and Cécile Raynaud
- Organization
- Abstract
- Meristems retain the ability to divide throughout the life cycle of plants, which can last for over 1000 years in some species. Furthermore, the germline is not laid down early during embryogenesis but originates from the meristematic cells relatively late during development. Thus, accurate cell cycle regulation is of utmost importance to avoid the accumulation of mutations during vegetative growth and reproduction. The Arabidopsis thaliana genome encodes two homologs of the replication licensing factor CDC10 Target1 (CDT1), and overexpression of CDT1a stimulates DNA replication. Here, we have investigated the respective functions of Arabidopsis CDT1a and CDT1b. We show that CDT1 proteins have partially redundant functions during gametophyte development and are required for the maintenance of genome integrity. Furthermore, CDT1-RNAi plants show endogenous DNA stress, are more tolerant than the wild type to DNA-damaging agents, and show constitutive induction of genes involved in DNA repair. This DNA stress response may be a direct consequence of reduced CDT1 accumulation on DNA repair or may relate to the ability of CDT1 proteins to form complexes with DNA polymerase e, which functions in DNA replication and in DNA stress checkpoint activation. Taken together, our results provide evidence for a crucial role of Arabidopsis CDT1 proteins in genome stability.
- Keywords
- DNA-POLYMERASE-EPSILON, TANDEM AFFINITY PURIFICATION, CELL-CYCLE, PROLIFERATION, FEMALE GAMETOPHYTE, PLANT DEVELOPMENT, S-PHASE, COMPLEX, REPLICATION, THALIANA
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-3035353
- MLA
- Domenichini, Séverine, et al. “Evidence for a Role of Arabidopsis CDT1 Proteins in Gametophyte Development and Maintenance of Genome Integrity.” PLANT CELL, vol. 24, no. 7, 2012, pp. 2779–91, doi:10.1105/tpc.112.100156.
- APA
- Domenichini, S., Benhamed, M., De Jaeger, G., Van De Slijke, E., Blanchet, S., Bourge, M., … Raynaud, C. (2012). Evidence for a role of Arabidopsis CDT1 proteins in gametophyte development and maintenance of genome integrity. PLANT CELL, 24(7), 2779–2791. https://doi.org/10.1105/tpc.112.100156
- Chicago author-date
- Domenichini, Séverine, Moussa Benhamed, Geert De Jaeger, Eveline Van De Slijke, Sophie Blanchet, Mickaël Bourge, Lieven De Veylder, Catherine Bergounioux, and Cécile Raynaud. 2012. “Evidence for a Role of Arabidopsis CDT1 Proteins in Gametophyte Development and Maintenance of Genome Integrity.” PLANT CELL 24 (7): 2779–91. https://doi.org/10.1105/tpc.112.100156.
- Chicago author-date (all authors)
- Domenichini, Séverine, Moussa Benhamed, Geert De Jaeger, Eveline Van De Slijke, Sophie Blanchet, Mickaël Bourge, Lieven De Veylder, Catherine Bergounioux, and Cécile Raynaud. 2012. “Evidence for a Role of Arabidopsis CDT1 Proteins in Gametophyte Development and Maintenance of Genome Integrity.” PLANT CELL 24 (7): 2779–2791. doi:10.1105/tpc.112.100156.
- Vancouver
- 1.Domenichini S, Benhamed M, De Jaeger G, Van De Slijke E, Blanchet S, Bourge M, et al. Evidence for a role of Arabidopsis CDT1 proteins in gametophyte development and maintenance of genome integrity. PLANT CELL. 2012;24(7):2779–91.
- IEEE
- [1]S. Domenichini et al., “Evidence for a role of Arabidopsis CDT1 proteins in gametophyte development and maintenance of genome integrity,” PLANT CELL, vol. 24, no. 7, pp. 2779–2791, 2012.
@article{3035353,
abstract = {{Meristems retain the ability to divide throughout the life cycle of plants, which can last for over 1000 years in some species. Furthermore, the germline is not laid down early during embryogenesis but originates from the meristematic cells relatively late during development. Thus, accurate cell cycle regulation is of utmost importance to avoid the accumulation of mutations during vegetative growth and reproduction. The Arabidopsis thaliana genome encodes two homologs of the replication licensing factor CDC10 Target1 (CDT1), and overexpression of CDT1a stimulates DNA replication. Here, we have investigated the respective functions of Arabidopsis CDT1a and CDT1b. We show that CDT1 proteins have partially redundant functions during gametophyte development and are required for the maintenance of genome integrity. Furthermore, CDT1-RNAi plants show endogenous DNA stress, are more tolerant than the wild type to DNA-damaging agents, and show constitutive induction of genes involved in DNA repair. This DNA stress response may be a direct consequence of reduced CDT1 accumulation on DNA repair or may relate to the ability of CDT1 proteins to form complexes with DNA polymerase e, which functions in DNA replication and in DNA stress checkpoint activation. Taken together, our results provide evidence for a crucial role of Arabidopsis CDT1 proteins in genome stability.}},
author = {{Domenichini, Séverine and Benhamed, Moussa and De Jaeger, Geert and Van De Slijke, Eveline and Blanchet, Sophie and Bourge, Mickaël and De Veylder, Lieven and Bergounioux, Catherine and Raynaud, Cécile}},
issn = {{1040-4651}},
journal = {{PLANT CELL}},
keywords = {{DNA-POLYMERASE-EPSILON,TANDEM AFFINITY PURIFICATION,CELL-CYCLE,PROLIFERATION,FEMALE GAMETOPHYTE,PLANT DEVELOPMENT,S-PHASE,COMPLEX,REPLICATION,THALIANA}},
language = {{eng}},
number = {{7}},
pages = {{2779--2791}},
title = {{Evidence for a role of Arabidopsis CDT1 proteins in gametophyte development and maintenance of genome integrity}},
url = {{http://doi.org/10.1105/tpc.112.100156}},
volume = {{24}},
year = {{2012}},
}
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