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Short cigarette smoke exposure facilitates sensitisation and asthma development in mice

Ellen Lanckacker (UGent) , Kurt Tournoy (UGent) , Hamida Hammad (UGent) , Gabriële Holtappels (UGent) , Bart Lambrecht (UGent) , Guy Joos (UGent) and Tania Maes (UGent)
(2013) EUROPEAN RESPIRATORY JOURNAL. 41(5). p.1189-1199
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Abstract
Epidemiological studies indicate that cigarette smoke (CS) exposure is a risk factor for increased sensitization and asthma development. The aim of the study was to examine the impact of CS on sensitization and allergic airway inflammation, in response to a low dose of house dust mite (HDM), and to obtain potential mechanistic insights.Mice were exposed to low doses of HDM extract combined with air or CS exposure, either during allergen sensitization or during development of allergic airway disease.Mice concomitantly exposed to low dose HDM, combined with CS for 3 weeks, demonstrated an asthmatic phenotype with significantly increased airway eosinophilia, goblet cell metaplasia, airway hyperresponsiveness and a rise in HDM-specific serum IgG1, compared to sole HDM or CS exposure. In addition, short CS inhalation, during the initial contact with HDM allergens, was sufficient to facilitate sensitization and development of a complete asthmatic phenotype after rechallenge with HDM. Mechanistically, short CS exposure amplified DC-mediated transport of FITC-labelled HDM allergens to the intrathoracic lymph nodes and generated a local Th2 response.Short CS exposure is sufficient to facilitate allergic sensitization and the development of low dose HDM-induced allergic asthma, possibly through affecting dendritic cell function.
Keywords
dendritic cell, HOUSE-DUST MITE, environmental pollutants, murine, Airway allergy, ALLERGIC AIRWAY INFLAMMATION, DERMATOPHAGOIDES-PTERONYSSINUS, DENDRITIC CELLS, CARBOXYHEMOGLOBIN LEVELS, ENVIRONMENTAL TOBACCO-SMOKE, INDUCED PULMONARY INFLAMMATION, EPITHELIAL-CELLS, LUNG-FUNCTION, MOUSE MODEL

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MLA
Lanckacker, Ellen, Kurt Tournoy, Hamida Hammad, et al. “Short Cigarette Smoke Exposure Facilitates Sensitisation and Asthma Development in Mice.” EUROPEAN RESPIRATORY JOURNAL 41.5 (2013): 1189–1199. Print.
APA
Lanckacker, E., Tournoy, K., Hammad, H., Holtappels, G., Lambrecht, B., Joos, G., & Maes, T. (2013). Short cigarette smoke exposure facilitates sensitisation and asthma development in mice. EUROPEAN RESPIRATORY JOURNAL, 41(5), 1189–1199.
Chicago author-date
Lanckacker, Ellen, Kurt Tournoy, Hamida Hammad, Gabriële Holtappels, Bart Lambrecht, Guy Joos, and Tania Maes. 2013. “Short Cigarette Smoke Exposure Facilitates Sensitisation and Asthma Development in Mice.” European Respiratory Journal 41 (5): 1189–1199.
Chicago author-date (all authors)
Lanckacker, Ellen, Kurt Tournoy, Hamida Hammad, Gabriële Holtappels, Bart Lambrecht, Guy Joos, and Tania Maes. 2013. “Short Cigarette Smoke Exposure Facilitates Sensitisation and Asthma Development in Mice.” European Respiratory Journal 41 (5): 1189–1199.
Vancouver
1.
Lanckacker E, Tournoy K, Hammad H, Holtappels G, Lambrecht B, Joos G, et al. Short cigarette smoke exposure facilitates sensitisation and asthma development in mice. EUROPEAN RESPIRATORY JOURNAL. 2013;41(5):1189–99.
IEEE
[1]
E. Lanckacker et al., “Short cigarette smoke exposure facilitates sensitisation and asthma development in mice,” EUROPEAN RESPIRATORY JOURNAL, vol. 41, no. 5, pp. 1189–1199, 2013.
@article{3028966,
  abstract     = {Epidemiological studies indicate that cigarette smoke (CS) exposure is a risk factor for increased sensitization and asthma development. The aim of the study was to examine the impact of CS on sensitization and allergic airway inflammation, in response to a low dose of house dust mite (HDM), and to obtain potential mechanistic insights.Mice were exposed to low doses of HDM extract combined with air or CS exposure, either during allergen sensitization or during development of allergic airway disease.Mice concomitantly exposed to low dose HDM, combined with CS for 3 weeks, demonstrated an asthmatic phenotype with significantly increased airway eosinophilia, goblet cell metaplasia, airway hyperresponsiveness and a rise in HDM-specific serum IgG1, compared to sole HDM or CS exposure. In addition, short CS inhalation, during the initial contact with HDM allergens, was sufficient to facilitate sensitization and development of a complete asthmatic phenotype after rechallenge with HDM. Mechanistically, short CS exposure amplified DC-mediated transport of FITC-labelled HDM allergens to the intrathoracic lymph nodes and generated a local Th2 response.Short CS exposure is sufficient to facilitate allergic sensitization and the development of low dose HDM-induced allergic asthma, possibly through affecting dendritic cell function.},
  author       = {Lanckacker, Ellen and Tournoy, Kurt and Hammad, Hamida and Holtappels, Gabriële and Lambrecht, Bart and Joos, Guy and Maes, Tania},
  issn         = {0903-1936},
  journal      = {EUROPEAN RESPIRATORY JOURNAL},
  keywords     = {dendritic cell,HOUSE-DUST MITE,environmental pollutants,murine,Airway allergy,ALLERGIC AIRWAY INFLAMMATION,DERMATOPHAGOIDES-PTERONYSSINUS,DENDRITIC CELLS,CARBOXYHEMOGLOBIN LEVELS,ENVIRONMENTAL TOBACCO-SMOKE,INDUCED PULMONARY INFLAMMATION,EPITHELIAL-CELLS,LUNG-FUNCTION,MOUSE MODEL},
  language     = {eng},
  number       = {5},
  pages        = {1189--1199},
  title        = {Short cigarette smoke exposure facilitates sensitisation and asthma development in mice},
  url          = {http://dx.doi.org/10.1183/09031936.00096612},
  volume       = {41},
  year         = {2013},
}

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