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Evaluation of the automated haematology analyser Sysmex NE-8000

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Abstract
The automated haematology analyser Sysmex NE-8000 was compared with the Technicon H-1, the automated analyser routinely used in our laboratory, and with manual cell differentiation results. One hundred and seventy samples from the daily routine workload, comprising specimens from healthy adults and patients with various ailments, were analysed on the Sysmex NE-8000 and the Technicon H-1. A manual-400 leukocyte differential count was performed on each specimen. Comparison of the results from the two blood cell counters showed good correlation (r > 0.9) for the white blood cell count, haemoglobin, haematocrit and platelet count. For the red blood cell count and mean cellular volume, the correlation coefficients were greater than 0.8. In the leukocyte differential count, Sysmex NE-8000 and Techicon H-1 showed good correlations for the neutrophil (r = 0.953), lymphocyte (r = 0.763), and eosinophil counts (r = 0.904). Correlation coefficients were very low for monocyte (r = 0.130) and basophil counts (r = 0.006). Correlation between the manual-400 method and the electronic leukocyte differential count showed similar results. Two hundred and twenty six normal and abnormal samples were compared with respect to morphology flagging with the two analysers, using the manual differentiation as the reference method. The abnormal specimens were representative of the range of leukocyte abnormalities seen in our laboratory. Sensitivity for detecting blasts was equal for both analysers. Sysmex NE-8000 was much more sensitive for detecting immature granulocytes than Technicon H-1. Low ranges of atypical lymphocytes were missed by Sysmex NE-8000. Left shift was also frequently missed. During the evaluation period, Sysmex NE-8000 was very easy to handle and no instrument malfunctions were met. The Sysmex NE-8000 is well suited for routine blood cell analysis and is a valuable tool for the diagnosis and screening of various haematological abnormalities.
Keywords
DIFFERENTIATION, COUNTS, BLOOD

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Chicago
Devreese, Katrien, ERIK DE LOGI, Christiane Francart, B Heyndrickx, Jan Philippé, and Geert Leroux-Roels. 1991. “Evaluation of the Automated Haematology Analyser Sysmex NE-8000.” European Journal of Clinical Chemistry and Clinical Biochemistry 29 (5): 339–345.
APA
Devreese, K., DE LOGI, E., Francart, C., Heyndrickx, B., Philippé, J., & Leroux-Roels, G. (1991). Evaluation of the automated haematology analyser Sysmex NE-8000. EUROPEAN JOURNAL OF CLINICAL CHEMISTRY AND CLINICAL BIOCHEMISTRY, 29(5), 339–345.
Vancouver
1.
Devreese K, DE LOGI E, Francart C, Heyndrickx B, Philippé J, Leroux-Roels G. Evaluation of the automated haematology analyser Sysmex NE-8000. EUROPEAN JOURNAL OF CLINICAL CHEMISTRY AND CLINICAL BIOCHEMISTRY. 1991;29(5):339–45.
MLA
Devreese, Katrien, ERIK DE LOGI, Christiane Francart, et al. “Evaluation of the Automated Haematology Analyser Sysmex NE-8000.” EUROPEAN JOURNAL OF CLINICAL CHEMISTRY AND CLINICAL BIOCHEMISTRY 29.5 (1991): 339–345. Print.
@article{3032994,
  abstract     = {The automated haematology analyser Sysmex NE-8000 was compared with the Technicon H-1, the automated analyser routinely used in our laboratory, and with manual cell differentiation results. 
One hundred and seventy samples from the daily routine workload, comprising specimens from healthy adults and patients with various ailments, were analysed on the Sysmex NE-8000 and the Technicon H-1. A manual-400 leukocyte differential count was performed on each specimen. Comparison of the results from the two blood cell counters showed good correlation (r {\textrangle} 0.9) for the white blood cell count, haemoglobin, haematocrit and platelet count. For the red blood cell count and mean cellular volume, the correlation coefficients were greater than 0.8. In the leukocyte differential count, Sysmex NE-8000 and Techicon H-1 showed good correlations for the neutrophil (r = 0.953), lymphocyte (r = 0.763), and eosinophil counts (r = 0.904). Correlation coefficients were very low for monocyte (r = 0.130) and basophil counts (r = 0.006). Correlation between the manual-400 method and the electronic leukocyte differential count showed similar results. 
Two hundred and twenty six normal and abnormal samples were compared with respect to morphology flagging with the two analysers, using the manual differentiation as the reference method. The abnormal specimens were representative of the range of leukocyte abnormalities seen in our laboratory. Sensitivity for detecting blasts was equal for both analysers. Sysmex NE-8000 was much more sensitive for detecting immature granulocytes than Technicon H-1. Low ranges of atypical lymphocytes were missed by Sysmex NE-8000. Left shift was also frequently missed. 
During the evaluation period, Sysmex NE-8000 was very easy to handle and no instrument malfunctions were met. The Sysmex NE-8000 is well suited for routine blood cell analysis and is a valuable tool for the diagnosis and screening of various haematological abnormalities.},
  author       = {Devreese, Katrien and DE LOGI, ERIK and Francart, Christiane and Heyndrickx, B and Philipp{\'e}, Jan and Leroux-Roels, Geert},
  issn         = {0939-4974},
  journal      = {EUROPEAN JOURNAL OF CLINICAL CHEMISTRY AND CLINICAL BIOCHEMISTRY},
  keyword      = {DIFFERENTIATION,COUNTS,BLOOD},
  language     = {eng},
  number       = {5},
  pages        = {339--345},
  title        = {Evaluation of the automated haematology analyser Sysmex NE-8000},
  volume       = {29},
  year         = {1991},
}