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Immunization with the immunodominant Helicobacter suis urease subunit B induces partial protection against H. suis infection in a mouse model

Miet Vermoote UGent, Katleen Van Steendam UGent, Bram Flahou UGent, Annemieke Smet UGent, Frank Pasmans UGent, Pieter Glibert UGent, Richard Ducatelle UGent, Dieter Deforce UGent and Freddy Haesebrouck UGent (2012) VETERINARY RESEARCH. 43.
abstract
Helicobacter (H.) suis is a porcine and human gastric pathogen. Previous studies in mice showed that an H. suis infection does not result in protective immunity, whereas immunization with H. suis whole-cell lysate (lysate) protects against a subsequent experimental infection. Therefore, two-dimensional gel electrophoresis of H. suis proteins was performed followed by immunoblotting with pooled sera from H. suis- infected mice or mice immunized with lysate. Weak reactivity against H. suis proteins was observed in post-infection sera. Sera from lysate-immunized mice, however, showed immunoreactivity against a total of 19 protein spots which were identified using LC-MS/MS. The H. suis urease subunit B (UreB) showed most pronounced reactivity against sera from lysate-immunized mice and was not detected with sera from infected mice. None of the pooled sera detected H. suis neutrophil-activating protein A (NapA). The protective efficacy of intranasal vaccination of BALB/c mice with H. suis UreB and NapA, both recombinantly expressed in Escherichia coli (rUreB and rNapA, respectively), was compared with that of H. suis lysate. All vaccines contained choleratoxin as adjuvant. Immunization of mice with rUreB and lysate induced a significant reduction of H. suis colonization compared to non-vaccinated H. suis-infected controls, whereas rNapA had no significant protective effect. Probably, a combination of local Th1 and Th17 responses, complemented by antibody responses play a role in the protective immunity against H. suis infections.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
journal title
VETERINARY RESEARCH
Vet. Res.
volume
43
article_number
72
pages
13 pages
Web of Science type
Article
Web of Science id
000314020100001
JCR category
VETERINARY SCIENCES
JCR impact factor
3.426 (2012)
JCR rank
1/142 (2012)
JCR quartile
1 (2012)
ISSN
0928-4249
DOI
10.1186/1297-9716-43-72
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
3029756
handle
http://hdl.handle.net/1854/LU-3029756
date created
2012-10-16 14:55:06
date last changed
2013-07-05 15:28:14
@article{3029756,
  abstract     = {Helicobacter (H.) suis is a porcine and human gastric pathogen. Previous studies in mice showed that an H. suis infection does not result in protective immunity, whereas immunization with H. suis whole-cell lysate (lysate) protects against a subsequent experimental infection. Therefore, two-dimensional gel electrophoresis of H. suis proteins was performed followed by immunoblotting with pooled sera from H. suis- infected mice or mice immunized with lysate. Weak reactivity against H. suis proteins was observed in post-infection sera. Sera from lysate-immunized mice, however, showed immunoreactivity against a total of 19 protein spots which were identified using LC-MS/MS. The H. suis urease subunit B (UreB) showed most pronounced reactivity against sera from lysate-immunized mice and was not detected with sera from infected mice. None of the pooled sera detected H. suis neutrophil-activating protein A (NapA). The protective efficacy of intranasal vaccination of BALB/c mice with H. suis UreB and NapA, both recombinantly expressed in Escherichia coli (rUreB and rNapA, respectively), was compared with that of H. suis lysate. All vaccines contained choleratoxin as adjuvant. Immunization of mice with rUreB and lysate induced a significant reduction of H. suis colonization compared to non-vaccinated H. suis-infected controls, whereas rNapA had no significant protective effect. Probably, a combination of local Th1 and Th17 responses, complemented by antibody responses play a role in the protective immunity against H. suis infections.},
  articleno    = {72},
  author       = {Vermoote, Miet and Van Steendam, Katleen and Flahou, Bram and Smet, Annemieke and Pasmans, Frank and Glibert, Pieter and Ducatelle, Richard and Deforce, Dieter and Haesebrouck, Freddy},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  language     = {eng},
  pages        = {13},
  title        = {Immunization with the immunodominant Helicobacter suis urease subunit B induces partial protection against H. suis infection in a mouse model},
  url          = {http://dx.doi.org/10.1186/1297-9716-43-72},
  volume       = {43},
  year         = {2012},
}

Chicago
Vermoote, Miet, Katleen Van Steendam, Bram Flahou, Annemieke Smet, Frank Pasmans, Pieter Glibert, Richard Ducatelle, Dieter Deforce, and Freddy Haesebrouck. 2012. “Immunization with the Immunodominant Helicobacter Suis Urease Subunit B Induces Partial Protection Against H. Suis Infection in a Mouse Model.” Veterinary Research 43.
APA
Vermoote, M., Van Steendam, K., Flahou, B., Smet, A., Pasmans, F., Glibert, P., Ducatelle, R., et al. (2012). Immunization with the immunodominant Helicobacter suis urease subunit B induces partial protection against H. suis infection in a mouse model. VETERINARY RESEARCH, 43.
Vancouver
1.
Vermoote M, Van Steendam K, Flahou B, Smet A, Pasmans F, Glibert P, et al. Immunization with the immunodominant Helicobacter suis urease subunit B induces partial protection against H. suis infection in a mouse model. VETERINARY RESEARCH. 2012;43.
MLA
Vermoote, Miet, Katleen Van Steendam, Bram Flahou, et al. “Immunization with the Immunodominant Helicobacter Suis Urease Subunit B Induces Partial Protection Against H. Suis Infection in a Mouse Model.” VETERINARY RESEARCH 43 (2012): n. pag. Print.