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Vp1 expression profiles during kernel development in six genotypes of wheat, triticale and rye

Sarah De Laethauwer UGent, Dirk Reheul UGent, Jan De Riek and Geert Haesaert UGent (2012) EUPHYTICA. 188(1). p.61-70
abstract
During the last few decades, the physiological and genetic background of dormancy, and correlated pre-harvest sprouting (PHS) have been intensively investigated. Special attention has often been paid to genetic factors thatmay explain and predict PHS susceptible behaviour. A major candidate is the Vp1 genewhich is involved inembryo development and maturation as well as in dormancy establishment. In this study, Vp1 gene expression during kernel development was studied in wheat, triticale and rye as a potential biomarker for selecting PHS tolerant varieties in cereal breeding programs. Plants of known PHS tolerant and PHS susceptible varieties were grown under controlled conditions from flowering until harvest ripeness. During that period, kernels were regularly harvested for RNA extraction and cDNA synthesis. Calibrated and normalized relative Vp1 expression levels were obtained in an RT-qPCR assay. During kernel development, Vp1 expression levels generally showed a typical peak during the soft dough stage, after which they decreased and remained low until harvestmaturity. Differences in Vp1 expression levels could be observed between the PHS susceptible and PHS tolerant varieties of wheat, with the PHS tolerant variety showing higher levels of relative Vp1 expression compared to the PHS susceptible variety. In triticale, however, this difference was only seen once and could not be confirmed in further experiments. It seems that the Vp1 gene in triticale behaves in a similar way as in rye, in which no specific trends could be observed.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Wheat, Triticale, Rye, Vp1, Kernel development, Pre-harvest sprouting, PREHARVEST SPROUTING TOLERANCE, ABSCISIC-ACID, VIVIPAROUS-1, GENE, DORMANCY, EMBRYOS, MAIZE, CULTIVARS, TRANSCRIPTS, SENSITIVITY
journal title
EUPHYTICA
Euphytica
volume
188
issue
1
pages
61 - 70
Web of Science type
Article
Web of Science id
000314268500007
JCR category
HORTICULTURE
JCR impact factor
1.643 (2012)
JCR rank
7/32 (2012)
JCR quartile
1 (2012)
ISSN
0014-2336
DOI
10.1007/s10681-011-0613-9
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
3009178
handle
http://hdl.handle.net/1854/LU-3009178
date created
2012-10-09 09:00:37
date last changed
2013-07-12 10:50:08
@article{3009178,
  abstract     = {During the last few decades, the physiological and genetic background of dormancy, and correlated pre-harvest sprouting (PHS) have been intensively investigated. Special attention has often been paid to genetic factors thatmay explain and predict PHS susceptible behaviour. A major candidate is the Vp1 genewhich is involved inembryo development and maturation as well as in dormancy establishment. In this study, Vp1 gene expression during kernel development was studied in wheat, triticale and rye as a potential biomarker for selecting PHS tolerant varieties in cereal breeding programs. Plants of known PHS tolerant and PHS susceptible varieties were grown under controlled conditions from flowering until harvest ripeness. During that period, kernels were regularly harvested for RNA extraction and cDNA synthesis. Calibrated and normalized relative Vp1 expression levels were obtained in an RT-qPCR assay. During kernel development, Vp1 expression levels generally showed a typical peak during the soft dough stage, after which they decreased and remained low until harvestmaturity. Differences in Vp1 expression levels could be observed between the PHS susceptible and PHS tolerant varieties of wheat, with the PHS tolerant variety showing higher levels of relative Vp1 expression compared to the PHS susceptible variety. In triticale, however, this difference was only seen once and could not be confirmed in further experiments. It seems that the Vp1 gene in triticale behaves in a similar way as in rye, in which no specific trends could be observed.},
  author       = {De Laethauwer, Sarah and Reheul, Dirk and De Riek, Jan and Haesaert, Geert},
  issn         = {0014-2336},
  journal      = {EUPHYTICA},
  keyword      = {Wheat,Triticale,Rye,Vp1,Kernel development,Pre-harvest sprouting,PREHARVEST SPROUTING TOLERANCE,ABSCISIC-ACID,VIVIPAROUS-1,GENE,DORMANCY,EMBRYOS,MAIZE,CULTIVARS,TRANSCRIPTS,SENSITIVITY},
  language     = {eng},
  number       = {1},
  pages        = {61--70},
  title        = {Vp1 expression profiles during kernel development in six genotypes of wheat, triticale and rye},
  url          = {http://dx.doi.org/10.1007/s10681-011-0613-9},
  volume       = {188},
  year         = {2012},
}

Chicago
De Laethauwer, Sarah, Dirk Reheul, Jan De Riek, and Geert Haesaert. 2012. “Vp1 Expression Profiles During Kernel Development in Six Genotypes of Wheat, Triticale and Rye.” Euphytica 188 (1): 61–70.
APA
De Laethauwer, S., Reheul, D., De Riek, J., & Haesaert, G. (2012). Vp1 expression profiles during kernel development in six genotypes of wheat, triticale and rye. EUPHYTICA, 188(1), 61–70.
Vancouver
1.
De Laethauwer S, Reheul D, De Riek J, Haesaert G. Vp1 expression profiles during kernel development in six genotypes of wheat, triticale and rye. EUPHYTICA. 2012;188(1):61–70.
MLA
De Laethauwer, Sarah, Dirk Reheul, Jan De Riek, et al. “Vp1 Expression Profiles During Kernel Development in Six Genotypes of Wheat, Triticale and Rye.” EUPHYTICA 188.1 (2012): 61–70. Print.