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Differential proteomic analysis of the response of Stenotrophomonas maltophilia to imipenem

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Abstract
This study represents two different large-scale proteomic experiments analyzing the antibiotic response and the mechanisms of production of beta-lactamases in the nosocomial pathogen Stenotrophomonas maltophilia. Two-dimensional gel electrophoresis on the cytoplasmic protein fraction, together with iTRAQA (R) differential labeling and 2-D liquid chromatographic separation (2D-LC) MS/MS on the enriched membrane protein fraction, revealed 73 proteins with a change in abundance upon imipenem challenge. These proteins belong to several different functional pathways. We observe an increase in beta-lactamase production as well as in proteins important for their function in the periplasm. The up-regulation of the L1 and L2 beta-lactamases, along with their activator LysR transcriptional factor AmpR, is linked to an increase in proteins responsible for peptidoglycan remodeling and stress response. The interesting identification of an increase in abundance after treatment of the two-component GGDEF signaling protein and an integral membrane sensor signal transduction histidine kinase, indicates that induction of the beta-lactamases is not restricted to the ampR-ampD-ampG pathway. This is the first proteomic study in S. maltophilia upon imipenem stimulation to further unravel the cellular adaptation resulting in beta-lactamase production.
Keywords
Opportunistic pathogen, 2-DE, Resistance, Antibiotics, LCMS, BETA-LACTAMASE PRODUCTION, OUTER-MEMBRANE PROTEINS, GRAM-NEGATIVE BACTERIA, ESCHERICHIA-COLI, SACCHAROMYCES-CEREVISIAE, ANTIBIOTIC RESEARCH, DRUG-RESISTANCE, EXPRESSION, L1, INDUCTION

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MLA
Van Oudenhove, Laurence, Kris De Vriendt, Jozef Van Beeumen, et al. “Differential Proteomic Analysis of the Response of Stenotrophomonas Maltophilia to Imipenem.” APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 95.3 (2012): 717–733. Print.
APA
Van Oudenhove, L., De Vriendt, K., Van Beeumen, J., Mercuri, P. S., & Devreese, B. (2012). Differential proteomic analysis of the response of Stenotrophomonas maltophilia to imipenem. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 95(3), 717–733.
Chicago author-date
Van Oudenhove, Laurence, Kris De Vriendt, Jozef Van Beeumen, Paola Sandra Mercuri, and Bart Devreese. 2012. “Differential Proteomic Analysis of the Response of Stenotrophomonas Maltophilia to Imipenem.” Applied Microbiology and Biotechnology 95 (3): 717–733.
Chicago author-date (all authors)
Van Oudenhove, Laurence, Kris De Vriendt, Jozef Van Beeumen, Paola Sandra Mercuri, and Bart Devreese. 2012. “Differential Proteomic Analysis of the Response of Stenotrophomonas Maltophilia to Imipenem.” Applied Microbiology and Biotechnology 95 (3): 717–733.
Vancouver
1.
Van Oudenhove L, De Vriendt K, Van Beeumen J, Mercuri PS, Devreese B. Differential proteomic analysis of the response of Stenotrophomonas maltophilia to imipenem. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2012;95(3):717–33.
IEEE
[1]
L. Van Oudenhove, K. De Vriendt, J. Van Beeumen, P. S. Mercuri, and B. Devreese, “Differential proteomic analysis of the response of Stenotrophomonas maltophilia to imipenem,” APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 95, no. 3, pp. 717–733, 2012.
@article{3002872,
  abstract     = {This study represents two different large-scale proteomic experiments analyzing the antibiotic response and the mechanisms of production of beta-lactamases in the nosocomial pathogen Stenotrophomonas maltophilia. Two-dimensional gel electrophoresis on the cytoplasmic protein fraction, together with iTRAQA (R) differential labeling and 2-D liquid chromatographic separation (2D-LC) MS/MS on the enriched membrane protein fraction, revealed 73 proteins with a change in abundance upon imipenem challenge. These proteins belong to several different functional pathways. We observe an increase in beta-lactamase production as well as in proteins important for their function in the periplasm. The up-regulation of the L1 and L2 beta-lactamases, along with their activator LysR transcriptional factor AmpR, is linked to an increase in proteins responsible for peptidoglycan remodeling and stress response. The interesting identification of an increase in abundance after treatment of the two-component GGDEF signaling protein and an integral membrane sensor signal transduction histidine kinase, indicates that induction of the beta-lactamases is not restricted to the ampR-ampD-ampG pathway. This is the first proteomic study in S. maltophilia upon imipenem stimulation to further unravel the cellular adaptation resulting in beta-lactamase production.},
  author       = {Van Oudenhove, Laurence and De Vriendt, Kris and Van Beeumen, Jozef and Mercuri, Paola Sandra and Devreese, Bart},
  issn         = {0175-7598},
  journal      = {APPLIED MICROBIOLOGY AND BIOTECHNOLOGY},
  keywords     = {Opportunistic pathogen,2-DE,Resistance,Antibiotics,LCMS,BETA-LACTAMASE PRODUCTION,OUTER-MEMBRANE PROTEINS,GRAM-NEGATIVE BACTERIA,ESCHERICHIA-COLI,SACCHAROMYCES-CEREVISIAE,ANTIBIOTIC RESEARCH,DRUG-RESISTANCE,EXPRESSION,L1,INDUCTION},
  language     = {eng},
  number       = {3},
  pages        = {717--733},
  title        = {Differential proteomic analysis of the response of Stenotrophomonas maltophilia to imipenem},
  url          = {http://dx.doi.org/10.1007/s00253-012-4167-0},
  volume       = {95},
  year         = {2012},
}

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