Ghent University Academic Bibliography

Advanced

Production of a subunit vaccine candidate against porcine post-weaning diarrhea in high-biomass transplastomic tobacco

Igor Kolotilin, Angelo Kaldis, Bert Devriendt UGent, Jussi Joensuu, Eric Cox UGent and Rima Menassa (2012) PLOS ONE. 7(8).
abstract
Post-weaning diarrhea (PWD) in piglets is a major problem in piggeries worldwide and results in severe economic losses. Infection with Enterotoxigenic Escherichia coli (ETEC) is the key culprit for the PWD disease. F4 fimbriae of ETEC are highly stable proteinaceous polymers, mainly composed of the major structural subunit FaeG, with a capacity to evoke mucosal immune responses, thus demonstrating a potential to act as an oral vaccine against ETEC-induced porcine PWD. In this study we used a transplastomic approach in tobacco to produce a recombinant variant of the FaeG protein, rFaeG(ntd/dsc), engineered for expression as a stable monomer by N-terminal deletion and donor strand-complementation (ntd/dsc). The generated transplastomic tobacco plants accumulated up to 2.0 g rFaeG(ntd/dsc) per 1 kg fresh leaf tissue (more than 1% of dry leaf tissue) and showed normal phenotype indistinguishable from wild type untransformed plants. We determined that chloroplast-produced rFaeG(ntd/dsc) protein retained the key properties of an oral vaccine, i.e. binding to porcine intestinal F4 receptors (F4R), and inhibition of the F4-possessing (F4+) ETEC attachment to F4R. Additionally, the plant biomass matrix was shown to delay degradation of the chloroplast-produced rFaeG(ntd/dsc) in gastrointestinal conditions, demonstrating a potential to function as a shelter-vehicle for vaccine delivery. These results suggest that transplastomic plants expressing the rFaeG(ntd/dsc) protein could be used for production and, possibly, delivery of an oral vaccine against porcine F4+ ETEC infections. Our findings therefore present a feasible approach for developing an oral vaccination strategy against porcine PWD.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
HIGH-LEVEL EXPRESSION, PSBA MESSENGER-RNA, F4 K88 FIMBRIAE, TRANSGENIC TOBACCO, ORAL IMMUNIZATION, MAJOR SUBUNIT, CHLOROPLAST TRANSFORMATION, NUCLEOTIDE-SEQUENCE, PROTEIN EXPRESSION, ENTEROTOXIGENIC ESCHERICHIA-COLI
journal title
PLOS ONE
PLoS One
volume
7
issue
8
article_number
e42405
pages
12 pages
Web of Science type
Article
Web of Science id
000307284100081
JCR category
MULTIDISCIPLINARY SCIENCES
JCR impact factor
3.73 (2012)
JCR rank
7/56 (2012)
JCR quartile
1 (2012)
ISSN
1932-6203
DOI
10.1371/journal.pone.0042405
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
3032788
handle
http://hdl.handle.net/1854/LU-3032788
date created
2012-10-22 15:28:21
date last changed
2012-10-23 10:09:06
@article{3032788,
  abstract     = {Post-weaning diarrhea (PWD) in piglets is a major problem in piggeries worldwide and results in severe economic losses. Infection with Enterotoxigenic Escherichia coli (ETEC) is the key culprit for the PWD disease. F4 fimbriae of ETEC are highly stable proteinaceous polymers, mainly composed of the major structural subunit FaeG, with a capacity to evoke mucosal immune responses, thus demonstrating a potential to act as an oral vaccine against ETEC-induced porcine PWD. In this study we used a transplastomic approach in tobacco to produce a recombinant variant of the FaeG protein, rFaeG(ntd/dsc), engineered for expression as a stable monomer by N-terminal deletion and donor strand-complementation (ntd/dsc). The generated transplastomic tobacco plants accumulated up to 2.0 g rFaeG(ntd/dsc) per 1 kg fresh leaf tissue (more than 1\% of dry leaf tissue) and showed normal phenotype indistinguishable from wild type untransformed plants. We determined that chloroplast-produced rFaeG(ntd/dsc) protein retained the key properties of an oral vaccine, i.e. binding to porcine intestinal F4 receptors (F4R), and inhibition of the F4-possessing (F4+) ETEC attachment to F4R. Additionally, the plant biomass matrix was shown to delay degradation of the chloroplast-produced rFaeG(ntd/dsc) in gastrointestinal conditions, demonstrating a potential to function as a shelter-vehicle for vaccine delivery. These results suggest that transplastomic plants expressing the rFaeG(ntd/dsc) protein could be used for production and, possibly, delivery of an oral vaccine against porcine F4+ ETEC infections. Our findings therefore present a feasible approach for developing an oral vaccination strategy against porcine PWD.},
  articleno    = {e42405},
  author       = {Kolotilin, Igor and Kaldis, Angelo and Devriendt, Bert and Joensuu, Jussi and Cox, Eric and Menassa, Rima},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keyword      = {HIGH-LEVEL EXPRESSION,PSBA MESSENGER-RNA,F4 K88 FIMBRIAE,TRANSGENIC TOBACCO,ORAL IMMUNIZATION,MAJOR SUBUNIT,CHLOROPLAST TRANSFORMATION,NUCLEOTIDE-SEQUENCE,PROTEIN EXPRESSION,ENTEROTOXIGENIC ESCHERICHIA-COLI},
  language     = {eng},
  number       = {8},
  pages        = {12},
  title        = {Production of a subunit vaccine candidate against porcine post-weaning diarrhea in high-biomass transplastomic tobacco},
  url          = {http://dx.doi.org/10.1371/journal.pone.0042405},
  volume       = {7},
  year         = {2012},
}

Chicago
Kolotilin, Igor, Angelo Kaldis, Bert Devriendt, Jussi Joensuu, Eric Cox, and Rima Menassa. 2012. “Production of a Subunit Vaccine Candidate Against Porcine Post-weaning Diarrhea in High-biomass Transplastomic Tobacco.” Plos One 7 (8).
APA
Kolotilin, I., Kaldis, A., Devriendt, B., Joensuu, J., Cox, E., & Menassa, R. (2012). Production of a subunit vaccine candidate against porcine post-weaning diarrhea in high-biomass transplastomic tobacco. PLOS ONE, 7(8).
Vancouver
1.
Kolotilin I, Kaldis A, Devriendt B, Joensuu J, Cox E, Menassa R. Production of a subunit vaccine candidate against porcine post-weaning diarrhea in high-biomass transplastomic tobacco. PLOS ONE. 2012;7(8).
MLA
Kolotilin, Igor, Angelo Kaldis, Bert Devriendt, et al. “Production of a Subunit Vaccine Candidate Against Porcine Post-weaning Diarrhea in High-biomass Transplastomic Tobacco.” PLOS ONE 7.8 (2012): n. pag. Print.