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Microarray analysis of the intestinal host response in Giardia duodenalis assemblage E infected calves

Leentje Dreesen UGent, Manuela Rinaldi UGent, Koen Chiers UGent, Robert Li, Thomas Geurden UGent, Wim Van Den Broeck UGent, Bruno Goddeeris UGent, Jozef Vercruysse UGent, Edwin Claerebout UGent and Peter Geldhof UGent (2012) PLOS ONE. 7(7).
abstract
Despite Giardia duodenalis being one of the most commonly found intestinal pathogens in humans and animals, little is known about the host-parasite interactions in its natural hosts. Therefore, the objective of this study was to investigate the intestinal response in calves following a G. duodenalis infection, using a bovine high-density oligo microarray to analyze global gene expression in the small intestine. The resulting microarray data suggested a decrease in inflammation, immune response, and immune cell migration in infected animals. These findings were examined in more detail by histological analyses combined with quantitative real-time PCR on a panel of cytokines. The transcription levels of IL-6, IL-8, IL-13, IL-17, and IFN-gamma showed a trend of being downregulated in the jejunum of infected animals compared to the negative controls,. No immune cell recruitment could be seen after infection, and no intestinal pathologies, such as villus shortening or increased levels of apoptosis. Possible regulators of this intestinal response are the nuclear peroxisome proliferator-activated receptors alpha (PPAR alpha), and gamma (PPAR gamma) and the enzyme adenosine deaminase (ADA), all for which an upregulated expression was found in the microarray and qRT-PCR analyses.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PROTOZOA, GENOTYPE, CATTLE, NORMALIZATION, INFLAMMATION, VARIANCE, IMMUNITY, LAMBLIA, GENE-EXPRESSION, EPITHELIAL-CELLS
journal title
PLOS ONE
PLoS One
volume
7
issue
7
article_number
e40985
pages
8 pages
Web of Science type
Article
Web of Science id
000306950200036
JCR category
MULTIDISCIPLINARY SCIENCES
JCR impact factor
3.73 (2012)
JCR rank
7/56 (2012)
JCR quartile
1 (2012)
ISSN
1932-6203
DOI
10.1371/journal.pone.0040985
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
3033516
handle
http://hdl.handle.net/1854/LU-3033516
date created
2012-10-23 16:22:41
date last changed
2012-10-25 10:41:41
@article{3033516,
  abstract     = {Despite Giardia duodenalis being one of the most commonly found intestinal pathogens in humans and animals, little is known about the host-parasite interactions in its natural hosts. Therefore, the objective of this study was to investigate the intestinal response in calves following a G. duodenalis infection, using a bovine high-density oligo microarray to analyze global gene expression in the small intestine. The resulting microarray data suggested a decrease in inflammation, immune response, and immune cell migration in infected animals. These findings were examined in more detail by histological analyses combined with quantitative real-time PCR on a panel of cytokines. The transcription levels of IL-6, IL-8, IL-13, IL-17, and IFN-gamma showed a trend of being downregulated in the jejunum of infected animals compared to the negative controls,. No immune cell recruitment could be seen after infection, and no intestinal pathologies, such as villus shortening or increased levels of apoptosis. Possible regulators of this intestinal response are the nuclear peroxisome proliferator-activated receptors alpha (PPAR alpha), and gamma (PPAR gamma) and the enzyme adenosine deaminase (ADA), all for which an upregulated expression was found in the microarray and qRT-PCR analyses.},
  articleno    = {e40985},
  author       = {Dreesen, Leentje and Rinaldi, Manuela and Chiers, Koen and Li, Robert and Geurden, Thomas and Van Den Broeck, Wim and Goddeeris, Bruno and Vercruysse, Jozef and Claerebout, Edwin and Geldhof, Peter},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keyword      = {PROTOZOA,GENOTYPE,CATTLE,NORMALIZATION,INFLAMMATION,VARIANCE,IMMUNITY,LAMBLIA,GENE-EXPRESSION,EPITHELIAL-CELLS},
  language     = {eng},
  number       = {7},
  pages        = {8},
  title        = {Microarray analysis of the intestinal host response in Giardia duodenalis assemblage E infected calves},
  url          = {http://dx.doi.org/10.1371/journal.pone.0040985},
  volume       = {7},
  year         = {2012},
}

Chicago
Dreesen, Leentje, Manuela Rinaldi, Koen Chiers, Robert Li, Thomas Geurden, Wim Van Den Broeck, Bruno Goddeeris, Jozef Vercruysse, Edwin Claerebout, and Peter Geldhof. 2012. “Microarray Analysis of the Intestinal Host Response in Giardia Duodenalis Assemblage E Infected Calves.” Plos One 7 (7).
APA
Dreesen, L., Rinaldi, M., Chiers, K., Li, R., Geurden, T., Van Den Broeck, W., Goddeeris, B., et al. (2012). Microarray analysis of the intestinal host response in Giardia duodenalis assemblage E infected calves. PLOS ONE, 7(7).
Vancouver
1.
Dreesen L, Rinaldi M, Chiers K, Li R, Geurden T, Van Den Broeck W, et al. Microarray analysis of the intestinal host response in Giardia duodenalis assemblage E infected calves. PLOS ONE. 2012;7(7).
MLA
Dreesen, Leentje, Manuela Rinaldi, Koen Chiers, et al. “Microarray Analysis of the Intestinal Host Response in Giardia Duodenalis Assemblage E Infected Calves.” PLOS ONE 7.7 (2012): n. pag. Print.