Evaluation of alkyl polyglucoside as an alternative surfactant in the preparation of peptide-loaded nanoparticles
- Author
- Ashok Patel (UGent) , Swati Kulkarni, Tarala D Nandekar and Pradeep R Vavia
- Organization
- Abstract
- The present study was carried out to evaluate potentials of alkyl polyglucoside (APG) as an alternative surfactant/stabilizer in the preparation of peptide-loaded nanoparticles. hGF2 (human gel filtration fraction 2) was used as a model peptide and APG based on C10 fatty alcohol (decyl polylglucoside) was used as a representative surfactant. PLA (poly D,L-lactide) and PLGA (poly D,L-lactide-co-glycolide) based nanoparticles were prepared and the effect of APG on particle size, entrapment efficiency and biological activity was evaluated. At a concentration as low as 0.05% w/v, APG provided an excellent stabilization effect, resulting in nanoparticles with better encapsulation efficiency and particle size well below 450 nm. The effective concentration of APG was found to be much less (10-fold) as compared to PVA. Moreover, APG also showed low residual content as compared to PVA. In-vitro and in-vivo biological activity evaluation confirmed that encapsulated peptide was compatible with APG. These preliminary results suggest that APGs can be used as alternate surfactants for nanoparticle preparation.
- Keywords
- hGF2, Alkyl polyglucosides, residual surfactant content, ELISA, flow cytometry, SOLVENT EVAPORATION METHOD, POLYVINYL-ALCOHOL, CALLITHRIX-JACCHUS, DELIVERY, MICROENCAPSULATION, NIOSOMES, SIZE
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-3007971
- MLA
- Patel, Ashok, et al. “Evaluation of Alkyl Polyglucoside as an Alternative Surfactant in the Preparation of Peptide-Loaded Nanoparticles.” JOURNAL OF MICROENCAPSULATION, vol. 25, no. 8, 2008, pp. 531–40, doi:10.1080/02652040802075526.
- APA
- Patel, A., Kulkarni, S., Nandekar, T. D., & Vavia, P. R. (2008). Evaluation of alkyl polyglucoside as an alternative surfactant in the preparation of peptide-loaded nanoparticles. JOURNAL OF MICROENCAPSULATION, 25(8), 531–540. https://doi.org/10.1080/02652040802075526
- Chicago author-date
- Patel, Ashok, Swati Kulkarni, Tarala D Nandekar, and Pradeep R Vavia. 2008. “Evaluation of Alkyl Polyglucoside as an Alternative Surfactant in the Preparation of Peptide-Loaded Nanoparticles.” JOURNAL OF MICROENCAPSULATION 25 (8): 531–40. https://doi.org/10.1080/02652040802075526.
- Chicago author-date (all authors)
- Patel, Ashok, Swati Kulkarni, Tarala D Nandekar, and Pradeep R Vavia. 2008. “Evaluation of Alkyl Polyglucoside as an Alternative Surfactant in the Preparation of Peptide-Loaded Nanoparticles.” JOURNAL OF MICROENCAPSULATION 25 (8): 531–540. doi:10.1080/02652040802075526.
- Vancouver
- 1.Patel A, Kulkarni S, Nandekar TD, Vavia PR. Evaluation of alkyl polyglucoside as an alternative surfactant in the preparation of peptide-loaded nanoparticles. JOURNAL OF MICROENCAPSULATION. 2008;25(8):531–40.
- IEEE
- [1]A. Patel, S. Kulkarni, T. D. Nandekar, and P. R. Vavia, “Evaluation of alkyl polyglucoside as an alternative surfactant in the preparation of peptide-loaded nanoparticles,” JOURNAL OF MICROENCAPSULATION, vol. 25, no. 8, pp. 531–540, 2008.
@article{3007971,
abstract = {{The present study was carried out to evaluate potentials of alkyl polyglucoside (APG) as an alternative surfactant/stabilizer in the preparation of peptide-loaded nanoparticles. hGF2 (human gel filtration fraction 2) was used as a model peptide and APG based on C10 fatty alcohol (decyl polylglucoside) was used as a representative surfactant. PLA (poly D,L-lactide) and PLGA (poly D,L-lactide-co-glycolide) based nanoparticles were prepared and the effect of APG on particle size, entrapment efficiency and biological activity was evaluated. At a concentration as low as 0.05% w/v, APG provided an excellent stabilization effect, resulting in nanoparticles with better encapsulation efficiency and particle size well below 450 nm. The effective concentration of APG was found to be much less (10-fold) as compared to PVA. Moreover, APG also showed low residual content as compared to PVA. In-vitro and in-vivo biological activity evaluation confirmed that encapsulated peptide was compatible with APG. These preliminary results suggest that APGs can be used as alternate surfactants for nanoparticle preparation.}},
author = {{Patel, Ashok and Kulkarni, Swati and Nandekar, Tarala D and Vavia, Pradeep R}},
issn = {{0265-2048}},
journal = {{JOURNAL OF MICROENCAPSULATION}},
keywords = {{hGF2,Alkyl polyglucosides,residual surfactant content,ELISA,flow cytometry,SOLVENT EVAPORATION METHOD,POLYVINYL-ALCOHOL,CALLITHRIX-JACCHUS,DELIVERY,MICROENCAPSULATION,NIOSOMES,SIZE}},
language = {{eng}},
number = {{8}},
pages = {{531--540}},
title = {{Evaluation of alkyl polyglucoside as an alternative surfactant in the preparation of peptide-loaded nanoparticles}},
url = {{http://doi.org/10.1080/02652040802075526}},
volume = {{25}},
year = {{2008}},
}
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