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ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis

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Abstract
Auxin is a key coordinative signal required for many aspects of plant development and its levels are controlled by auxin metabolism and intercellular auxin transport. Here we find that a member of PIN auxin transporter family, PIN8 is expressed in male gametophyte of Arabidopsis thaliana and has a crucial role in pollen development and functionality. Ectopic expression in sporophytic tissues establishes a role of PIN8 in regulating auxin homoeostasis and metabolism. PIN8 co-localizes with PIN5 to the endoplasmic reticulum (ER) where it acts as an auxin transporter. Genetic analyses reveal an antagonistic action of PIN5 and PIN8 in the regulation of intracellular auxin homoeostasis and gametophyte as well as sporophyte development. Our results reveal a role of the auxin transport in male gametophyte development in which the distinct actions of ER-localized PIN transporters regulate cellular auxin homoeostasis and maintain the auxin levels optimal for pollen development and pollen tube growth.
Keywords
BIOSYNTHESIS, EXPRESSION, EFFLUX, THALIANA, UDP-GLUCOSE, POLLEN GERMINATION, PLANT DEVELOPMENT, ENDOPLASMIC-RETICULUM, GRADIENTS, MEMBRANE

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MLA
Ding, Zhaojun, Bangjun Wang, Ignacio Moreno, et al. “ER-localized Auxin Transporter PIN8 Regulates Auxin Homeostasis and Male Gametophyte Development in Arabidopsis.” NATURE COMMUNICATIONS 3 (2012): n. pag. Print.
APA
Ding, Z., Wang, B., Moreno, I., Dupláková, N., Simon, S., Carraro, N., Reemmer, J., et al. (2012). ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis. NATURE COMMUNICATIONS, 3.
Chicago author-date
Ding, Zhaojun, Bangjun Wang, Ignacio Moreno, Nikoleta Dupláková, Sibu Simon, Nicola Carraro, Jesica Reemmer, et al. 2012. “ER-localized Auxin Transporter PIN8 Regulates Auxin Homeostasis and Male Gametophyte Development in Arabidopsis.” Nature Communications 3.
Chicago author-date (all authors)
Ding, Zhaojun, Bangjun Wang, Ignacio Moreno, Nikoleta Dupláková, Sibu Simon, Nicola Carraro, Jesica Reemmer, Aleš Penčik, Xu Chen, Ricardo Tejos Ulloa, Petr Skůpa, Stephan Pollmann, Jozef Mravec, Jan Petrášek, Eva Zažimalová, David Honys, Jakub Rolčik, Angus Murphy, Ariel Orellana, Markus Geisler, and Jiri Friml. 2012. “ER-localized Auxin Transporter PIN8 Regulates Auxin Homeostasis and Male Gametophyte Development in Arabidopsis.” Nature Communications 3.
Vancouver
1.
Ding Z, Wang B, Moreno I, Dupláková N, Simon S, Carraro N, et al. ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis. NATURE COMMUNICATIONS. 2012;3.
IEEE
[1]
Z. Ding et al., “ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis,” NATURE COMMUNICATIONS, vol. 3, 2012.
@article{2997319,
  abstract     = {Auxin is a key coordinative signal required for many aspects of plant development and its levels are controlled by auxin metabolism and intercellular auxin transport. Here we find that a member of PIN auxin transporter family, PIN8 is expressed in male gametophyte of Arabidopsis thaliana and has a crucial role in pollen development and functionality. Ectopic expression in sporophytic tissues establishes a role of PIN8 in regulating auxin homoeostasis and metabolism. PIN8 co-localizes with PIN5 to the endoplasmic reticulum (ER) where it acts as an auxin transporter. Genetic analyses reveal an antagonistic action of PIN5 and PIN8 in the regulation of intracellular auxin homoeostasis and gametophyte as well as sporophyte development. Our results reveal a role of the auxin transport in male gametophyte development in which the distinct actions of ER-localized PIN transporters regulate cellular auxin homoeostasis and maintain the auxin levels optimal for pollen development and pollen tube growth.},
  articleno    = {941},
  author       = {Ding, Zhaojun and Wang, Bangjun and Moreno, Ignacio and Dupláková, Nikoleta and Simon, Sibu and Carraro, Nicola and Reemmer, Jesica and Penčik, Aleš and Chen, Xu and Tejos Ulloa, Ricardo and Skůpa, Petr and Pollmann, Stephan and Mravec, Jozef and Petrášek, Jan and Zažimalová, Eva and Honys, David and Rolčik, Jakub and Murphy, Angus and Orellana, Ariel and Geisler, Markus and Friml, Jiri},
  issn         = {2041-1723},
  journal      = {NATURE COMMUNICATIONS},
  keywords     = {BIOSYNTHESIS,EXPRESSION,EFFLUX,THALIANA,UDP-GLUCOSE,POLLEN GERMINATION,PLANT DEVELOPMENT,ENDOPLASMIC-RETICULUM,GRADIENTS,MEMBRANE},
  language     = {eng},
  pages        = {9},
  title        = {ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis},
  url          = {http://dx.doi.org/10.1038/ncomms1941},
  volume       = {3},
  year         = {2012},
}

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