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Quantification of IFNγ- and IL17-producing cells after stimulation with citrullinated proteins in healthy subjects and RA patients

Katleen Van Steendam UGent, Marlies De Ceuleneer UGent, Kelly Tilleman UGent, Dirk Elewaut UGent, Filip De Keyser UGent and Dieter Deforce UGent (2013) RHEUMATOLOGY INTERNATIONAL. 33(10). p.2661-2664
abstract
Antibodies against citrullinated proteins are highly specific for rheumatoid arthritis (RA) and are currently used as a diagnostic marker. In this study, we wanted to quantify the numbers of T cells that react to a wide range of citrullinated proteins in a wide range of HLA-DR subtypes in order to investigate whether citrullination might create T-cell neo-epitopes and could initiate a universal T-cell response. Therefore, PBMCs from healthy volunteers and RA patients were stimulated with a citrullinated and non-citrullinated cell extract on IFN gamma-ELISpot. We found a significantly higher number of IFN gamma-secreting cells after stimulation with citrullinated proteins compared to non-citrullinated proteins in RA patients (1:14,441 cells vs. 1:32,880 cells) as well as in healthy subjects (1:6,261 reactive cells compared to 1:16,212 cells). Additionally, a higher number of IL17-secreting cells were found after stimulation with citrullinated proteins compared to their non-citrullinated counterparts. Our data indicate that citrulline-dependent T-cell response is not restricted to RA patients but that citrullination as such gives rise to a universal break in tolerance.
Please use this url to cite or link to this publication:
author
organization
alternative title
Quantification of IFN-gamma- and IL17-producing cells after stimulation with citrullinated proteins in healthy subjects and RA patients
year
type
journalArticle (original)
publication status
published
subject
keyword
Rheumatoid arthritis, Citrullination, ELISpot, T cells, RHEUMATOID-ARTHRITIS, T-CELLS
journal title
RHEUMATOLOGY INTERNATIONAL
Rheumatol. Int.
volume
33
issue
10
pages
2661 - 2664
Web of Science type
Article
Web of Science id
000324824500029
JCR category
RHEUMATOLOGY
JCR impact factor
1.627 (2013)
JCR rank
22/30 (2013)
JCR quartile
3 (2013)
ISSN
0172-8172
DOI
10.1007/s00296-012-2470-9
language
English
UGent publication?
yes
classification
A1
additional info
the first two authors contributed equally to this work
copyright statement
I have retained and own the full copyright for this publication
id
2998858
handle
http://hdl.handle.net/1854/LU-2998858
date created
2012-09-25 10:54:55
date last changed
2015-02-06 10:28:06
@article{2998858,
  abstract     = {Antibodies against citrullinated proteins are highly specific for rheumatoid arthritis (RA) and are currently used as a diagnostic marker. In this study, we wanted to quantify the numbers of T cells that react to a wide range of citrullinated proteins in a wide range of HLA-DR subtypes in order to investigate whether citrullination might create T-cell neo-epitopes and could initiate a universal T-cell response. Therefore, PBMCs from healthy volunteers and RA patients were stimulated with a citrullinated and non-citrullinated cell extract on IFN gamma-ELISpot. We found a significantly higher number of IFN gamma-secreting cells after stimulation with citrullinated proteins compared to non-citrullinated proteins in RA patients (1:14,441 cells vs. 1:32,880 cells) as well as in healthy subjects (1:6,261 reactive cells compared to 1:16,212 cells). Additionally, a higher number of IL17-secreting cells were found after stimulation with citrullinated proteins compared to their non-citrullinated counterparts. Our data indicate that citrulline-dependent T-cell response is not restricted to RA patients but that citrullination as such gives rise to a universal break in tolerance.},
  author       = {Van Steendam, Katleen and De Ceuleneer, Marlies and Tilleman, Kelly and Elewaut, Dirk and De Keyser, Filip and Deforce, Dieter},
  issn         = {0172-8172},
  journal      = {RHEUMATOLOGY INTERNATIONAL},
  keyword      = {Rheumatoid arthritis,Citrullination,ELISpot,T cells,RHEUMATOID-ARTHRITIS,T-CELLS},
  language     = {eng},
  number       = {10},
  pages        = {2661--2664},
  title        = {Quantification of IFN\ensuremath{\gamma}- and IL17-producing cells after stimulation with citrullinated proteins in healthy subjects and RA patients},
  url          = {http://dx.doi.org/10.1007/s00296-012-2470-9},
  volume       = {33},
  year         = {2013},
}

Chicago
Van Steendam, Katleen, Marlies De Ceuleneer, Kelly Tilleman, Dirk Elewaut, Filip De Keyser, and Dieter Deforce. 2013. “Quantification of IFNγ- and IL17-producing Cells After Stimulation with Citrullinated Proteins in Healthy Subjects and RA Patients.” Rheumatology International 33 (10): 2661–2664.
APA
Van Steendam, K., De Ceuleneer, M., Tilleman, K., Elewaut, D., De Keyser, F., & Deforce, D. (2013). Quantification of IFNγ- and IL17-producing cells after stimulation with citrullinated proteins in healthy subjects and RA patients. RHEUMATOLOGY INTERNATIONAL, 33(10), 2661–2664.
Vancouver
1.
Van Steendam K, De Ceuleneer M, Tilleman K, Elewaut D, De Keyser F, Deforce D. Quantification of IFNγ- and IL17-producing cells after stimulation with citrullinated proteins in healthy subjects and RA patients. RHEUMATOLOGY INTERNATIONAL. 2013;33(10):2661–4.
MLA
Van Steendam, Katleen, Marlies De Ceuleneer, Kelly Tilleman, et al. “Quantification of IFNγ- and IL17-producing Cells After Stimulation with Citrullinated Proteins in Healthy Subjects and RA Patients.” RHEUMATOLOGY INTERNATIONAL 33.10 (2013): 2661–2664. Print.