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Belo Horizonte virus: a vaccinia-like virus lacking the A-type inclusion body gene isolated from infected mice

(2004) JOURNAL OF GENERAL VIROLOGY. 85(7). p.2015-2021
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Abstract
Here is described the isolation of a naturally occurring A-type inclusion body (ATI)-negative vaccinia-like virus, Belo Horizonte virus (VBH), obtained from a mousepox-like outbreak in Brazil. The isolated virus was identified and characterized as an orthopoxvirus by conventional methods. Molecular characterization of the virus was done by DNA cross-hybridization using Vaccinia virus (VACV) DNA. In addition, conserved orthopoxvirus genes such as vaccinia growth factor, thymidine kinase and haemagglutinin were amplified by PCR and sequenced. All sequences presented high similarity to VACV genes. Based on the sequences, phenograms were constructed for comparison with other poxviruses; VBH clustered consistently with VACV strains. Attempts to amplify the ATI gene (ati) by PCR, currently used to identify orthopoxviruses, were unsuccessful. Results presented here suggest that most of the ati gene is deleted in the VBH genome.
Keywords
EVOLUTIONARY DISTANCE, POLYMERASE CHAIN-REACTION, MOUSEPOX VIRUS, COWPOX VIRUS, DNA, PROTEIN, SEQUENCE, POXVIRUS, GENOME, ALIGNMENT

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Citation

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Chicago
Trindade, Giliane S, Flávio G da Fonseca, João T Marques, Sueli Diniz, Juliana A Leite, Stefanie De Bodt, Yves Van de Peer, Cláudio A Bonjardim, Paulo CP Ferreira, and Erna G Kroon. 2004. “Belo Horizonte Virus: a Vaccinia-like Virus Lacking the A-type Inclusion Body Gene Isolated from Infected Mice.” Journal of General Virology 85 (7): 2015–2021.
APA
Trindade, G. S., da Fonseca, F. G., Marques, J. T., Diniz, S., Leite, J. A., De Bodt, S., Van de Peer, Y., et al. (2004). Belo Horizonte virus: a vaccinia-like virus lacking the A-type inclusion body gene isolated from infected mice. JOURNAL OF GENERAL VIROLOGY, 85(7), 2015–2021.
Vancouver
1.
Trindade GS, da Fonseca FG, Marques JT, Diniz S, Leite JA, De Bodt S, et al. Belo Horizonte virus: a vaccinia-like virus lacking the A-type inclusion body gene isolated from infected mice. JOURNAL OF GENERAL VIROLOGY. 2004;85(7):2015–21.
MLA
Trindade, Giliane S, Flávio G da Fonseca, João T Marques, et al. “Belo Horizonte Virus: a Vaccinia-like Virus Lacking the A-type Inclusion Body Gene Isolated from Infected Mice.” JOURNAL OF GENERAL VIROLOGY 85.7 (2004): 2015–2021. Print.
@article{299777,
  abstract     = {Here is described the isolation of a naturally occurring A-type inclusion body (ATI)-negative vaccinia-like virus, Belo Horizonte virus (VBH), obtained from a mousepox-like outbreak in Brazil. The isolated virus was identified and characterized as an orthopoxvirus by conventional methods. Molecular characterization of the virus was done by DNA cross-hybridization using Vaccinia virus (VACV) DNA. In addition, conserved orthopoxvirus genes such as vaccinia growth factor, thymidine kinase and haemagglutinin were amplified by PCR and sequenced. All sequences presented high similarity to VACV genes. Based on the sequences, phenograms were constructed for comparison with other poxviruses; VBH clustered consistently with VACV strains. Attempts to amplify the ATI gene (ati) by PCR, currently used to identify orthopoxviruses, were unsuccessful. Results presented here suggest that most of the ati gene is deleted in the VBH genome.},
  author       = {Trindade, Giliane S and da Fonseca, Flávio G and Marques, João T and Diniz, Sueli and Leite, Juliana A and De Bodt, Stefanie and Van de Peer, Yves and Bonjardim, Cláudio A and Ferreira, Paulo CP and Kroon, Erna G},
  issn         = {0022-1317},
  journal      = {JOURNAL OF GENERAL VIROLOGY},
  keywords     = {EVOLUTIONARY DISTANCE,POLYMERASE CHAIN-REACTION,MOUSEPOX VIRUS,COWPOX VIRUS,DNA,PROTEIN,SEQUENCE,POXVIRUS,GENOME,ALIGNMENT},
  language     = {eng},
  number       = {7},
  pages        = {2015--2021},
  title        = {Belo Horizonte virus: a vaccinia-like virus lacking the A-type inclusion body gene isolated from infected mice},
  url          = {http://dx.doi.org/10.1099/vir.0.79840-0},
  volume       = {85},
  year         = {2004},
}

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