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Influence of reducing agents on the integrity of selenocompounds: exploratory work for selenoproteome analysis

Cyrille C Chéry, Emmie Dumont UGent, Luc Moens UGent, Frank Vanhaecke UGent and Rita Cornelis UGent (2005) JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY. 20(2). p.118-120
abstract
Three selenium species ( selenomethionine, selenocystine and selenocystamine) were studied for their behaviour in the presence of the reducing agents dithiothreitol and tributylphosphine, and of a derivatising agent, iodoacetic acid. These chemicals are commonly used in proteome analysis by means of gel electrophoresis. To study the effect of these chemicals on the selenospecies, two separation techniques were used, capillary electrophoresis and liquid chromatography, both with ICP-MS as an element-specific detector. Electrospray-mass spectrometry provided molecular information. Tributylphosphine (TBT) was shown to partly reduce selenocystine to selenocysteine, and iodoacetic acid to derivatise all the species, with conservation of the species information. Dithiothreitol (DTT), on the contrary, partly sequestered selenium from the species to form a DTT - Se molecule.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
GEL-ELECTROPHORESIS, PLASMA-MASS SPECTROMETRY, SELENIZED YEAST, SPECIATION, MS
journal title
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
J. Anal. At. Spectrom.
volume
20
issue
2
pages
118 - 120
Web of Science type
Article
Web of Science id
000226523400007
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
3.64 (2005)
JCR rank
4/69 (2005)
JCR quartile
1 (2005)
ISSN
0267-9477
DOI
10.1039/b412754d
language
English
UGent publication?
yes
classification
A1
id
299314
handle
http://hdl.handle.net/1854/LU-299314
date created
2005-03-01 09:14:00
date last changed
2012-11-12 12:53:13
@article{299314,
  abstract     = {Three selenium species ( selenomethionine, selenocystine and selenocystamine) were studied for their behaviour in the presence of the reducing agents dithiothreitol and tributylphosphine, and of a derivatising agent, iodoacetic acid. These chemicals are commonly used in proteome analysis by means of gel electrophoresis. To study the effect of these chemicals on the selenospecies, two separation techniques were used, capillary electrophoresis and liquid chromatography, both with ICP-MS as an element-specific detector. Electrospray-mass spectrometry provided molecular information. Tributylphosphine (TBT) was shown to partly reduce selenocystine to selenocysteine, and iodoacetic acid to derivatise all the species, with conservation of the species information. Dithiothreitol (DTT), on the contrary, partly sequestered selenium from the species to form a DTT - Se molecule.},
  author       = {Ch{\'e}ry, Cyrille C and Dumont, Emmie and Moens, Luc and Vanhaecke, Frank and Cornelis, Rita},
  issn         = {0267-9477},
  journal      = {JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY},
  keyword      = {GEL-ELECTROPHORESIS,PLASMA-MASS SPECTROMETRY,SELENIZED YEAST,SPECIATION,MS},
  language     = {eng},
  number       = {2},
  pages        = {118--120},
  title        = {Influence of reducing agents on the integrity of selenocompounds: exploratory work for selenoproteome analysis},
  url          = {http://dx.doi.org/10.1039/b412754d},
  volume       = {20},
  year         = {2005},
}

Chicago
Chéry, Cyrille C, Emmie Dumont, Luc Moens, Frank Vanhaecke, and Rita Cornelis. 2005. “Influence of Reducing Agents on the Integrity of Selenocompounds: Exploratory Work for Selenoproteome Analysis.” Journal of Analytical Atomic Spectrometry 20 (2): 118–120.
APA
Chéry, C. C., Dumont, E., Moens, L., Vanhaecke, F., & Cornelis, R. (2005). Influence of reducing agents on the integrity of selenocompounds: exploratory work for selenoproteome analysis. JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY, 20(2), 118–120.
Vancouver
1.
Chéry CC, Dumont E, Moens L, Vanhaecke F, Cornelis R. Influence of reducing agents on the integrity of selenocompounds: exploratory work for selenoproteome analysis. JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY. 2005;20(2):118–20.
MLA
Chéry, Cyrille C, Emmie Dumont, Luc Moens, et al. “Influence of Reducing Agents on the Integrity of Selenocompounds: Exploratory Work for Selenoproteome Analysis.” JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY 20.2 (2005): 118–120. Print.