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Novel polymer-polyphenol beads for encapsulation and microreactor applications

Ashok Patel UGent, Jaap Nisjje and Krassimir P Velikov (2011) SOFT MATTER. 7(9). p.4294-4301
abstract
Novel structures with controllable morphology made from natural materials hold promise for several applications in pharmaceuticals, foods and biotechnology field. In this paper, we utilized the strong interactions between epigallocatechin gallate (EGCG), a polyphenol obtained from green tea, and water soluble cellulose ethers like methyl cellulose (MCE) and hydroxyl propyl methylcellulose (HPMC) to fabricate novel beads with controllable core-shell morphology. The novel polymer-polyphenol beads were prepared by simply adding polymer solution dropwise into an aqueous solution of polyphenol. Effect of process variables like concentration and viscosity grades of polymer, reaction time and concentration of EGCG on bead formation was evaluated. Spherical milky white beads with average diameters ranging from 600-2500 mm were obtained that retained shape after freeze drying. Internal structure of beads was studied using automated light microscopy and cold stage scanning electron microscopy (Cryo-SEM). Percent loading of EGCG in the beads was above 25% w/w in all the cases. The release study in simulated intestinal fluid revealed burst release of EGCG (20% in first 5 minutes) followed by sustained release with more than 90% release at the end of 300 minutes. The antioxidant power of EGCG in the beads was effectively maintained in the alkaline conditions with 5-6 fold activity as compared to free EGCG. Further, the temperature triggered release characteristic of beads was explored by encapsulating eucalyptus oil and studying the release of oil using hot stage microscopy. Enzyme immobilisation application of beads was demonstrated by immobilising a-amylase onto the beads and studying its enzyme activity after several washings.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PRECIPITATION, PROLINE-RICH PROTEIN, ABILITY
journal title
SOFT MATTER
Soft Matter
volume
7
issue
9
pages
4294 - 4301
Web of Science type
Article
Web of Science id
000289634000029
JCR category
PHYSICS, MULTIDISCIPLINARY
JCR impact factor
4.39 (2011)
JCR rank
7/82 (2011)
JCR quartile
1 (2011)
ISSN
1744-683X
DOI
10.1039/c1sm05135k
language
English
UGent publication?
no
classification
A1
id
3004264
handle
http://hdl.handle.net/1854/LU-3004264
date created
2012-10-02 13:56:25
date last changed
2012-10-03 10:10:07
@article{3004264,
  abstract     = {Novel structures with controllable morphology made from natural materials hold promise for several applications in pharmaceuticals, foods and biotechnology field. In this paper, we utilized the strong interactions between epigallocatechin gallate (EGCG), a polyphenol obtained from green tea, and water soluble cellulose ethers like methyl cellulose (MCE) and hydroxyl propyl methylcellulose (HPMC) to fabricate novel beads with controllable core-shell morphology. The novel polymer-polyphenol beads were prepared by simply adding polymer solution dropwise into an aqueous solution of polyphenol. Effect of process variables like concentration and viscosity grades of polymer, reaction time and concentration of EGCG on bead formation was evaluated. Spherical milky white beads with average diameters ranging from 600-2500 mm were obtained that retained shape after freeze drying. Internal structure of beads was studied using automated light microscopy and cold stage scanning electron microscopy (Cryo-SEM). Percent loading of EGCG in the beads was above 25\% w/w in all the cases. The release study in simulated intestinal fluid revealed burst release of EGCG (20\% in first 5 minutes) followed by sustained release with more than 90\% release at the end of 300 minutes. The antioxidant power of EGCG in the beads was effectively maintained in the alkaline conditions with 5-6 fold activity as compared to free EGCG. Further, the temperature triggered release characteristic of beads was explored by encapsulating eucalyptus oil and studying the release of oil using hot stage microscopy. Enzyme immobilisation application of beads was demonstrated by immobilising a-amylase onto the beads and studying its enzyme activity after several washings.},
  author       = {Patel, Ashok and Nisjje, Jaap and Velikov, Krassimir P},
  issn         = {1744-683X},
  journal      = {SOFT MATTER},
  keyword      = {PRECIPITATION,PROLINE-RICH PROTEIN,ABILITY},
  language     = {eng},
  number       = {9},
  pages        = {4294--4301},
  title        = {Novel polymer-polyphenol beads for encapsulation and microreactor applications},
  url          = {http://dx.doi.org/10.1039/c1sm05135k},
  volume       = {7},
  year         = {2011},
}

Chicago
Patel, Ashok, Jaap Nisjje, and Krassimir P Velikov. 2011. “Novel Polymer-polyphenol Beads for Encapsulation and Microreactor Applications.” Soft Matter 7 (9): 4294–4301.
APA
Patel, A., Nisjje, J., & Velikov, K. P. (2011). Novel polymer-polyphenol beads for encapsulation and microreactor applications. SOFT MATTER, 7(9), 4294–4301.
Vancouver
1.
Patel A, Nisjje J, Velikov KP. Novel polymer-polyphenol beads for encapsulation and microreactor applications. SOFT MATTER. 2011;7(9):4294–301.
MLA
Patel, Ashok, Jaap Nisjje, and Krassimir P Velikov. “Novel Polymer-polyphenol Beads for Encapsulation and Microreactor Applications.” SOFT MATTER 7.9 (2011): 4294–4301. Print.