Advanced search
1 file | 5.04 MB

Improving the glycosylation activity of cellobiose/cellodextrin phosphorylase through enzyme engineering

Hai Giang Tran (UGent)
(2012)
Author
Promoter
(UGent) and (UGent)
Organization
Abstract
This PhD project is part of a larger project that aims to develop a generic technology for the biocatalytic glycosylation of small molecules. Glycosylation plays a crucial role in many biological processes and can also be used for a variety of industrial applications. Indeed, the attachment of a carbohydrate moiety can significantly improve both the physicochemical and biological properties of small molecules like drugs, cosmetics, fragrances and food ingredients. Unfortunately, only a few biocatalysts are available today that can be used cost-efficiently on an industrial scale. The goal of this research is to explore the use of glycoside phosphorylases for the production of glycosylated compounds. Particular attention will be paid to glycolipid products, which can be used as biosurfactants in a wide range of sectors such as the chemical, cosmetic and detergent industries. Their specific action, low toxicity and biodegradability are the reasons for their popularity as high value microbial products.
Keywords
lactolipid, cellodextrin phosphorylase, cellobiose phosphorylase, loop engineering, glycolipid

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 5.04 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Tran, Hai Giang. 2012. “Improving the Glycosylation Activity of Cellobiose/cellodextrin Phosphorylase Through Enzyme Engineering”. Ghent, Belgium: Ghent University. Faculty of Bioscience Engineering.
APA
Tran, H. G. (2012). Improving the glycosylation activity of cellobiose/cellodextrin phosphorylase through enzyme engineering. Ghent University. Faculty of Bioscience Engineering, Ghent, Belgium.
Vancouver
1.
Tran HG. Improving the glycosylation activity of cellobiose/cellodextrin phosphorylase through enzyme engineering. [Ghent, Belgium]: Ghent University. Faculty of Bioscience Engineering; 2012.
MLA
Tran, Hai Giang. “Improving the Glycosylation Activity of Cellobiose/cellodextrin Phosphorylase Through Enzyme Engineering.” 2012 : n. pag. Print.
@phdthesis{3010085,
  abstract     = {This PhD project is part of a larger project that aims to develop a generic technology for the biocatalytic glycosylation of small molecules. Glycosylation plays a crucial role in many biological processes and can also be used for a variety of industrial applications. Indeed, the attachment of a  carbohydrate moiety can significantly improve both the
physicochemical and biological properties of small molecules like drugs, cosmetics, fragrances and food ingredients. Unfortunately, only a few biocatalysts are available today that can be used cost-efficiently on an industrial scale. The goal of this research is to explore the use of glycoside phosphorylases for the production of glycosylated compounds. Particular attention will be paid to glycolipid products, which can be used as biosurfactants in a wide range of sectors such as the chemical, cosmetic and detergent industries. Their specific action, low toxicity and biodegradability are the reasons for their popularity as high value microbial products.},
  author       = {Tran, Hai Giang},
  isbn         = {9789059895546},
  language     = {eng},
  pages        = {196},
  publisher    = {Ghent University. Faculty of Bioscience Engineering},
  school       = {Ghent University},
  title        = {Improving the glycosylation activity of cellobiose/cellodextrin phosphorylase through enzyme engineering},
  year         = {2012},
}