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Transport of nanoparticles in cystic fibrosis sputum and bacterial biofilms by single-particle tracking microscopy

Katrien Forier (UGent), Anne-Sophie Messiaen, Koen Raemdonck (UGent), Hendrik Deschout (UGent), Joanna Rejman (UGent), Frans De Baets (UGent), Hans Nelis (UGent), Stefaan De Smedt (UGent), Jo Demeester (UGent), Tom Coenye (UGent), et al.
(2013) NANOMEDICINE. 8(6). p.935-949
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Center for nano- and biophotonics (NB-Photonics)
Project
Center for nano- and biophotonics (NB-Photonics)
Abstract
Aim: The aim of this study was to evaluate the effect of the surface functionalization of model nanoparticles on their mobility in bacterial biofilms and cystic fibrosis sputum. Materials & methods: With single-particle tracking microscopy, the mobility of 0.1- and 0.2-mu m fluorescent polyethylene glycol (PEG) modified, carboxylate- and N,N-dimethylethylenediamine-modified polystyrene nanospheres were evaluated in fresh cystic fibrosis sputum, as well as Burkholderia multivorans and Pseudomonas aeruginosa biofilms. Results: PEGylation increased the mobility of the particles in sputum and biofilms, while the charged nanospheres were strongly immobilized. However, the transport of the PEGylated nanoparticles was lower in sputum compared with biofilms. Furthermore, the particle transport showed heterogeneity in samples originating from different patients. Conclusion: This study's data suggest that for future nanocarrier design it will be essential to combine PEGylation with a targeting moiety to ensure sufficient mobility in mucus and a better accumulation of the nanoparticles in the biofilm.
Keywords
SURVIVAL, biofilm, DIFFUSION, INFECTIONS, PENETRATION, HUMAN MUCUS, LIQUID FLOW, HETEROGENEOUS BIOFILMS, PSEUDOMONAS-AERUGINOSA, BURKHOLDERIA-CEPACIA, GRAM-POSITIVE BACTERIA, sputum, single-particle tracking, Pseudomonas aeruginosa, nanomedicine, diffusion, cystic fibrosis, confocal microscopy, Burkholderia multivorans, Burkholderia cepacia complex

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Citation

Please use this url to cite or link to this publication:

Chicago
Forier, Katrien, Anne-Sophie Messiaen, Koen Raemdonck, Hendrik Deschout, Joanna Rejman, Frans De Baets, Hans Nelis, et al. 2013. “Transport of Nanoparticles in Cystic Fibrosis Sputum and Bacterial Biofilms by Single-particle Tracking Microscopy.” Nanomedicine 8 (6): 935–949.
APA
Forier, K., Messiaen, A.-S., Raemdonck, K., Deschout, H., Rejman, J., De Baets, F., Nelis, H., et al. (2013). Transport of nanoparticles in cystic fibrosis sputum and bacterial biofilms by single-particle tracking microscopy. NANOMEDICINE, 8(6), 935–949.
Vancouver
1.
Forier K, Messiaen A-S, Raemdonck K, Deschout H, Rejman J, De Baets F, et al. Transport of nanoparticles in cystic fibrosis sputum and bacterial biofilms by single-particle tracking microscopy. NANOMEDICINE. 2013;8(6):935–49.
MLA
Forier, Katrien, Anne-Sophie Messiaen, Koen Raemdonck, et al. “Transport of Nanoparticles in Cystic Fibrosis Sputum and Bacterial Biofilms by Single-particle Tracking Microscopy.” NANOMEDICINE 8.6 (2013): 935–949. Print.
@article{3033622,
  abstract     = {Aim: The aim of this study was to evaluate the effect of the surface functionalization of model nanoparticles on their mobility in bacterial biofilms and cystic fibrosis sputum.
Materials \& methods: With single-particle tracking microscopy, the mobility of 0.1- and 0.2-mu m fluorescent polyethylene glycol (PEG) modified, carboxylate- and N,N-dimethylethylenediamine-modified polystyrene nanospheres were evaluated in fresh cystic fibrosis sputum, as well as Burkholderia multivorans and Pseudomonas aeruginosa biofilms.
Results: PEGylation increased the mobility of the particles in sputum and biofilms, while the charged nanospheres were strongly immobilized. However, the transport of the PEGylated nanoparticles was lower in sputum compared with biofilms. Furthermore, the particle transport showed heterogeneity in samples originating from different patients.
Conclusion: This study's data suggest that for future nanocarrier design it will be essential to combine PEGylation with a targeting moiety to ensure sufficient mobility in mucus and a better accumulation of the nanoparticles in the biofilm.},
  author       = {Forier, Katrien and Messiaen, Anne-Sophie and Raemdonck, Koen and Deschout, Hendrik and Rejman, Joanna and De Baets, Frans and Nelis, Hans and De Smedt, Stefaan and Demeester, Jo and Coenye, Tom and Braeckmans, Kevin},
  issn         = {1743-5889},
  journal      = {NANOMEDICINE},
  keyword      = {SURVIVAL,biofilm,DIFFUSION,INFECTIONS,PENETRATION,HUMAN MUCUS,LIQUID FLOW,HETEROGENEOUS BIOFILMS,PSEUDOMONAS-AERUGINOSA,BURKHOLDERIA-CEPACIA,GRAM-POSITIVE BACTERIA,sputum,single-particle tracking,Pseudomonas aeruginosa,nanomedicine,diffusion,cystic fibrosis,confocal microscopy,Burkholderia multivorans,Burkholderia cepacia complex},
  language     = {eng},
  number       = {6},
  pages        = {935--949},
  title        = {Transport of nanoparticles in cystic fibrosis sputum and bacterial biofilms by single-particle tracking microscopy},
  url          = {http://dx.doi.org/10.2217/NNM.12.129},
  volume       = {8},
  year         = {2013},
}

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