Ghent University Academic Bibliography

Advanced

Pathogen-group specific association between CXCR1 polymorphisms and subclinical mastitis in dairy heifers

Joren Verbeke UGent, Sofie Piepers UGent, Luc Peelman UGent, Mario Van Poucke UGent and Sarne De Vliegher UGent (2012) JOURNAL OF DAIRY RESEARCH. 79(3). p.341-351
abstract
The chemokine (C-X-C motif) receptor 1 (CXCR1) gene encodes the homonymous receptor for interleukin 8 (IL8) on polymorphonuclear neutrophilic leucocytes (PMNL). Binding causes migration from blood to milk, activation and prolonged survival of PMNL, a crucial process in the innate immune defence of the bovine mammary gland against invading mastitis-causing pathogens. The main objective of this study was to screen the entire coding region of the CXCR1 gene for polymorphisms and to analyse their association with udder health of dairy heifers. One-hundred-and-forty Belgian Holstein heifers originating from 20 commercial dairy farms were genotyped by DNA sequencing. Detailed phenotypic data on udder health was available including quarter bacteriological culture results and somatic cell count (SCC) in early lactation and composite milk SCC during first lactation. In total, 16 polymorphisms (including 8 missense mutations) were detected. Polymorphism c.980A>G was associated with pathogen-group specific IMI: heifers with genotype AG were less likely to have an IMI due to major mastitis pathogens compared with heifers with genotype GG but did not have less IMI by coagulase-negative staphylococci, so-called minor pathogens. CXCR1 genotype was neither associated with quarter SCC in early lactation nor with composite SCC during lactation. Although mastitis susceptibility is influenced by many factors, some genetic polymorphisms potentially have major effects on udder health of heifers, as was shown here. These results trigger us to further study the relationship between CXCR1 polymorphisms and mastitis susceptibility in both observational and experimental trials.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
CXCR1 polymorphisms, SINGLE NUCLEOTIDE POLYMORPHISMS, heifer mastitis, polymorphonuclear neutrophilic leucocytes, CATTLE, UDDER HEALTH, BOVINE INTERLEUKIN-8, RISK-FACTORS, EARLY LACTATION, INTRAMAMMARY INFECTIONS, CLINICAL MASTITIS, MILK-PRODUCTION, SOMATIC-CELL SCORE
journal title
JOURNAL OF DAIRY RESEARCH
J. Dairy Res.
volume
79
issue
3
pages
341 - 351
Web of Science type
Article
Web of Science id
000307181600012
JCR category
AGRICULTURE, DAIRY & ANIMAL SCIENCE
JCR impact factor
1.373 (2012)
JCR rank
14/54 (2012)
JCR quartile
2 (2012)
ISSN
0022-0299
DOI
10.1017/S0022029912000349
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
3031313
handle
http://hdl.handle.net/1854/LU-3031313
date created
2012-10-18 14:53:50
date last changed
2012-10-23 10:04:31
@article{3031313,
  abstract     = {The chemokine (C-X-C motif) receptor 1 (CXCR1) gene encodes the homonymous receptor for interleukin 8 (IL8) on polymorphonuclear neutrophilic leucocytes (PMNL). Binding causes migration from blood to milk, activation and prolonged survival of PMNL, a crucial process in the innate immune defence of the bovine mammary gland against invading mastitis-causing pathogens. The main objective of this study was to screen the entire coding region of the CXCR1 gene for polymorphisms and to analyse their association with udder health of dairy heifers. One-hundred-and-forty Belgian Holstein heifers originating from 20 commercial dairy farms were genotyped by DNA sequencing. Detailed phenotypic data on udder health was available including quarter bacteriological culture results and somatic cell count (SCC) in early lactation and composite milk SCC during first lactation. In total, 16 polymorphisms (including 8 missense mutations) were detected. Polymorphism c.980A{\textrangle}G was associated with pathogen-group specific IMI: heifers with genotype AG were less likely to have an IMI due to major mastitis pathogens compared with heifers with genotype GG but did not have less IMI by coagulase-negative staphylococci, so-called minor pathogens. CXCR1 genotype was neither associated with quarter SCC in early lactation nor with composite SCC during lactation. Although mastitis susceptibility is influenced by many factors, some genetic polymorphisms potentially have major effects on udder health of heifers, as was shown here. These results trigger us to further study the relationship between CXCR1 polymorphisms and mastitis susceptibility in both observational and experimental trials.},
  author       = {Verbeke, Joren and Piepers, Sofie and Peelman, Luc and Van Poucke, Mario and De Vliegher, Sarne},
  issn         = {0022-0299},
  journal      = {JOURNAL OF DAIRY RESEARCH},
  keyword      = {CXCR1 polymorphisms,SINGLE NUCLEOTIDE POLYMORPHISMS,heifer mastitis,polymorphonuclear neutrophilic leucocytes,CATTLE,UDDER HEALTH,BOVINE INTERLEUKIN-8,RISK-FACTORS,EARLY LACTATION,INTRAMAMMARY INFECTIONS,CLINICAL MASTITIS,MILK-PRODUCTION,SOMATIC-CELL SCORE},
  language     = {eng},
  number       = {3},
  pages        = {341--351},
  title        = {Pathogen-group specific association between CXCR1 polymorphisms and subclinical mastitis in dairy heifers},
  url          = {http://dx.doi.org/10.1017/S0022029912000349},
  volume       = {79},
  year         = {2012},
}

Chicago
Verbeke, Joren, Sofie Piepers, Luc Peelman, Mario Van Poucke, and Sarne De Vliegher. 2012. “Pathogen-group Specific Association Between CXCR1 Polymorphisms and Subclinical Mastitis in Dairy Heifers.” Journal of Dairy Research 79 (3): 341–351.
APA
Verbeke, J., Piepers, S., Peelman, L., Van Poucke, M., & De Vliegher, S. (2012). Pathogen-group specific association between CXCR1 polymorphisms and subclinical mastitis in dairy heifers. JOURNAL OF DAIRY RESEARCH, 79(3), 341–351.
Vancouver
1.
Verbeke J, Piepers S, Peelman L, Van Poucke M, De Vliegher S. Pathogen-group specific association between CXCR1 polymorphisms and subclinical mastitis in dairy heifers. JOURNAL OF DAIRY RESEARCH. 2012;79(3):341–51.
MLA
Verbeke, Joren, Sofie Piepers, Luc Peelman, et al. “Pathogen-group Specific Association Between CXCR1 Polymorphisms and Subclinical Mastitis in Dairy Heifers.” JOURNAL OF DAIRY RESEARCH 79.3 (2012): 341–351. Print.