Ghent University Academic Bibliography

Advanced

Localization and control of expression of Nt-Syr1, a tobacco snare protein

Barbara Leyman UGent, Danny Geelen UGent and Michael R Blatt (2000) PLANT JOURNAL. 24(3). p.369-381
abstract
Syntaxins and other SNARE proteins are crucial for intracellular vesicle trafficking, fusion and secretion. Previously, we isolated the syntaxin-related protein Nt-Syr1 from Nicotiana in a screen for ABA-related signalling elements, and demonstrated its role in determining the ABA sensitivity of stomatal guard cells. Because the location and expression of SNAREs are often important clues to their functioning, we have examined the distribution and stimulus-dependent expression of Nt-Syr1 between tissues, as well as its location within the cell, using antisera raised against purified recombinant peptides corresponding to overlapping cytosolic domains of Nt-Syr1. The Nt-Syr1 epitope was strongly represented in roots and to lesser extents in stems, leaves and flowers of well-watered plants. Biochemical analysis and examination of immunogold labelling under the electron microscope indicated Nt-Syr1 to be located primarily at the plasma membrane. Expression of the protein in leaves and to a lesser extent in flowers and stems was transiently enhanced by ABA, but not bit auxin, kinetin or gibberellic acid. Expression in leaves was promoted by salt stress and wounding, but not by cold. By contrast, Nt-Syr1 levels in the root were unaffected by ABA. In the leaves, enhanced expression of Nt-Syr1 by salt stress was not observed in abal mutant Nicotiana, which is deficient in ABA synthesis, and in plants carrying the Arabidopsis abi1 transgene that suppresses a number of ABA-evoked responses in these plants. However, an enhanced expression in response to wounding was observed, even in the mutant backgrounds. We conclude that Nt-Syr1 expression at the plasma membrane is important for its function and is subject to control by parallel, stress-related signalling pathways, both dependent on and independent of ABA. Nt-Syr1 may be associated with additional functions, especially in the roots, that are unrelated to ABA or stress responses in the plant.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
syntaxin, Nicotiana, abiotic stress (salt, wounding), ABA, exocytosis, plasma membrane, ABSCISIC-ACID, ARABIDOPSIS-THALIANA, SIGNAL-TRANSDUCTION, MEMBRANE-FUSION, GUARD-CELLS, NICOTIANA-PLUMBAGINIFOLIA, PLASMA-MEMBRANE, ANION CHANNELS, GOLGI-COMPLEX, K+-CHANNELS
journal title
PLANT JOURNAL
Plant J.
volume
24
issue
3
pages
369 - 381
Web of Science type
Article
Web of Science id
000165518700009
ISSN
0960-7412
DOI
10.1046/j.1365-313x.2000.00886.x
language
English
UGent publication?
yes
classification
A1
additional info
the first two authors contributed equally to the paper
copyright statement
I have transferred the copyright for this publication to the publisher
id
302804
handle
http://hdl.handle.net/1854/LU-302804
date created
2005-05-02 11:14:00
date last changed
2013-10-02 12:26:38
@article{302804,
  abstract     = {Syntaxins and other SNARE proteins are crucial for intracellular vesicle trafficking, fusion and secretion. Previously, we isolated the syntaxin-related protein Nt-Syr1 from Nicotiana in a screen for ABA-related signalling elements, and demonstrated its role in determining the ABA sensitivity of stomatal guard cells. Because the location and expression of SNAREs are often important clues to their functioning, we have examined the distribution and stimulus-dependent expression of Nt-Syr1 between tissues, as well as its location within the cell, using antisera raised against purified recombinant peptides corresponding to overlapping cytosolic domains of Nt-Syr1. The Nt-Syr1 epitope was strongly represented in roots and to lesser extents in stems, leaves and flowers of well-watered plants. Biochemical analysis and examination of immunogold labelling under the electron microscope indicated Nt-Syr1 to be located primarily at the plasma membrane. Expression of the protein in leaves and to a lesser extent in flowers and stems was transiently enhanced by ABA, but not bit auxin, kinetin or gibberellic acid. Expression in leaves was promoted by salt stress and wounding, but not by cold. By contrast, Nt-Syr1 levels in the root were unaffected by ABA. In the leaves, enhanced expression of Nt-Syr1 by salt stress was not observed in abal mutant Nicotiana, which is deficient in ABA synthesis, and in plants carrying the Arabidopsis abi1 transgene that suppresses a number of ABA-evoked responses in these plants. However, an enhanced expression in response to wounding was observed, even in the mutant backgrounds. We conclude that Nt-Syr1 expression at the plasma membrane is important for its function and is subject to control by parallel, stress-related signalling pathways, both dependent on and independent of ABA. Nt-Syr1 may be associated with additional functions, especially in the roots, that are unrelated to ABA or stress responses in the plant.},
  author       = {Leyman, Barbara and Geelen, Danny and Blatt, Michael R},
  issn         = {0960-7412},
  journal      = {PLANT JOURNAL},
  keyword      = {syntaxin,Nicotiana,abiotic stress (salt,wounding),ABA,exocytosis,plasma membrane,ABSCISIC-ACID,ARABIDOPSIS-THALIANA,SIGNAL-TRANSDUCTION,MEMBRANE-FUSION,GUARD-CELLS,NICOTIANA-PLUMBAGINIFOLIA,PLASMA-MEMBRANE,ANION CHANNELS,GOLGI-COMPLEX,K+-CHANNELS},
  language     = {eng},
  number       = {3},
  pages        = {369--381},
  title        = {Localization and control of expression of Nt-Syr1, a tobacco snare protein},
  url          = {http://dx.doi.org/10.1046/j.1365-313x.2000.00886.x},
  volume       = {24},
  year         = {2000},
}

Chicago
Leyman, Barbara, Danny Geelen, and Michael R Blatt. 2000. “Localization and Control of Expression of Nt-Syr1, a Tobacco Snare Protein.” Plant Journal 24 (3): 369–381.
APA
Leyman, Barbara, Geelen, D., & Blatt, M. R. (2000). Localization and control of expression of Nt-Syr1, a tobacco snare protein. PLANT JOURNAL, 24(3), 369–381.
Vancouver
1.
Leyman B, Geelen D, Blatt MR. Localization and control of expression of Nt-Syr1, a tobacco snare protein. PLANT JOURNAL. 2000;24(3):369–81.
MLA
Leyman, Barbara, Danny Geelen, and Michael R Blatt. “Localization and Control of Expression of Nt-Syr1, a Tobacco Snare Protein.” PLANT JOURNAL 24.3 (2000): 369–381. Print.