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Disorganization of cortical microtubules stimulates tangential expansion and reduces the uniformity of cellulose microfibril alignment among cells in the root of Arabidopsis

Tobias I Baskin, Gerrit Beemster UGent, Jan E Judy-March and Françoise Marga (2004) PLANT PHYSIOLOGY. 135(4). p.2279-2290
abstract
To test the role of cortical microtubules in aligning cellulose microfibrils and controlling anisotropic expansion, we exposed Arabidopsis thaliana roots to moderate levels of the microtubule inhibitor, oryzalin. After 2 d of treatment, roots grow at approximately steady state. At that time, the spatial profiles of relative expansion rate in length and diameter were quantified, and roots were cryofixed, freeze-substituted, embedded in plastic, and sectioned. The angular distribution of microtubules as a function of distance from the tip was quantified from antitubulin immunofluorescence images. In alternate sections, the overall amount of alignment among microfibrils and their mean orientation as a function of position was quantified with polarized-light microscopy. The spatial profiles of relative expansion show that the drug affects relative elongation and tangential expansion rates independently. The microtubule distributions averaged to transverse in the growth zone for all treatments, but on oryzalin the distributions became broad, indicating poorly organized arrays. At a subcellular scale, cellulose microfibrils in oryzalin-treated roots were as well aligned as in controls; however, the mean alignment direction, while consistently transverse in the controls, was increasingly variable with oryzalin concentration, meaning that microfibril orientation in one location tended to differ from that of a neighboring location. This conclusion was confirmed by direct observations of microfibrils with field-emission scanning electron microscopy. Taken together, these results suggest that cortical microtubules ensure microfibrils are aligned consistently across the organ, thereby endowing the organ with a uniform mechanical structure.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
FINE-STRUCTURE, WALL MICROFIBRILS, COLCHICINE, SPATIAL-DISTRIBUTION, GROWTH ANISOTROPY, STRESSED MAIZE ROOTS, ORIENTATION, DEPOSITION, DISRUPTION, ORYZALIN
journal title
PLANT PHYSIOLOGY
Plant Physiol.
volume
135
issue
4
pages
2279 - 2290
Web of Science type
Article
Web of Science id
000223482400039
JCR category
PLANT SCIENCES
JCR impact factor
5.881 (2004)
JCR rank
7/137 (2004)
JCR quartile
1 (2004)
ISSN
0032-0889
DOI
10.1104/pp.104.040493
language
English
UGent publication?
no
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
299716
handle
http://hdl.handle.net/1854/LU-299716
date created
2005-03-09 18:00:00
date last changed
2016-12-19 15:45:17
@article{299716,
  abstract     = {To test the role of cortical microtubules in aligning cellulose microfibrils and controlling anisotropic expansion, we exposed Arabidopsis thaliana roots to moderate levels of the microtubule inhibitor, oryzalin. After 2 d of treatment, roots grow at approximately steady state. At that time, the spatial profiles of relative expansion rate in length and diameter were quantified, and roots were cryofixed, freeze-substituted, embedded in plastic, and sectioned. The angular distribution of microtubules as a function of distance from the tip was quantified from antitubulin immunofluorescence images. In alternate sections, the overall amount of alignment among microfibrils and their mean orientation as a function of position was quantified with polarized-light microscopy. The spatial profiles of relative expansion show that the drug affects relative elongation and tangential expansion rates independently. The microtubule distributions averaged to transverse in the growth zone for all treatments, but on oryzalin the distributions became broad, indicating poorly organized arrays. At a subcellular scale, cellulose microfibrils in oryzalin-treated roots were as well aligned as in controls; however, the mean alignment direction, while consistently transverse in the controls, was increasingly variable with oryzalin concentration, meaning that microfibril orientation in one location tended to differ from that of a neighboring location. This conclusion was confirmed by direct observations of microfibrils with field-emission scanning electron microscopy. Taken together, these results suggest that cortical microtubules ensure microfibrils are aligned consistently across the organ, thereby endowing the organ with a uniform mechanical structure.},
  author       = {Baskin, Tobias I and Beemster, Gerrit and Judy-March, Jan E and Marga, Fran\c{c}oise},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keyword      = {FINE-STRUCTURE,WALL MICROFIBRILS,COLCHICINE,SPATIAL-DISTRIBUTION,GROWTH ANISOTROPY,STRESSED MAIZE ROOTS,ORIENTATION,DEPOSITION,DISRUPTION,ORYZALIN},
  language     = {eng},
  number       = {4},
  pages        = {2279--2290},
  title        = {Disorganization of cortical microtubules stimulates tangential expansion and reduces the uniformity of cellulose microfibril alignment among cells in the root of Arabidopsis},
  url          = {http://dx.doi.org/10.1104/pp.104.040493},
  volume       = {135},
  year         = {2004},
}

Chicago
Baskin, Tobias I, Gerrit Beemster, Jan E Judy-March, and Françoise Marga. 2004. “Disorganization of Cortical Microtubules Stimulates Tangential Expansion and Reduces the Uniformity of Cellulose Microfibril Alignment Among Cells in the Root of Arabidopsis.” Plant Physiology 135 (4): 2279–2290.
APA
Baskin, T. I., Beemster, G., Judy-March, J. E., & Marga, F. (2004). Disorganization of cortical microtubules stimulates tangential expansion and reduces the uniformity of cellulose microfibril alignment among cells in the root of Arabidopsis. PLANT PHYSIOLOGY, 135(4), 2279–2290.
Vancouver
1.
Baskin TI, Beemster G, Judy-March JE, Marga F. Disorganization of cortical microtubules stimulates tangential expansion and reduces the uniformity of cellulose microfibril alignment among cells in the root of Arabidopsis. PLANT PHYSIOLOGY. 2004;135(4):2279–90.
MLA
Baskin, Tobias I, Gerrit Beemster, Jan E Judy-March, et al. “Disorganization of Cortical Microtubules Stimulates Tangential Expansion and Reduces the Uniformity of Cellulose Microfibril Alignment Among Cells in the Root of Arabidopsis.” PLANT PHYSIOLOGY 135.4 (2004): 2279–2290. Print.