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Influence of the carbon/nitrogen/phosphorus ratio on polycyclic aromatic hydrocarbon degradation by Mycobacterium and Sphingomonas in soil

Natalie M Leys, Leen Bastiaens, Willy Verstraete UGent and Dirk Springael (2005) APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 66(6). p.726-736
abstract
Biodegradation of polycyclic aromatic hydrocarbons (PAHs) in the environment is often limited due to unfavorable nutrient conditions for the bacteria that use these PAHs as sole source of carbon and energy. Mycobacterium and Sphingomonas are 2 PAH-degrading specialists commonly present in PAH-polluted soil, but not much is known about their specific nutrient requirements. By adding different inorganic supplements of nitrogen (N) and phosphorus (P), affecting the overall carbon/nitrogen/phosphorus ratio of soil in soil slurry degradation tests, we investigated the impact of soil inorganic N and P nutrient conditions on PAH degradation by PAH-degrading Sphingomonas and Mycobacterium strains. The general theoretically calculated C/N/P ratio of 100/10/1 (expressed in moles) allowed rapid PAH metabolization by Sphingomonas and Mycobacterium strains without limitation. In addition, PAH-degradation rate and extent was not affected when ca. ten times lower concentrations of N and P were provided, indicating that Sphingomonas and Mycobacterium strains are capable of metabolizing PAHs under low nutrient conditions. Nor does PAH-degradation seem to be affected by excesses of N and P creating an imbalanced C/N/P ratio. However, supplements of N and P salts increased the salinity of soil slurry solutions and seriously limited or even completely blocked biodegradation.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
CONTAMINATED SOIL, MICROBIAL-DEGRADATION, GROWTH SUBSTRATE, SP LB501T, BIOREMEDIATION, BIODEGRADATION, PHENANTHRENE, STRAIN, MINERALIZATION, SUPPLEMENTS
journal title
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Appl. Microbiol. Biotechnol.
volume
66
issue
6
pages
726-736 pages
Web of Science type
Article
Web of Science id
000227276900018
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
2.586 (2005)
JCR rank
39/135 (2005)
JCR quartile
2 (2005)
ISSN
0175-7598
DOI
10.1007/s00253-004-1766-4
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
300436
handle
http://hdl.handle.net/1854/LU-300436
date created
2005-03-22 14:44:00
date last changed
2016-12-19 15:45:17
@article{300436,
  abstract     = {Biodegradation of polycyclic aromatic hydrocarbons (PAHs) in the environment is often limited due to unfavorable nutrient conditions for the bacteria that use these PAHs as sole source of carbon and energy. Mycobacterium and Sphingomonas are 2 PAH-degrading specialists commonly present in PAH-polluted soil, but not much is known about their specific nutrient requirements. By adding different inorganic supplements of nitrogen (N) and phosphorus (P), affecting the overall carbon/nitrogen/phosphorus ratio of soil in soil slurry degradation tests, we investigated the impact of soil inorganic N and P nutrient conditions on PAH degradation by PAH-degrading Sphingomonas and Mycobacterium strains. The general theoretically calculated C/N/P ratio of 100/10/1 (expressed in moles) allowed rapid PAH metabolization by Sphingomonas and Mycobacterium strains without limitation. In addition, PAH-degradation rate and extent was not affected when ca. ten times lower concentrations of N and P were provided, indicating that Sphingomonas and Mycobacterium strains are capable of metabolizing PAHs under low nutrient conditions. Nor does PAH-degradation seem to be affected by excesses of N and P creating an imbalanced C/N/P ratio. However, supplements of N and P salts increased the salinity of soil slurry solutions and seriously limited or even completely blocked biodegradation.},
  author       = {Leys, Natalie M and Bastiaens, Leen and Verstraete, Willy and Springael, Dirk},
  issn         = {0175-7598},
  journal      = {APPLIED MICROBIOLOGY AND BIOTECHNOLOGY},
  keyword      = {CONTAMINATED SOIL,MICROBIAL-DEGRADATION,GROWTH SUBSTRATE,SP LB501T,BIOREMEDIATION,BIODEGRADATION,PHENANTHRENE,STRAIN,MINERALIZATION,SUPPLEMENTS},
  language     = {eng},
  number       = {6},
  pages        = {726--736},
  title        = {Influence of the carbon/nitrogen/phosphorus ratio on polycyclic aromatic hydrocarbon degradation by Mycobacterium and Sphingomonas in soil},
  url          = {http://dx.doi.org/10.1007/s00253-004-1766-4},
  volume       = {66},
  year         = {2005},
}

Chicago
Leys, Natalie M, Leen Bastiaens, Willy Verstraete, and Dirk Springael. 2005. “Influence of the Carbon/nitrogen/phosphorus Ratio on Polycyclic Aromatic Hydrocarbon Degradation by Mycobacterium and Sphingomonas in Soil.” Applied Microbiology and Biotechnology 66 (6): 726–736.
APA
Leys, N. M., Bastiaens, L., Verstraete, W., & Springael, D. (2005). Influence of the carbon/nitrogen/phosphorus ratio on polycyclic aromatic hydrocarbon degradation by Mycobacterium and Sphingomonas in soil. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 66(6), 726–736.
Vancouver
1.
Leys NM, Bastiaens L, Verstraete W, Springael D. Influence of the carbon/nitrogen/phosphorus ratio on polycyclic aromatic hydrocarbon degradation by Mycobacterium and Sphingomonas in soil. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2005;66(6):726–36.
MLA
Leys, Natalie M, Leen Bastiaens, Willy Verstraete, et al. “Influence of the Carbon/nitrogen/phosphorus Ratio on Polycyclic Aromatic Hydrocarbon Degradation by Mycobacterium and Sphingomonas in Soil.” APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 66.6 (2005): 726–736. Print.